Reduced cytotoxicity of silver ions to mammalian cells at high concentration due to the formation of silver chloride
Silver-containing antimicrobial agents are used in various medical products. However, their toxicity to mammalian cells has not been sufficiently evaluated. Numerous studies have unveiled evidence of significant antimicrobial properties associated with Ag ions. In cell culture media or human body fl...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
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Pergamon
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/45701 |
| _version_ | 1848757358430257152 |
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| author | Zhang, S. Du, C. Wang, Z. Han, X. Zhang, Kun Liu, Lihong |
| author_facet | Zhang, S. Du, C. Wang, Z. Han, X. Zhang, Kun Liu, Lihong |
| author_sort | Zhang, S. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Silver-containing antimicrobial agents are used in various medical products. However, their toxicity to mammalian cells has not been sufficiently evaluated. Numerous studies have unveiled evidence of significant antimicrobial properties associated with Ag ions. In cell culture media or human body fluids, the free Ag+ has rich opportunities to complex with Cl-. Surprisingly, studies on the toxicity of solid form AgCl(s) to mammalian cells are quite limited. In this study, we evaluated the cytotoxicity of Ag ions and silver chloride colloids on red blood cells and human mesenchymal stem cells (hMSCs). The adverse effects of silver chloride on red blood cells and hMSC were viewed by SEM and LIVE/DEAD viability staining, respectively. Among different tested chemical forms of silver, AgCl was identified to be the least cytotoxic. Moreover, a decline in the cytotoxicity of AgCl at significantly high concentrations was observed. We attributed the reduced cytotoxicity to aggregated AgCl which limited the bioavailability of free Ag+ ions. |
| first_indexed | 2025-11-14T09:26:49Z |
| format | Journal Article |
| id | curtin-20.500.11937-45701 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:26:49Z |
| publishDate | 2013 |
| publisher | Pergamon |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-457012019-02-19T04:27:24Z Reduced cytotoxicity of silver ions to mammalian cells at high concentration due to the formation of silver chloride Zhang, S. Du, C. Wang, Z. Han, X. Zhang, Kun Liu, Lihong Speciation Silver chloride Stem cells Cytotoxicity Hemolysis Silver Silver-containing antimicrobial agents are used in various medical products. However, their toxicity to mammalian cells has not been sufficiently evaluated. Numerous studies have unveiled evidence of significant antimicrobial properties associated with Ag ions. In cell culture media or human body fluids, the free Ag+ has rich opportunities to complex with Cl-. Surprisingly, studies on the toxicity of solid form AgCl(s) to mammalian cells are quite limited. In this study, we evaluated the cytotoxicity of Ag ions and silver chloride colloids on red blood cells and human mesenchymal stem cells (hMSCs). The adverse effects of silver chloride on red blood cells and hMSC were viewed by SEM and LIVE/DEAD viability staining, respectively. Among different tested chemical forms of silver, AgCl was identified to be the least cytotoxic. Moreover, a decline in the cytotoxicity of AgCl at significantly high concentrations was observed. We attributed the reduced cytotoxicity to aggregated AgCl which limited the bioavailability of free Ag+ ions. 2013 Journal Article http://hdl.handle.net/20.500.11937/45701 10.1016/j.tiv.2012.12.003 Pergamon fulltext |
| spellingShingle | Speciation Silver chloride Stem cells Cytotoxicity Hemolysis Silver Zhang, S. Du, C. Wang, Z. Han, X. Zhang, Kun Liu, Lihong Reduced cytotoxicity of silver ions to mammalian cells at high concentration due to the formation of silver chloride |
| title | Reduced cytotoxicity of silver ions to mammalian cells at high concentration due to the formation of silver chloride |
| title_full | Reduced cytotoxicity of silver ions to mammalian cells at high concentration due to the formation of silver chloride |
| title_fullStr | Reduced cytotoxicity of silver ions to mammalian cells at high concentration due to the formation of silver chloride |
| title_full_unstemmed | Reduced cytotoxicity of silver ions to mammalian cells at high concentration due to the formation of silver chloride |
| title_short | Reduced cytotoxicity of silver ions to mammalian cells at high concentration due to the formation of silver chloride |
| title_sort | reduced cytotoxicity of silver ions to mammalian cells at high concentration due to the formation of silver chloride |
| topic | Speciation Silver chloride Stem cells Cytotoxicity Hemolysis Silver |
| url | http://hdl.handle.net/20.500.11937/45701 |