Characterisation of polymorphic microsatellite markers in the widespread Australian seagrass, Posidonia Australis Hook. F. (Posidoniaceae), with cross-amplification in the sympatric P. Sinuosa
We developed 10 polymorphic microsatellite markers in the Australian seagrass Posidonia australis Hook. f. Markers were screened for their ability to detect within- and among-population genetic structure and variation. The markers showed a range in levels of polymorphism from fixed differences betwe...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Published: |
Springer
2009
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| Online Access: | http://hdl.handle.net/20.500.11937/44397 |
| _version_ | 1848756989043146752 |
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| author | Sinclair, E. Anthony, J. Coupland, G. Waycott, M. Barrett, M. Barrett, R. Cambridge, M. Wallace, M. Dixon, Kingsley Krauss, S. Kendrick, G. |
| author_facet | Sinclair, E. Anthony, J. Coupland, G. Waycott, M. Barrett, M. Barrett, R. Cambridge, M. Wallace, M. Dixon, Kingsley Krauss, S. Kendrick, G. |
| author_sort | Sinclair, E. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | We developed 10 polymorphic microsatellite markers in the Australian seagrass Posidonia australis Hook. f. Markers were screened for their ability to detect within- and among-population genetic structure and variation. The markers showed a range in levels of polymorphism from fixed differences between the two sampled seagrass meadows to high levels of heterozygosity. These markers will be used to estimate gene flow across the species range, characterise the mating system through paternity analysis and pollen dispersal, characterise the nature and extent of clonality, and determine the genetic differentiation of local seagrass meadows to provide information on where to source local genetic provenance material for seagrass restoration projects. Seven of the 10 loci also amplified in the sympatric P. sinuosa and will be useful in future studies in population genetics and hybridisation. © Springer Science+Business Media B.V. 2009. |
| first_indexed | 2025-11-14T09:20:57Z |
| format | Journal Article |
| id | curtin-20.500.11937-44397 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:20:57Z |
| publishDate | 2009 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-443972017-09-13T14:11:35Z Characterisation of polymorphic microsatellite markers in the widespread Australian seagrass, Posidonia Australis Hook. F. (Posidoniaceae), with cross-amplification in the sympatric P. Sinuosa Sinclair, E. Anthony, J. Coupland, G. Waycott, M. Barrett, M. Barrett, R. Cambridge, M. Wallace, M. Dixon, Kingsley Krauss, S. Kendrick, G. We developed 10 polymorphic microsatellite markers in the Australian seagrass Posidonia australis Hook. f. Markers were screened for their ability to detect within- and among-population genetic structure and variation. The markers showed a range in levels of polymorphism from fixed differences between the two sampled seagrass meadows to high levels of heterozygosity. These markers will be used to estimate gene flow across the species range, characterise the mating system through paternity analysis and pollen dispersal, characterise the nature and extent of clonality, and determine the genetic differentiation of local seagrass meadows to provide information on where to source local genetic provenance material for seagrass restoration projects. Seven of the 10 loci also amplified in the sympatric P. sinuosa and will be useful in future studies in population genetics and hybridisation. © Springer Science+Business Media B.V. 2009. 2009 Journal Article http://hdl.handle.net/20.500.11937/44397 10.1007/s12686-009-9067-y Springer restricted |
| spellingShingle | Sinclair, E. Anthony, J. Coupland, G. Waycott, M. Barrett, M. Barrett, R. Cambridge, M. Wallace, M. Dixon, Kingsley Krauss, S. Kendrick, G. Characterisation of polymorphic microsatellite markers in the widespread Australian seagrass, Posidonia Australis Hook. F. (Posidoniaceae), with cross-amplification in the sympatric P. Sinuosa |
| title | Characterisation of polymorphic microsatellite markers in the widespread Australian seagrass, Posidonia Australis Hook. F. (Posidoniaceae), with cross-amplification in the sympatric P. Sinuosa |
| title_full | Characterisation of polymorphic microsatellite markers in the widespread Australian seagrass, Posidonia Australis Hook. F. (Posidoniaceae), with cross-amplification in the sympatric P. Sinuosa |
| title_fullStr | Characterisation of polymorphic microsatellite markers in the widespread Australian seagrass, Posidonia Australis Hook. F. (Posidoniaceae), with cross-amplification in the sympatric P. Sinuosa |
| title_full_unstemmed | Characterisation of polymorphic microsatellite markers in the widespread Australian seagrass, Posidonia Australis Hook. F. (Posidoniaceae), with cross-amplification in the sympatric P. Sinuosa |
| title_short | Characterisation of polymorphic microsatellite markers in the widespread Australian seagrass, Posidonia Australis Hook. F. (Posidoniaceae), with cross-amplification in the sympatric P. Sinuosa |
| title_sort | characterisation of polymorphic microsatellite markers in the widespread australian seagrass, posidonia australis hook. f. (posidoniaceae), with cross-amplification in the sympatric p. sinuosa |
| url | http://hdl.handle.net/20.500.11937/44397 |