Structural features and antitumor activity of a purified polysaccharide extracted from Sargassum horneri
A polysaccharide fraction (SHPSA) was obtained from Sargassum horneri by hot-water extraction and sequential purification of anion-exchange chromatography and gel-filtration chromatography. SHPSA was found to be a neutral polysaccharide fraction with an average molecular weight of 5.78 × 105 Da and...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Published: |
Elsevier
2015
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| Online Access: | http://hdl.handle.net/20.500.11937/43312 |
| _version_ | 1848756656146481152 |
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| author | Shao, P. Liu, J. Chen, X. Fang, Zhongxiang Sun, P. |
| author_facet | Shao, P. Liu, J. Chen, X. Fang, Zhongxiang Sun, P. |
| author_sort | Shao, P. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | A polysaccharide fraction (SHPSA) was obtained from Sargassum horneri by hot-water extraction and sequential purification of anion-exchange chromatography and gel-filtration chromatography. SHPSA was found to be a neutral polysaccharide fraction with an average molecular weight of 5.78 × 105 Da and composed of T-d-Glcp, 1,3-d-Glcp, 1,6-d-Glcp and 1,3,6-d-Glcp in a molar percentage of 1.00:4.17:1.17:0.89, respectively. Based on the results from chemical analysis, NMR, and SHPSA was determined to be a glucan with β-(1 → 6) side chains linked to a β-(1 → 3) backbone with relatively few branch points. Moreover, SHPSA could inhibit the growth of human colon cancer DLD cells in a dose-dependent manner by inducing the apoptosis of DLD cells. So, SHPSA was promising for future use as a natural antitumor agent. |
| first_indexed | 2025-11-14T09:15:40Z |
| format | Journal Article |
| id | curtin-20.500.11937-43312 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:15:40Z |
| publishDate | 2015 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-433122017-09-13T15:08:56Z Structural features and antitumor activity of a purified polysaccharide extracted from Sargassum horneri Shao, P. Liu, J. Chen, X. Fang, Zhongxiang Sun, P. A polysaccharide fraction (SHPSA) was obtained from Sargassum horneri by hot-water extraction and sequential purification of anion-exchange chromatography and gel-filtration chromatography. SHPSA was found to be a neutral polysaccharide fraction with an average molecular weight of 5.78 × 105 Da and composed of T-d-Glcp, 1,3-d-Glcp, 1,6-d-Glcp and 1,3,6-d-Glcp in a molar percentage of 1.00:4.17:1.17:0.89, respectively. Based on the results from chemical analysis, NMR, and SHPSA was determined to be a glucan with β-(1 → 6) side chains linked to a β-(1 → 3) backbone with relatively few branch points. Moreover, SHPSA could inhibit the growth of human colon cancer DLD cells in a dose-dependent manner by inducing the apoptosis of DLD cells. So, SHPSA was promising for future use as a natural antitumor agent. 2015 Journal Article http://hdl.handle.net/20.500.11937/43312 10.1016/j.ijbiomac.2014.10.056 Elsevier restricted |
| spellingShingle | Shao, P. Liu, J. Chen, X. Fang, Zhongxiang Sun, P. Structural features and antitumor activity of a purified polysaccharide extracted from Sargassum horneri |
| title | Structural features and antitumor activity of a purified polysaccharide extracted from Sargassum horneri |
| title_full | Structural features and antitumor activity of a purified polysaccharide extracted from Sargassum horneri |
| title_fullStr | Structural features and antitumor activity of a purified polysaccharide extracted from Sargassum horneri |
| title_full_unstemmed | Structural features and antitumor activity of a purified polysaccharide extracted from Sargassum horneri |
| title_short | Structural features and antitumor activity of a purified polysaccharide extracted from Sargassum horneri |
| title_sort | structural features and antitumor activity of a purified polysaccharide extracted from sargassum horneri |
| url | http://hdl.handle.net/20.500.11937/43312 |