Isolation and Characterization of Alpaca Tetranucleotide Microsatellite Markers
Hybridisation-capture was used to create 12 unique alpaca DNA libraries each enriched for a different tetranucleotide microsatellite motif. Two hundred and forty nine microsatellites were found, of which 26 were polymorphic (motifs GGAT, GTTT and GCAC). Nine markers were fully characterised on 45 s...
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| Format: | Conference Paper |
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Association for the Advancement of Animal Breeding and Genetics
2009
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| Online Access: | http://www.aaabg.org/proceedings18/index.html http://hdl.handle.net/20.500.11937/46352 |
| _version_ | 1848757533515186176 |
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| author | Munyard, Kylie Ledger, J. Lee, C. Babra, C. Groth, David |
| author2 | Alex Safari |
| author_facet | Alex Safari Munyard, Kylie Ledger, J. Lee, C. Babra, C. Groth, David |
| author_sort | Munyard, Kylie |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Hybridisation-capture was used to create 12 unique alpaca DNA libraries each enriched for a different tetranucleotide microsatellite motif. Two hundred and forty nine microsatellites were found, of which 26 were polymorphic (motifs GGAT, GTTT and GCAC). Nine markers were fully characterised on 45 samples. Allele numbers ranged from 6 (Locus P135) to 12 (loci P149 and PCTD17). There was no evidence of linkage disequilibrium (p = 0.064 - 1) or deviation from Hardy-Weinberg equilibrium (p = 1). Polymorphic information content ranged from 0.48 to 0.82. When combined, the markers had an exclusion probability of 97.7%. These markers will be useful for parentage determination (especially if combined into a multiplex) and will add to the pool of markers available for mapping of desirous or deleterious traits in alpacas. |
| first_indexed | 2025-11-14T09:29:36Z |
| format | Conference Paper |
| id | curtin-20.500.11937-46352 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:29:36Z |
| publishDate | 2009 |
| publisher | Association for the Advancement of Animal Breeding and Genetics |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-463522022-12-09T07:12:34Z Isolation and Characterization of Alpaca Tetranucleotide Microsatellite Markers Munyard, Kylie Ledger, J. Lee, C. Babra, C. Groth, David Alex Safari Bill Pattie Barrie Restall microsatellite alpaca Hybridisation-capture was used to create 12 unique alpaca DNA libraries each enriched for a different tetranucleotide microsatellite motif. Two hundred and forty nine microsatellites were found, of which 26 were polymorphic (motifs GGAT, GTTT and GCAC). Nine markers were fully characterised on 45 samples. Allele numbers ranged from 6 (Locus P135) to 12 (loci P149 and PCTD17). There was no evidence of linkage disequilibrium (p = 0.064 - 1) or deviation from Hardy-Weinberg equilibrium (p = 1). Polymorphic information content ranged from 0.48 to 0.82. When combined, the markers had an exclusion probability of 97.7%. These markers will be useful for parentage determination (especially if combined into a multiplex) and will add to the pool of markers available for mapping of desirous or deleterious traits in alpacas. 2009 Conference Paper http://hdl.handle.net/20.500.11937/46352 http://www.aaabg.org/proceedings18/index.html Association for the Advancement of Animal Breeding and Genetics fulltext |
| spellingShingle | microsatellite alpaca Munyard, Kylie Ledger, J. Lee, C. Babra, C. Groth, David Isolation and Characterization of Alpaca Tetranucleotide Microsatellite Markers |
| title | Isolation and Characterization of Alpaca Tetranucleotide Microsatellite Markers |
| title_full | Isolation and Characterization of Alpaca Tetranucleotide Microsatellite Markers |
| title_fullStr | Isolation and Characterization of Alpaca Tetranucleotide Microsatellite Markers |
| title_full_unstemmed | Isolation and Characterization of Alpaca Tetranucleotide Microsatellite Markers |
| title_short | Isolation and Characterization of Alpaca Tetranucleotide Microsatellite Markers |
| title_sort | isolation and characterization of alpaca tetranucleotide microsatellite markers |
| topic | microsatellite alpaca |
| url | http://www.aaabg.org/proceedings18/index.html http://hdl.handle.net/20.500.11937/46352 |