Microsatellite primers for the rare endemic shrub Acacia adinophylla (Fabaceae)
Premise of the study: Microsatellite primers were developed for the rare shrub Acacia adinophylla (Fabaceae) to assess genetic diversity and its spatial structuring. Methods and Results: Shotgun sequencing on an Illumina MiSeq produced 6,372,575 reads. Using the QDD pipeline, we designed 60 primer p...
| Main Authors: | , |
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| Format: | Journal Article |
| Published: |
2016
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| Online Access: | http://purl.org/au-research/grants/arc/IC150100041 http://hdl.handle.net/20.500.11937/45792 |
| _version_ | 1848757383441940480 |
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| author | Nevill, Paul Wardell-Johnson, G. |
| author_facet | Nevill, Paul Wardell-Johnson, G. |
| author_sort | Nevill, Paul |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Premise of the study: Microsatellite primers were developed for the rare shrub Acacia adinophylla (Fabaceae) to assess genetic diversity and its spatial structuring. Methods and Results: Shotgun sequencing on an Illumina MiSeq produced 6,372,575 reads. Using the QDD pipeline, we designed 60 primer pairs, which were screened using PCR. Seventeen loci were developed, of which 12 loci were identified that were polymorphic, amplified reliably, and could be consistently scored. These loci were then screened for variation in individuals from three populations. The number of alleles observed for these 12 loci ranged from three to 18 and expected heterozygosity ranged from 0.13 to 0.85. Conclusions: These markers will enable the quantification of genetic impact of proposed mining activities on the short-range endemic Acacia adinophylla. |
| first_indexed | 2025-11-14T09:27:13Z |
| format | Journal Article |
| id | curtin-20.500.11937-45792 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:27:13Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-457922022-01-06T07:11:11Z Microsatellite primers for the rare endemic shrub Acacia adinophylla (Fabaceae) Nevill, Paul Wardell-Johnson, G. Premise of the study: Microsatellite primers were developed for the rare shrub Acacia adinophylla (Fabaceae) to assess genetic diversity and its spatial structuring. Methods and Results: Shotgun sequencing on an Illumina MiSeq produced 6,372,575 reads. Using the QDD pipeline, we designed 60 primer pairs, which were screened using PCR. Seventeen loci were developed, of which 12 loci were identified that were polymorphic, amplified reliably, and could be consistently scored. These loci were then screened for variation in individuals from three populations. The number of alleles observed for these 12 loci ranged from three to 18 and expected heterozygosity ranged from 0.13 to 0.85. Conclusions: These markers will enable the quantification of genetic impact of proposed mining activities on the short-range endemic Acacia adinophylla. 2016 Journal Article http://hdl.handle.net/20.500.11937/45792 10.3732/apps.1600084 http://purl.org/au-research/grants/arc/IC150100041 fulltext |
| spellingShingle | Nevill, Paul Wardell-Johnson, G. Microsatellite primers for the rare endemic shrub Acacia adinophylla (Fabaceae) |
| title | Microsatellite primers for the rare endemic shrub Acacia adinophylla (Fabaceae) |
| title_full | Microsatellite primers for the rare endemic shrub Acacia adinophylla (Fabaceae) |
| title_fullStr | Microsatellite primers for the rare endemic shrub Acacia adinophylla (Fabaceae) |
| title_full_unstemmed | Microsatellite primers for the rare endemic shrub Acacia adinophylla (Fabaceae) |
| title_short | Microsatellite primers for the rare endemic shrub Acacia adinophylla (Fabaceae) |
| title_sort | microsatellite primers for the rare endemic shrub acacia adinophylla (fabaceae) |
| url | http://purl.org/au-research/grants/arc/IC150100041 http://hdl.handle.net/20.500.11937/45792 |