Mesoporous silicon photonic crystal microparticles: Towards single-cell optical biosensors
In this paper we demonstrate the possibility of modifying porous silicon (PSi) particles with surface chemistry and recognition molecules (antibodies) such that these devices could potentially be used for single-cell identification or sensing. This is achieved by modifying PSi Rugate filters via hyd...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
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R S C Publications
2011
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| Online Access: | http://hdl.handle.net/20.500.11937/4499 |
| _version_ | 1848744532772913152 |
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| author | Guan, B. Magenau, A. Kilian, K. Ciampi, Simone Gaus, K. Reece, P. Gooding, J. |
| author_facet | Guan, B. Magenau, A. Kilian, K. Ciampi, Simone Gaus, K. Reece, P. Gooding, J. |
| author_sort | Guan, B. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | In this paper we demonstrate the possibility of modifying porous silicon (PSi) particles with surface chemistry and recognition molecules (antibodies) such that these devices could potentially be used for single-cell identification or sensing. This is achieved by modifying PSi Rugate filters via hydrosilylation with surface chemistry that serves firstly, to protect the silicon surfaces from oxidation; secondly, renders the surfaces resistant to nonspecific adsorption of proteins and cells and thirdly, allows further functionality to be added such as the coupling of antibodies. The surface chemistry remained unchanged after sonication of the PSi to form PSi microparticles. The ability to monitor the spectroscopic properties of microparticles, and shifts in the optical signature due to changes in the refractive index of the material within the pore space, is demonstrated. The particles are shown to remain stable in physiological buffers and human blood for longer than one week. Finally, the modification of the PSi particles with functional antibodies is achieved. © 2011 The Royal Society of Chemistry. |
| first_indexed | 2025-11-14T06:02:58Z |
| format | Journal Article |
| id | curtin-20.500.11937-4499 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:02:58Z |
| publishDate | 2011 |
| publisher | R S C Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-44992018-03-29T09:05:25Z Mesoporous silicon photonic crystal microparticles: Towards single-cell optical biosensors Guan, B. Magenau, A. Kilian, K. Ciampi, Simone Gaus, K. Reece, P. Gooding, J. In this paper we demonstrate the possibility of modifying porous silicon (PSi) particles with surface chemistry and recognition molecules (antibodies) such that these devices could potentially be used for single-cell identification or sensing. This is achieved by modifying PSi Rugate filters via hydrosilylation with surface chemistry that serves firstly, to protect the silicon surfaces from oxidation; secondly, renders the surfaces resistant to nonspecific adsorption of proteins and cells and thirdly, allows further functionality to be added such as the coupling of antibodies. The surface chemistry remained unchanged after sonication of the PSi to form PSi microparticles. The ability to monitor the spectroscopic properties of microparticles, and shifts in the optical signature due to changes in the refractive index of the material within the pore space, is demonstrated. The particles are shown to remain stable in physiological buffers and human blood for longer than one week. Finally, the modification of the PSi particles with functional antibodies is achieved. © 2011 The Royal Society of Chemistry. 2011 Journal Article http://hdl.handle.net/20.500.11937/4499 10.1039/c005340f R S C Publications restricted |
| spellingShingle | Guan, B. Magenau, A. Kilian, K. Ciampi, Simone Gaus, K. Reece, P. Gooding, J. Mesoporous silicon photonic crystal microparticles: Towards single-cell optical biosensors |
| title | Mesoporous silicon photonic crystal microparticles: Towards single-cell optical biosensors |
| title_full | Mesoporous silicon photonic crystal microparticles: Towards single-cell optical biosensors |
| title_fullStr | Mesoporous silicon photonic crystal microparticles: Towards single-cell optical biosensors |
| title_full_unstemmed | Mesoporous silicon photonic crystal microparticles: Towards single-cell optical biosensors |
| title_short | Mesoporous silicon photonic crystal microparticles: Towards single-cell optical biosensors |
| title_sort | mesoporous silicon photonic crystal microparticles: towards single-cell optical biosensors |
| url | http://hdl.handle.net/20.500.11937/4499 |