Confirmed case of encephalitis caused by Murray Valley encephalitis virus infection in a horse

A five year old Australian stock horse in Monto, Queensland, developed neurological signs and was euthanized after a six day course of illness. Histological examination of the brain and spinal cord revealed moderate to severe, subacute, non-suppurative encephalomyelitis. Sections of spinal cord stai...

Full description

Bibliographic Details
Main Authors: Gordon, A., Marbach, C., Oakey, J., Edmunds, G., Condon, K., Diviney, Sinead, Williams, David, Bingham, J.
Format: Journal Article
Published: SAGE Publications 2012
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/44892
_version_ 1848757130821107712
author Gordon, A.
Marbach, C.
Oakey, J.
Edmunds, G.
Condon, K.
Diviney, Sinead
Williams, David
Bingham, J.
author_facet Gordon, A.
Marbach, C.
Oakey, J.
Edmunds, G.
Condon, K.
Diviney, Sinead
Williams, David
Bingham, J.
author_sort Gordon, A.
building Curtin Institutional Repository
collection Online Access
description A five year old Australian stock horse in Monto, Queensland, developed neurological signs and was euthanized after a six day course of illness. Histological examination of the brain and spinal cord revealed moderate to severe, subacute, non-suppurative encephalomyelitis. Sections of spinal cord stained positively in immunohistochemistry with a flavivirus-specific monoclonal antibody. Reverse transcription-polymerase chain reaction assay targeting the envelope gene of flavivirus yielded positive results from brain, spinal cord, cerebrospinal fluid and facial nerve. A flavivirus was isolated from the cerebrum and spinal cord. Nucleotide sequences obtained from amplicons from both tissues and virus isolated in cell culture were compared with those in GenBank, and had 96-98% identity with Murray Valley encephalitis virus. The partial envelope gene sequence of the viral isolate clustered into Genotype 1, and was most closely related to a previous Queensland isolate. This is the first confirmed case of naturally-occurring equine encephalitis attributable to Murray Valley encephalitis virus infection.
first_indexed 2025-11-14T09:23:12Z
format Journal Article
id curtin-20.500.11937-44892
institution Curtin University Malaysia
institution_category Local University
last_indexed 2025-11-14T09:23:12Z
publishDate 2012
publisher SAGE Publications
recordtype eprints
repository_type Digital Repository
spelling curtin-20.500.11937-448922017-09-13T14:18:23Z Confirmed case of encephalitis caused by Murray Valley encephalitis virus infection in a horse Gordon, A. Marbach, C. Oakey, J. Edmunds, G. Condon, K. Diviney, Sinead Williams, David Bingham, J. Murray Valley encephalitis virus flavivirus horses Encephalitis A five year old Australian stock horse in Monto, Queensland, developed neurological signs and was euthanized after a six day course of illness. Histological examination of the brain and spinal cord revealed moderate to severe, subacute, non-suppurative encephalomyelitis. Sections of spinal cord stained positively in immunohistochemistry with a flavivirus-specific monoclonal antibody. Reverse transcription-polymerase chain reaction assay targeting the envelope gene of flavivirus yielded positive results from brain, spinal cord, cerebrospinal fluid and facial nerve. A flavivirus was isolated from the cerebrum and spinal cord. Nucleotide sequences obtained from amplicons from both tissues and virus isolated in cell culture were compared with those in GenBank, and had 96-98% identity with Murray Valley encephalitis virus. The partial envelope gene sequence of the viral isolate clustered into Genotype 1, and was most closely related to a previous Queensland isolate. This is the first confirmed case of naturally-occurring equine encephalitis attributable to Murray Valley encephalitis virus infection. 2012 Journal Article http://hdl.handle.net/20.500.11937/44892 10.1177/1040638711433325 SAGE Publications fulltext
spellingShingle Murray Valley encephalitis virus
flavivirus
horses
Encephalitis
Gordon, A.
Marbach, C.
Oakey, J.
Edmunds, G.
Condon, K.
Diviney, Sinead
Williams, David
Bingham, J.
Confirmed case of encephalitis caused by Murray Valley encephalitis virus infection in a horse
title Confirmed case of encephalitis caused by Murray Valley encephalitis virus infection in a horse
title_full Confirmed case of encephalitis caused by Murray Valley encephalitis virus infection in a horse
title_fullStr Confirmed case of encephalitis caused by Murray Valley encephalitis virus infection in a horse
title_full_unstemmed Confirmed case of encephalitis caused by Murray Valley encephalitis virus infection in a horse
title_short Confirmed case of encephalitis caused by Murray Valley encephalitis virus infection in a horse
title_sort confirmed case of encephalitis caused by murray valley encephalitis virus infection in a horse
topic Murray Valley encephalitis virus
flavivirus
horses
Encephalitis
url http://hdl.handle.net/20.500.11937/44892