Stripping voltammetric detection of insulin at liquid–liquid microinterfaces in the presence of bovine albumin
Electrochemistry at the interface between two immiscible electrolyte solutions (ITIES) provides a platform for label-free detection of biomolecules. In this study, adsorptive stripping voltammetry (AdSV) was implemented at an array of microscale ITIES for the detection of the antidiabetic hormone in...
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| Format: | Journal Article |
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Royal Society of Chemistry
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/43997 |
| _version_ | 1848756871403405312 |
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| author | O'Sullivan, S. De Eulate, E. Yuen, Y. Helmerhorst, Erik Arrigan, Damien |
| author_facet | O'Sullivan, S. De Eulate, E. Yuen, Y. Helmerhorst, Erik Arrigan, Damien |
| author_sort | O'Sullivan, S. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Electrochemistry at the interface between two immiscible electrolyte solutions (ITIES) provides a platform for label-free detection of biomolecules. In this study, adsorptive stripping voltammetry (AdSV) was implemented at an array of microscale ITIES for the detection of the antidiabetic hormone insulin. By exploiting the potential-controlled adsorption of insulin at the ITIES, insulin was detected at 10 nM via subsequent voltammetric desorption. This is the lowest detected concentration reported to-date for a protein by electrochemistry at the ITIES. Surface coverage calculations indicate that between 0.1 and 1 monolayer of insulin forms at the interface over the 10 – 1000 nM concentration range of the hormone. In a step toward assessment of selectivity, the optimum adsorption potentials for insulin and albumin were determined to be 0.900 V and 0.975 V, respectively. When present in an aqueous mixture with albumin, insulin was detected by tuning the adsorption potential to 0.9 V, albeit with reduced sensitivity. This provides the first example of selective detection of one protein in the presence of another by exploiting optimal adsorption potentials. The results presented here provide a route to the improvement of detection limits and achievement of selectivity for protein detection by electrochemistry at the ITIES. |
| first_indexed | 2025-11-14T09:19:05Z |
| format | Journal Article |
| id | curtin-20.500.11937-43997 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:19:05Z |
| publishDate | 2013 |
| publisher | Royal Society of Chemistry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-439972017-09-13T14:03:54Z Stripping voltammetric detection of insulin at liquid–liquid microinterfaces in the presence of bovine albumin O'Sullivan, S. De Eulate, E. Yuen, Y. Helmerhorst, Erik Arrigan, Damien adsorptive stripping voltammetry albumin insulin ITIES liquid – liquid interface Electrochemistry at the interface between two immiscible electrolyte solutions (ITIES) provides a platform for label-free detection of biomolecules. In this study, adsorptive stripping voltammetry (AdSV) was implemented at an array of microscale ITIES for the detection of the antidiabetic hormone insulin. By exploiting the potential-controlled adsorption of insulin at the ITIES, insulin was detected at 10 nM via subsequent voltammetric desorption. This is the lowest detected concentration reported to-date for a protein by electrochemistry at the ITIES. Surface coverage calculations indicate that between 0.1 and 1 monolayer of insulin forms at the interface over the 10 – 1000 nM concentration range of the hormone. In a step toward assessment of selectivity, the optimum adsorption potentials for insulin and albumin were determined to be 0.900 V and 0.975 V, respectively. When present in an aqueous mixture with albumin, insulin was detected by tuning the adsorption potential to 0.9 V, albeit with reduced sensitivity. This provides the first example of selective detection of one protein in the presence of another by exploiting optimal adsorption potentials. The results presented here provide a route to the improvement of detection limits and achievement of selectivity for protein detection by electrochemistry at the ITIES. 2013 Journal Article http://hdl.handle.net/20.500.11937/43997 10.1039/C3AN01123B Royal Society of Chemistry fulltext |
| spellingShingle | adsorptive stripping voltammetry albumin insulin ITIES liquid – liquid interface O'Sullivan, S. De Eulate, E. Yuen, Y. Helmerhorst, Erik Arrigan, Damien Stripping voltammetric detection of insulin at liquid–liquid microinterfaces in the presence of bovine albumin |
| title | Stripping voltammetric detection of insulin at liquid–liquid microinterfaces in the presence of bovine albumin |
| title_full | Stripping voltammetric detection of insulin at liquid–liquid microinterfaces in the presence of bovine albumin |
| title_fullStr | Stripping voltammetric detection of insulin at liquid–liquid microinterfaces in the presence of bovine albumin |
| title_full_unstemmed | Stripping voltammetric detection of insulin at liquid–liquid microinterfaces in the presence of bovine albumin |
| title_short | Stripping voltammetric detection of insulin at liquid–liquid microinterfaces in the presence of bovine albumin |
| title_sort | stripping voltammetric detection of insulin at liquid–liquid microinterfaces in the presence of bovine albumin |
| topic | adsorptive stripping voltammetry albumin insulin ITIES liquid – liquid interface |
| url | http://hdl.handle.net/20.500.11937/43997 |