A review on immobilised aptamers for high throughput biomolecular detection and screening
The discovery of Systematic Evolution of Ligands by Exponential Enrichment (SELEX) assay has led to the generation of aptamers from libraries of nucleic acids. Concomitantly, aptamer-target recognition and its potential biomedical applications have become a major research endeavour. Aptamers possess...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Published: |
2015
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| Online Access: | http://hdl.handle.net/20.500.11937/43300 |
| _version_ | 1848756652934692864 |
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| author | Acquah, Caleb Danquah, Michael Yon, John Sidhu, Amandeep Ongkudon, C. |
| author_facet | Acquah, Caleb Danquah, Michael Yon, John Sidhu, Amandeep Ongkudon, C. |
| author_sort | Acquah, Caleb |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The discovery of Systematic Evolution of Ligands by Exponential Enrichment (SELEX) assay has led to the generation of aptamers from libraries of nucleic acids. Concomitantly, aptamer-target recognition and its potential biomedical applications have become a major research endeavour. Aptamers possess unique properties that make them superior biological receptors to antibodies with a plethora of target molecules. Some specific areas of opportunities explored for aptamer-target interactions include biochemical analysis, cell signalling and targeting, biomolecular purification processes, pathogen detection and, clinical diagnosis and therapy. Most of these potential applications rely on the effective immobilisation of aptamers on support systems to probe target species. Hence, recent research focus is geared towards immobilising aptamers as oligosorbents for biodetection and bioscreening. This article seeks to review advances in immobilised aptameric binding with associated successful milestones and respective limitations. A proposal for high throughput bioscreening using continuous polymeric adsorbents is also presented. |
| first_indexed | 2025-11-14T09:15:37Z |
| format | Journal Article |
| id | curtin-20.500.11937-43300 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:15:37Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-433002017-09-13T16:00:09Z A review on immobilised aptamers for high throughput biomolecular detection and screening Acquah, Caleb Danquah, Michael Yon, John Sidhu, Amandeep Ongkudon, C. The discovery of Systematic Evolution of Ligands by Exponential Enrichment (SELEX) assay has led to the generation of aptamers from libraries of nucleic acids. Concomitantly, aptamer-target recognition and its potential biomedical applications have become a major research endeavour. Aptamers possess unique properties that make them superior biological receptors to antibodies with a plethora of target molecules. Some specific areas of opportunities explored for aptamer-target interactions include biochemical analysis, cell signalling and targeting, biomolecular purification processes, pathogen detection and, clinical diagnosis and therapy. Most of these potential applications rely on the effective immobilisation of aptamers on support systems to probe target species. Hence, recent research focus is geared towards immobilising aptamers as oligosorbents for biodetection and bioscreening. This article seeks to review advances in immobilised aptameric binding with associated successful milestones and respective limitations. A proposal for high throughput bioscreening using continuous polymeric adsorbents is also presented. 2015 Journal Article http://hdl.handle.net/20.500.11937/43300 10.1016/j.aca.2015.05.050 fulltext |
| spellingShingle | Acquah, Caleb Danquah, Michael Yon, John Sidhu, Amandeep Ongkudon, C. A review on immobilised aptamers for high throughput biomolecular detection and screening |
| title | A review on immobilised aptamers for high throughput biomolecular detection and screening |
| title_full | A review on immobilised aptamers for high throughput biomolecular detection and screening |
| title_fullStr | A review on immobilised aptamers for high throughput biomolecular detection and screening |
| title_full_unstemmed | A review on immobilised aptamers for high throughput biomolecular detection and screening |
| title_short | A review on immobilised aptamers for high throughput biomolecular detection and screening |
| title_sort | review on immobilised aptamers for high throughput biomolecular detection and screening |
| url | http://hdl.handle.net/20.500.11937/43300 |