Identification of Novel Phytase Genes from an Agricultural Soil-Derived Metagenome
Environmental microorganisms are emerging as an important source of new enzymes for wide-scale industrial application. In this study, novel phytase genes were identified from a soil microbial community. For this, a function-based screening approach was utilized for the identification of phytase acti...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Published: |
The Korean Society for Microbiology
2014
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| Online Access: | http://hdl.handle.net/20.500.11937/4398 |
| _version_ | 1848744505366282240 |
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| author | Tan, H. Mooij, M. Barret, M. Hegarty, P. Harrington, C. Dobson, A. O'Gara, Fergal |
| author_facet | Tan, H. Mooij, M. Barret, M. Hegarty, P. Harrington, C. Dobson, A. O'Gara, Fergal |
| author_sort | Tan, H. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Environmental microorganisms are emerging as an important source of new enzymes for wide-scale industrial application. In this study, novel phytase genes were identified from a soil microbial community. For this, a function-based screening approach was utilized for the identification of phytase activity in a metagenomic library derived from an agricultural soil. Two novel phytases were identified. Interestingly, one of these phytases is an unusual histidine acid phosphatase family phytase, as the conserved motif of the active site of PhyX possesses an additional amino acid residue. The second phytase belongs to a new type, which is encoded by multiple open reading frames (ORFs) and is different to all phytases known to date, which are encoded by a single ORF. |
| first_indexed | 2025-11-14T06:02:32Z |
| format | Journal Article |
| id | curtin-20.500.11937-4398 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:02:32Z |
| publishDate | 2014 |
| publisher | The Korean Society for Microbiology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-43982017-09-13T14:43:27Z Identification of Novel Phytase Genes from an Agricultural Soil-Derived Metagenome Tan, H. Mooij, M. Barret, M. Hegarty, P. Harrington, C. Dobson, A. O'Gara, Fergal Environmental microorganisms are emerging as an important source of new enzymes for wide-scale industrial application. In this study, novel phytase genes were identified from a soil microbial community. For this, a function-based screening approach was utilized for the identification of phytase activity in a metagenomic library derived from an agricultural soil. Two novel phytases were identified. Interestingly, one of these phytases is an unusual histidine acid phosphatase family phytase, as the conserved motif of the active site of PhyX possesses an additional amino acid residue. The second phytase belongs to a new type, which is encoded by multiple open reading frames (ORFs) and is different to all phytases known to date, which are encoded by a single ORF. 2014 Journal Article http://hdl.handle.net/20.500.11937/4398 10.4014/jmb.1307.07007 The Korean Society for Microbiology restricted |
| spellingShingle | Tan, H. Mooij, M. Barret, M. Hegarty, P. Harrington, C. Dobson, A. O'Gara, Fergal Identification of Novel Phytase Genes from an Agricultural Soil-Derived Metagenome |
| title | Identification of Novel Phytase Genes from an Agricultural Soil-Derived Metagenome |
| title_full | Identification of Novel Phytase Genes from an Agricultural Soil-Derived Metagenome |
| title_fullStr | Identification of Novel Phytase Genes from an Agricultural Soil-Derived Metagenome |
| title_full_unstemmed | Identification of Novel Phytase Genes from an Agricultural Soil-Derived Metagenome |
| title_short | Identification of Novel Phytase Genes from an Agricultural Soil-Derived Metagenome |
| title_sort | identification of novel phytase genes from an agricultural soil-derived metagenome |
| url | http://hdl.handle.net/20.500.11937/4398 |