Comparison of Rhinovirus Antibody Titers in Children with Asthma Exacerbations and Species-Specific Rhinovirus Infection

Background: Asthma exacerbations are associated with human rhinovirus (HRV) infections, and more severe exacerbations are associated with HRV-C. We have previously shown that the HRV-C–specific antibody response is low in healthy adult sera and that most of the antibody to HRV-C is cross-reactive wi...

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Main Authors: Iwasaki, J., Smith, W., Khoo, S., Bizzintino, J., Zhang, Guicheng, Cox, D., Laing, I., Le Souef, P., Thomas, W., Hales, B.
Format: Journal Article
Published: Mosby, Inc. 2014
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/46386
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author Iwasaki, J.
Smith, W.
Khoo, S.
Bizzintino, J.
Zhang, Guicheng
Cox, D.
Laing, I.
Le Souef, P.
Thomas, W.
Hales, B.
author_facet Iwasaki, J.
Smith, W.
Khoo, S.
Bizzintino, J.
Zhang, Guicheng
Cox, D.
Laing, I.
Le Souef, P.
Thomas, W.
Hales, B.
author_sort Iwasaki, J.
building Curtin Institutional Repository
collection Online Access
description Background: Asthma exacerbations are associated with human rhinovirus (HRV) infections, and more severe exacerbations are associated with HRV-C. We have previously shown that the HRV-C–specific antibody response is low in healthy adult sera and that most of the antibody to HRV-C is cross-reactive with HRV-A. Objectives: To compare the antibody response to each HRV species in asthmatic and nonasthmatic children in whom the type of HRV infection was known. Methods: Total and specific IgG1 binding to HRV viral capsid protein antigens of HRV-A, -B, and -C were tested in the plasma from nonasthmatic children (n = 47) and children presenting to the emergency department with asthma exacerbations (n = 96). HRV, found in most of the children at the time of their exacerbation (72%), was analyzed using molecular typing. Results: Asthmatic children had higher antibody responses to HRV. The titers specific to HRV-A, and to a lesser extent HRV-B, were higher than in nonasthmatic controls. The species-specific responses to HRV-C were markedly lower than titers to HRV-A and HRV-B in both asthmatic and nonasthmatic children (P < .001). The titers both at presentation and after convalescence were not associated with the HRV genotype detected during the exacerbation. Conclusions: The higher total anti-HRV antibody titers of asthmatic children and their higher anti–HRV-A and -B titers show their development of a heightened antiviral immune response. The low species-specific HRV-C titers found in all groups, even when the virus was found, point to a different and possibly less efficacious immune response to this species.
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spelling curtin-20.500.11937-463862017-09-13T15:57:55Z Comparison of Rhinovirus Antibody Titers in Children with Asthma Exacerbations and Species-Specific Rhinovirus Infection Iwasaki, J. Smith, W. Khoo, S. Bizzintino, J. Zhang, Guicheng Cox, D. Laing, I. Le Souef, P. Thomas, W. Hales, B. VP1 asthma exacerbation children Human rhinovirus IgG1 antibody Background: Asthma exacerbations are associated with human rhinovirus (HRV) infections, and more severe exacerbations are associated with HRV-C. We have previously shown that the HRV-C–specific antibody response is low in healthy adult sera and that most of the antibody to HRV-C is cross-reactive with HRV-A. Objectives: To compare the antibody response to each HRV species in asthmatic and nonasthmatic children in whom the type of HRV infection was known. Methods: Total and specific IgG1 binding to HRV viral capsid protein antigens of HRV-A, -B, and -C were tested in the plasma from nonasthmatic children (n = 47) and children presenting to the emergency department with asthma exacerbations (n = 96). HRV, found in most of the children at the time of their exacerbation (72%), was analyzed using molecular typing. Results: Asthmatic children had higher antibody responses to HRV. The titers specific to HRV-A, and to a lesser extent HRV-B, were higher than in nonasthmatic controls. The species-specific responses to HRV-C were markedly lower than titers to HRV-A and HRV-B in both asthmatic and nonasthmatic children (P < .001). The titers both at presentation and after convalescence were not associated with the HRV genotype detected during the exacerbation. Conclusions: The higher total anti-HRV antibody titers of asthmatic children and their higher anti–HRV-A and -B titers show their development of a heightened antiviral immune response. The low species-specific HRV-C titers found in all groups, even when the virus was found, point to a different and possibly less efficacious immune response to this species. 2014 Journal Article http://hdl.handle.net/20.500.11937/46386 10.1016/j.jaci.2014.03.014 Mosby, Inc. restricted
spellingShingle VP1
asthma exacerbation
children
Human rhinovirus
IgG1 antibody
Iwasaki, J.
Smith, W.
Khoo, S.
Bizzintino, J.
Zhang, Guicheng
Cox, D.
Laing, I.
Le Souef, P.
Thomas, W.
Hales, B.
Comparison of Rhinovirus Antibody Titers in Children with Asthma Exacerbations and Species-Specific Rhinovirus Infection
title Comparison of Rhinovirus Antibody Titers in Children with Asthma Exacerbations and Species-Specific Rhinovirus Infection
title_full Comparison of Rhinovirus Antibody Titers in Children with Asthma Exacerbations and Species-Specific Rhinovirus Infection
title_fullStr Comparison of Rhinovirus Antibody Titers in Children with Asthma Exacerbations and Species-Specific Rhinovirus Infection
title_full_unstemmed Comparison of Rhinovirus Antibody Titers in Children with Asthma Exacerbations and Species-Specific Rhinovirus Infection
title_short Comparison of Rhinovirus Antibody Titers in Children with Asthma Exacerbations and Species-Specific Rhinovirus Infection
title_sort comparison of rhinovirus antibody titers in children with asthma exacerbations and species-specific rhinovirus infection
topic VP1
asthma exacerbation
children
Human rhinovirus
IgG1 antibody
url http://hdl.handle.net/20.500.11937/46386