Isolation of 15 new polymorphic microsatellite markers from the blue-spine unicornfish Naso unicornis
The blue-spine unicornfish Naso unicornis is a widely distributed reef herbivore that is highly prized in tropical Indo-Pacific fisheries. Appropriate management for N. unicornis and other exploited reef fishes requires detailed knowledge of larval migrant exchange between isolated adult meta-popula...
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| Format: | Journal Article |
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Springer
2010
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| Online Access: | http://hdl.handle.net/20.500.11937/45327 |
| _version_ | 1848757251992453120 |
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| author | Horne, J. McIlwain, Jennifer van Herwerden, L. |
| author_facet | Horne, J. McIlwain, Jennifer van Herwerden, L. |
| author_sort | Horne, J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The blue-spine unicornfish Naso unicornis is a widely distributed reef herbivore that is highly prized in tropical Indo-Pacific fisheries. Appropriate management for N. unicornis and other exploited reef fishes requires detailed knowledge of larval migrant exchange between isolated adult meta-populations and an understanding of recruitment patterns at both local and larger geographic scales. To this end, we have developed 15 microsatellite loci to evaluate levels larval connectivity and detect genetic patterns relevant to demographic processes in this species. Microsatellites were isolated from total genomic DNA using biotinylated probes and magnetic bead capture. We screened these loci against 90 individuals sampled from Guam in the tropical West Pacific. Loci contained 5–23 alleles (mean = 15.7) and had a mean observed and expected heterozygosity of 0.66 and 0.82, respectively. One locus, which did not conform to the expectations of Hardy–Weinberg equilibrium, is probably under selection. Four others are probably confounded by the presence of null alleles. |
| first_indexed | 2025-11-14T09:25:08Z |
| format | Journal Article |
| id | curtin-20.500.11937-45327 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:25:08Z |
| publishDate | 2010 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-453272017-09-13T14:20:53Z Isolation of 15 new polymorphic microsatellite markers from the blue-spine unicornfish Naso unicornis Horne, J. McIlwain, Jennifer van Herwerden, L. Fisheries Micronesia Reef fishes Naso unicornis Indo-Pacific The blue-spine unicornfish Naso unicornis is a widely distributed reef herbivore that is highly prized in tropical Indo-Pacific fisheries. Appropriate management for N. unicornis and other exploited reef fishes requires detailed knowledge of larval migrant exchange between isolated adult meta-populations and an understanding of recruitment patterns at both local and larger geographic scales. To this end, we have developed 15 microsatellite loci to evaluate levels larval connectivity and detect genetic patterns relevant to demographic processes in this species. Microsatellites were isolated from total genomic DNA using biotinylated probes and magnetic bead capture. We screened these loci against 90 individuals sampled from Guam in the tropical West Pacific. Loci contained 5–23 alleles (mean = 15.7) and had a mean observed and expected heterozygosity of 0.66 and 0.82, respectively. One locus, which did not conform to the expectations of Hardy–Weinberg equilibrium, is probably under selection. Four others are probably confounded by the presence of null alleles. 2010 Journal Article http://hdl.handle.net/20.500.11937/45327 10.1007/s12686-009-9129-1 Springer restricted |
| spellingShingle | Fisheries Micronesia Reef fishes Naso unicornis Indo-Pacific Horne, J. McIlwain, Jennifer van Herwerden, L. Isolation of 15 new polymorphic microsatellite markers from the blue-spine unicornfish Naso unicornis |
| title | Isolation of 15 new polymorphic microsatellite markers from the blue-spine unicornfish Naso unicornis |
| title_full | Isolation of 15 new polymorphic microsatellite markers from the blue-spine unicornfish Naso unicornis |
| title_fullStr | Isolation of 15 new polymorphic microsatellite markers from the blue-spine unicornfish Naso unicornis |
| title_full_unstemmed | Isolation of 15 new polymorphic microsatellite markers from the blue-spine unicornfish Naso unicornis |
| title_short | Isolation of 15 new polymorphic microsatellite markers from the blue-spine unicornfish Naso unicornis |
| title_sort | isolation of 15 new polymorphic microsatellite markers from the blue-spine unicornfish naso unicornis |
| topic | Fisheries Micronesia Reef fishes Naso unicornis Indo-Pacific |
| url | http://hdl.handle.net/20.500.11937/45327 |