A regulatory toolbox of MiniPromoters to drive selective expression in the brain

The Pleiades Promoter Project integrates genomewide bioinformatics with large-scale knockin mouse production and histological examination of expression patterns to develop MiniPromoters and related tools designed to study and treat the brain by directed gene expression. Genes with brain expression p...

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Main Authors:	Portales-Casamar, E., Swanson, D., Liu, L., De Leeuw, C., Banks, K., Ho Sui, S., Fulton, D., Ali, J., Amirabbasi, M., Arenillas, D., Babyak, N., Black, S., Bonaguro, R., Brauer, E., Candido, T., Castellarin, M., Chen, J., Chen, Y., Cheng, J., Chopra, V., Docking, T., Dreolini, L., D'Souza, C., Flynn, E., Glenn, R., Hatakka, K., Hearty, T., Imanian, B., Jiang, S., Khorasan-zadeh, S., Komljenovic, I., Laprise, S., Liao, N., Lim, J., Lithwick, S., Liu, F., Liu, J., Lu, M., McConechy, M., McLeod, A., Milisavljevic, M., Mis, J., O'Connor, K., Palma, B., Palmquist, D., Schmouth, J., Swanson, M., Tam, B., Ticoll, A., Turner, J., Varhol, Richard, Vermeulen, J., Watkins, R., Wilson, G., Wong, B., Wong, S., Wong, T., Yang, G., Ypsilanti, A., Jones, S., Holt, R., Goldowitz, D., Wasserman, W., Simpson, E.
Format:	Journal Article
Published:	National Academy of Sciences 2010
Online Access:	http://hdl.handle.net/20.500.11937/44520

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author	Portales-Casamar, E. Swanson, D. Liu, L. De Leeuw, C. Banks, K. Ho Sui, S. Fulton, D. Ali, J. Amirabbasi, M. Arenillas, D. Babyak, N. Black, S. Bonaguro, R. Brauer, E. Candido, T. Castellarin, M. Chen, J. Chen, Y. Cheng, J. Chopra, V. Docking, T. Dreolini, L. D'Souza, C. Flynn, E. Glenn, R. Hatakka, K. Hearty, T. Imanian, B. Jiang, S. Khorasan-zadeh, S. Komljenovic, I. Laprise, S. Liao, N. Lim, J. Lithwick, S. Liu, F. Liu, J. Lu, M. McConechy, M. McLeod, A. Milisavljevic, M. Mis, J. O'Connor, K. Palma, B. Palmquist, D. Schmouth, J. Swanson, M. Tam, B. Ticoll, A. Turner, J. Varhol, Richard Vermeulen, J. Watkins, R. Wilson, G. Wong, B. Wong, S. Wong, T. Yang, G. Ypsilanti, A. Jones, S. Holt, R. Goldowitz, D. Wasserman, W. Simpson, E.
author_facet	Portales-Casamar, E. Swanson, D. Liu, L. De Leeuw, C. Banks, K. Ho Sui, S. Fulton, D. Ali, J. Amirabbasi, M. Arenillas, D. Babyak, N. Black, S. Bonaguro, R. Brauer, E. Candido, T. Castellarin, M. Chen, J. Chen, Y. Cheng, J. Chopra, V. Docking, T. Dreolini, L. D'Souza, C. Flynn, E. Glenn, R. Hatakka, K. Hearty, T. Imanian, B. Jiang, S. Khorasan-zadeh, S. Komljenovic, I. Laprise, S. Liao, N. Lim, J. Lithwick, S. Liu, F. Liu, J. Lu, M. McConechy, M. McLeod, A. Milisavljevic, M. Mis, J. O'Connor, K. Palma, B. Palmquist, D. Schmouth, J. Swanson, M. Tam, B. Ticoll, A. Turner, J. Varhol, Richard Vermeulen, J. Watkins, R. Wilson, G. Wong, B. Wong, S. Wong, T. Yang, G. Ypsilanti, A. Jones, S. Holt, R. Goldowitz, D. Wasserman, W. Simpson, E.
author_sort	Portales-Casamar, E.
building	Curtin Institutional Repository
collection	Online Access
description	The Pleiades Promoter Project integrates genomewide bioinformatics with large-scale knockin mouse production and histological examination of expression patterns to develop MiniPromoters and related tools designed to study and treat the brain by directed gene expression. Genes with brain expression patterns of interest are subjected to bioinformatic analysis to delineate candidate regulatory regions, which are then incorporated into a panel of compact human MiniPromoters to drive expression to brain regions and cell types of interest. Using single-copy, homologous-recombination "knockins" in embryonic stem cells, each MiniPromoter reporter is integrated immediately 5' of the Hprt locus in the mouse genome. MiniPromoter expression profiles are characterized in differentiation assays of the transgenic cells or inmouse brains following transgenic mouse production. Histological examination of adult brains, eyes, and spinal cords for reporter gene activity is coupled to costaining with cell-type - specific markers to define expression. The publicly available Pleiades MiniPromoter Project is a key resource to facilitate research on brain development and therapies.
first_indexed	2025-11-14T09:21:32Z
format	Journal Article
id	curtin-20.500.11937-44520
institution	Curtin University Malaysia
institution_category	Local University
last_indexed	2025-11-14T09:21:32Z
publishDate	2010
publisher	National Academy of Sciences
recordtype	eprints
repository_type	Digital Repository
spelling	curtin-20.500.11937-445202023-02-22T06:24:22Z A regulatory toolbox of MiniPromoters to drive selective expression in the brain Portales-Casamar, E. Swanson, D. Liu, L. De Leeuw, C. Banks, K. Ho Sui, S. Fulton, D. Ali, J. Amirabbasi, M. Arenillas, D. Babyak, N. Black, S. Bonaguro, R. Brauer, E. Candido, T. Castellarin, M. Chen, J. Chen, Y. Cheng, J. Chopra, V. Docking, T. Dreolini, L. D'Souza, C. Flynn, E. Glenn, R. Hatakka, K. Hearty, T. Imanian, B. Jiang, S. Khorasan-zadeh, S. Komljenovic, I. Laprise, S. Liao, N. Lim, J. Lithwick, S. Liu, F. Liu, J. Lu, M. McConechy, M. McLeod, A. Milisavljevic, M. Mis, J. O'Connor, K. Palma, B. Palmquist, D. Schmouth, J. Swanson, M. Tam, B. Ticoll, A. Turner, J. Varhol, Richard Vermeulen, J. Watkins, R. Wilson, G. Wong, B. Wong, S. Wong, T. Yang, G. Ypsilanti, A. Jones, S. Holt, R. Goldowitz, D. Wasserman, W. Simpson, E. The Pleiades Promoter Project integrates genomewide bioinformatics with large-scale knockin mouse production and histological examination of expression patterns to develop MiniPromoters and related tools designed to study and treat the brain by directed gene expression. Genes with brain expression patterns of interest are subjected to bioinformatic analysis to delineate candidate regulatory regions, which are then incorporated into a panel of compact human MiniPromoters to drive expression to brain regions and cell types of interest. Using single-copy, homologous-recombination "knockins" in embryonic stem cells, each MiniPromoter reporter is integrated immediately 5' of the Hprt locus in the mouse genome. MiniPromoter expression profiles are characterized in differentiation assays of the transgenic cells or inmouse brains following transgenic mouse production. Histological examination of adult brains, eyes, and spinal cords for reporter gene activity is coupled to costaining with cell-type - specific markers to define expression. The publicly available Pleiades MiniPromoter Project is a key resource to facilitate research on brain development and therapies. 2010 Journal Article http://hdl.handle.net/20.500.11937/44520 10.1073/pnas.1009158107 National Academy of Sciences unknown
spellingShingle	Portales-Casamar, E. Swanson, D. Liu, L. De Leeuw, C. Banks, K. Ho Sui, S. Fulton, D. Ali, J. Amirabbasi, M. Arenillas, D. Babyak, N. Black, S. Bonaguro, R. Brauer, E. Candido, T. Castellarin, M. Chen, J. Chen, Y. Cheng, J. Chopra, V. Docking, T. Dreolini, L. D'Souza, C. Flynn, E. Glenn, R. Hatakka, K. Hearty, T. Imanian, B. Jiang, S. Khorasan-zadeh, S. Komljenovic, I. Laprise, S. Liao, N. Lim, J. Lithwick, S. Liu, F. Liu, J. Lu, M. McConechy, M. McLeod, A. Milisavljevic, M. Mis, J. O'Connor, K. Palma, B. Palmquist, D. Schmouth, J. Swanson, M. Tam, B. Ticoll, A. Turner, J. Varhol, Richard Vermeulen, J. Watkins, R. Wilson, G. Wong, B. Wong, S. Wong, T. Yang, G. Ypsilanti, A. Jones, S. Holt, R. Goldowitz, D. Wasserman, W. Simpson, E. A regulatory toolbox of MiniPromoters to drive selective expression in the brain
title	A regulatory toolbox of MiniPromoters to drive selective expression in the brain
title_full	A regulatory toolbox of MiniPromoters to drive selective expression in the brain
title_fullStr	A regulatory toolbox of MiniPromoters to drive selective expression in the brain
title_full_unstemmed	A regulatory toolbox of MiniPromoters to drive selective expression in the brain
title_short	A regulatory toolbox of MiniPromoters to drive selective expression in the brain
title_sort	regulatory toolbox of minipromoters to drive selective expression in the brain
url	http://hdl.handle.net/20.500.11937/44520

A regulatory toolbox of MiniPromoters to drive selective expression in the brain

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