DNA Extraction from Fossil Eggshell
Avian eggshell fragments recovered from both paleontological and archaeological deposits contain a cache of well-preserved ancient DNA. Here, we describe an extraction protocol that has been optimized to maximize the recovery of ancient DNA from fossil eggshell and minimize the co-purification of PC...
| Main Authors: | , |
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| Format: | Book Chapter |
| Published: |
Humana Press
2012
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| Online Access: | http://hdl.handle.net/20.500.11937/44107 |
| _version_ | 1848756902883753984 |
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| author | Oskam, C. Bunce, Michael |
| author_facet | Oskam, C. Bunce, Michael |
| author_sort | Oskam, C. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Avian eggshell fragments recovered from both paleontological and archaeological deposits contain a cache of well-preserved ancient DNA. Here, we describe an extraction protocol that has been optimized to maximize the recovery of ancient DNA from fossil eggshell and minimize the co-purification of PCR inhibitors. In this method, fossil eggshell fragments are powdered, then digested and heated to release DNA from the calcite matrix. The digest then undergoes a concentration step before purification and washing using silica columns. The method has been used to recover aDNA from the eggshell of many avian species including moa, elephant birds, and emu, up to 19,000 years old. |
| first_indexed | 2025-11-14T09:19:35Z |
| format | Book Chapter |
| id | curtin-20.500.11937-44107 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:19:35Z |
| publishDate | 2012 |
| publisher | Humana Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-441072017-09-13T14:03:55Z DNA Extraction from Fossil Eggshell Oskam, C. Bunce, Michael Avian eggshell fragments recovered from both paleontological and archaeological deposits contain a cache of well-preserved ancient DNA. Here, we describe an extraction protocol that has been optimized to maximize the recovery of ancient DNA from fossil eggshell and minimize the co-purification of PCR inhibitors. In this method, fossil eggshell fragments are powdered, then digested and heated to release DNA from the calcite matrix. The digest then undergoes a concentration step before purification and washing using silica columns. The method has been used to recover aDNA from the eggshell of many avian species including moa, elephant birds, and emu, up to 19,000 years old. 2012 Book Chapter http://hdl.handle.net/20.500.11937/44107 10.1007/978-1-61779-516-9_9 Humana Press restricted |
| spellingShingle | Oskam, C. Bunce, Michael DNA Extraction from Fossil Eggshell |
| title | DNA Extraction from Fossil Eggshell |
| title_full | DNA Extraction from Fossil Eggshell |
| title_fullStr | DNA Extraction from Fossil Eggshell |
| title_full_unstemmed | DNA Extraction from Fossil Eggshell |
| title_short | DNA Extraction from Fossil Eggshell |
| title_sort | dna extraction from fossil eggshell |
| url | http://hdl.handle.net/20.500.11937/44107 |