Hydrogen isotopic differences between C3 and C4 land plant lipids: consequences of compartmentation in C4 photosynthetic chemistry and C3 photorespiration
The 2H/1H ratio of carbon-bound H in biolipids holds potential for probing plant lipid biosynthesis and metabolism. The biochemical mechanism underlying the isotopic differences between lipids from C3 and C4 plants is still poorly understood. GC-pyrolysis-IRMS (gas chromatography-pyrolysis-isotope r...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
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Wiley
2016
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| Online Access: | http://purl.org/au-research/grants/arc/DP130100577 http://hdl.handle.net/20.500.11937/4373 |
| _version_ | 1848744498346065920 |
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| author | Zhou, Y. Grice, Kliti Stuart-Williams, H. Hocart, C. Gessler, A. Farquhar, G. |
| author_facet | Zhou, Y. Grice, Kliti Stuart-Williams, H. Hocart, C. Gessler, A. Farquhar, G. |
| author_sort | Zhou, Y. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The 2H/1H ratio of carbon-bound H in biolipids holds potential for probing plant lipid biosynthesis and metabolism. The biochemical mechanism underlying the isotopic differences between lipids from C3 and C4 plants is still poorly understood. GC-pyrolysis-IRMS (gas chromatography-pyrolysis-isotope ratio mass spectrometry) measurement of the 2H/1H ratio of leaf lipids from controlled and field grown plants indicates that the biochemical isotopic fractionation (ε2Hlipid_biochem) differed between C3 and C4 plants in a pathway-dependent manner: ε2HC4 > ε2HC3 for the acetogenic pathway, ε2HC4 < ε2HC3 for the mevalonic acid pathway and the 1-deoxy-D-xylulose 5-phosphate pathway across all species examined. It is proposed that compartmentation of photosynthetic CO2 fixation into C4 mesophyll (M) and bundle sheath (BS) cells and suppression of photorespiration in C4 M and BS cells both result in C4 M chloroplastic pyruvate – the precursor for acetogenic pathway – being more depleted in 2H relative to pyruvate in C3 cells. In addition, compartmentation in C4 plants also results in (i) the transferable H of NADPH being enriched in 2H in C4 M chloroplasts compared with that in C3 chloroplasts for the 1-deoxy-D-xylulose 5-phosphate pathway pathway and (ii) pyruvate relatively 2H-enriched being used for the mevalonic acid pathway in the cytosol of BS cells in comparison with that in C3 cells. |
| first_indexed | 2025-11-14T06:02:25Z |
| format | Journal Article |
| id | curtin-20.500.11937-4373 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:02:25Z |
| publishDate | 2016 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-43732022-10-06T04:58:30Z Hydrogen isotopic differences between C3 and C4 land plant lipids: consequences of compartmentation in C4 photosynthetic chemistry and C3 photorespiration Zhou, Y. Grice, Kliti Stuart-Williams, H. Hocart, C. Gessler, A. Farquhar, G. The 2H/1H ratio of carbon-bound H in biolipids holds potential for probing plant lipid biosynthesis and metabolism. The biochemical mechanism underlying the isotopic differences between lipids from C3 and C4 plants is still poorly understood. GC-pyrolysis-IRMS (gas chromatography-pyrolysis-isotope ratio mass spectrometry) measurement of the 2H/1H ratio of leaf lipids from controlled and field grown plants indicates that the biochemical isotopic fractionation (ε2Hlipid_biochem) differed between C3 and C4 plants in a pathway-dependent manner: ε2HC4 > ε2HC3 for the acetogenic pathway, ε2HC4 < ε2HC3 for the mevalonic acid pathway and the 1-deoxy-D-xylulose 5-phosphate pathway across all species examined. It is proposed that compartmentation of photosynthetic CO2 fixation into C4 mesophyll (M) and bundle sheath (BS) cells and suppression of photorespiration in C4 M and BS cells both result in C4 M chloroplastic pyruvate – the precursor for acetogenic pathway – being more depleted in 2H relative to pyruvate in C3 cells. In addition, compartmentation in C4 plants also results in (i) the transferable H of NADPH being enriched in 2H in C4 M chloroplasts compared with that in C3 chloroplasts for the 1-deoxy-D-xylulose 5-phosphate pathway pathway and (ii) pyruvate relatively 2H-enriched being used for the mevalonic acid pathway in the cytosol of BS cells in comparison with that in C3 cells. 2016 Journal Article http://hdl.handle.net/20.500.11937/4373 10.1111/pce.12821 http://purl.org/au-research/grants/arc/DP130100577 Wiley restricted |
| spellingShingle | Zhou, Y. Grice, Kliti Stuart-Williams, H. Hocart, C. Gessler, A. Farquhar, G. Hydrogen isotopic differences between C3 and C4 land plant lipids: consequences of compartmentation in C4 photosynthetic chemistry and C3 photorespiration |
| title | Hydrogen isotopic differences between C3 and C4 land plant lipids: consequences of compartmentation in C4 photosynthetic chemistry and C3 photorespiration |
| title_full | Hydrogen isotopic differences between C3 and C4 land plant lipids: consequences of compartmentation in C4 photosynthetic chemistry and C3 photorespiration |
| title_fullStr | Hydrogen isotopic differences between C3 and C4 land plant lipids: consequences of compartmentation in C4 photosynthetic chemistry and C3 photorespiration |
| title_full_unstemmed | Hydrogen isotopic differences between C3 and C4 land plant lipids: consequences of compartmentation in C4 photosynthetic chemistry and C3 photorespiration |
| title_short | Hydrogen isotopic differences between C3 and C4 land plant lipids: consequences of compartmentation in C4 photosynthetic chemistry and C3 photorespiration |
| title_sort | hydrogen isotopic differences between c3 and c4 land plant lipids: consequences of compartmentation in c4 photosynthetic chemistry and c3 photorespiration |
| url | http://purl.org/au-research/grants/arc/DP130100577 http://hdl.handle.net/20.500.11937/4373 |