Fine-mapping the wheat Snn1 locus conferring sensitivity to the Parastagonospora nodorum necrotrophic effector SnTox1 using an eight founder multiparent advanced generation inter-cross population
The necrotrophic fungus Parastagonospora nodorum is an important pathogen of one of the world’s most economically important cereal crops, wheat (Triticum aestivum L.). P. nodorum produces necrotrophic protein effectors that mediate host cell death, providing nutrients for continuation of the infecti...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Published: |
2015
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| Online Access: | http://hdl.handle.net/20.500.11937/46450 |
| _version_ | 1848757560475123712 |
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| author | Cockram, J. Scuderi, A. Barber, T. Furuki, E. Gardner, K. Gosman, N. Kowalczyk, R. Phan, H. Rose, G. Tan, K. Oliver, Richard Mackay, I. |
| author_facet | Cockram, J. Scuderi, A. Barber, T. Furuki, E. Gardner, K. Gosman, N. Kowalczyk, R. Phan, H. Rose, G. Tan, K. Oliver, Richard Mackay, I. |
| author_sort | Cockram, J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The necrotrophic fungus Parastagonospora nodorum is an important pathogen of one of the world’s most economically important cereal crops, wheat (Triticum aestivum L.). P. nodorum produces necrotrophic protein effectors that mediate host cell death, providing nutrients for continuation of the infection process. The recent discovery of pathogen effectors has revolutionized disease resistance breeding for necrotrophic diseases in crop species, allowing often complex genetic resistance mechanisms to be broken down into constituent parts. To date, three effectors have been identified in P. nodorum. Here we use the effector, SnTox1, to screen 642 progeny from an eight-parent multiparent advanced generation inter-cross (i.e., MAGIC) population, genotyped with a 90,000-feature single-nucleotide polymorphism array. The MAGIC founders showed a range of sensitivity to SnTox1, with transgressive segregation evident in the progeny. SnTox1 sensitivity showed high heritability, with quantitative trait locus analyses fine-mapping the Snn1 locus to the short arm of chromosome 1B. In addition, a previously undescribed SnTox1 sensitivity locus was identified on the long arm of chromosome 5A, termed here QSnn.niab-5A.1. The peak single-nucleotide polymorphism for the Snn1 locus was converted to the KASP genotyping platform, providing breeders and researchers a simple and cheap diagnostic marker for allelic state at Snn1. |
| first_indexed | 2025-11-14T09:30:02Z |
| format | Journal Article |
| id | curtin-20.500.11937-46450 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:30:02Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-464502017-09-13T13:38:55Z Fine-mapping the wheat Snn1 locus conferring sensitivity to the Parastagonospora nodorum necrotrophic effector SnTox1 using an eight founder multiparent advanced generation inter-cross population Cockram, J. Scuderi, A. Barber, T. Furuki, E. Gardner, K. Gosman, N. Kowalczyk, R. Phan, H. Rose, G. Tan, K. Oliver, Richard Mackay, I. The necrotrophic fungus Parastagonospora nodorum is an important pathogen of one of the world’s most economically important cereal crops, wheat (Triticum aestivum L.). P. nodorum produces necrotrophic protein effectors that mediate host cell death, providing nutrients for continuation of the infection process. The recent discovery of pathogen effectors has revolutionized disease resistance breeding for necrotrophic diseases in crop species, allowing often complex genetic resistance mechanisms to be broken down into constituent parts. To date, three effectors have been identified in P. nodorum. Here we use the effector, SnTox1, to screen 642 progeny from an eight-parent multiparent advanced generation inter-cross (i.e., MAGIC) population, genotyped with a 90,000-feature single-nucleotide polymorphism array. The MAGIC founders showed a range of sensitivity to SnTox1, with transgressive segregation evident in the progeny. SnTox1 sensitivity showed high heritability, with quantitative trait locus analyses fine-mapping the Snn1 locus to the short arm of chromosome 1B. In addition, a previously undescribed SnTox1 sensitivity locus was identified on the long arm of chromosome 5A, termed here QSnn.niab-5A.1. The peak single-nucleotide polymorphism for the Snn1 locus was converted to the KASP genotyping platform, providing breeders and researchers a simple and cheap diagnostic marker for allelic state at Snn1. 2015 Journal Article http://hdl.handle.net/20.500.11937/46450 10.1534/g3.115.021584 fulltext |
| spellingShingle | Cockram, J. Scuderi, A. Barber, T. Furuki, E. Gardner, K. Gosman, N. Kowalczyk, R. Phan, H. Rose, G. Tan, K. Oliver, Richard Mackay, I. Fine-mapping the wheat Snn1 locus conferring sensitivity to the Parastagonospora nodorum necrotrophic effector SnTox1 using an eight founder multiparent advanced generation inter-cross population |
| title | Fine-mapping the wheat Snn1 locus conferring sensitivity to the Parastagonospora nodorum necrotrophic effector SnTox1 using an eight founder multiparent advanced generation inter-cross population |
| title_full | Fine-mapping the wheat Snn1 locus conferring sensitivity to the Parastagonospora nodorum necrotrophic effector SnTox1 using an eight founder multiparent advanced generation inter-cross population |
| title_fullStr | Fine-mapping the wheat Snn1 locus conferring sensitivity to the Parastagonospora nodorum necrotrophic effector SnTox1 using an eight founder multiparent advanced generation inter-cross population |
| title_full_unstemmed | Fine-mapping the wheat Snn1 locus conferring sensitivity to the Parastagonospora nodorum necrotrophic effector SnTox1 using an eight founder multiparent advanced generation inter-cross population |
| title_short | Fine-mapping the wheat Snn1 locus conferring sensitivity to the Parastagonospora nodorum necrotrophic effector SnTox1 using an eight founder multiparent advanced generation inter-cross population |
| title_sort | fine-mapping the wheat snn1 locus conferring sensitivity to the parastagonospora nodorum necrotrophic effector sntox1 using an eight founder multiparent advanced generation inter-cross population |
| url | http://hdl.handle.net/20.500.11937/46450 |