Characterisation and cross-species utility of 20 microsatellite markers for population and forensic applications in the endangered Carnaby’s Black-cockatoo, Calyptorhynchus latirostris
We characterise 20 microsatellite loci identifiedfrom the endangered Carnaby’s Black-cockatoo(Calyptorhynchus latirostris). The primers were testedacross 40 individuals from the southwest of WesternAustralia and displayed between 4 and 11 alleles per locuswith expected heterozygosities ranging from...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Published: |
Springer
2009
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| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1007%2Fs12686-009-9079-7 http://hdl.handle.net/20.500.11937/43272 |
| Summary: | We characterise 20 microsatellite loci identifiedfrom the endangered Carnaby’s Black-cockatoo(Calyptorhynchus latirostris). The primers were testedacross 40 individuals from the southwest of WesternAustralia and displayed between 4 and 11 alleles per locuswith expected heterozygosities ranging from 53 to 87% andexclusion probabilities of C0.999. These loci will be usefulin population genetic studies to facilitate conservationmanagement decisions in addition to wildlife enforcementapplications for the endangered Carnaby’s Black-cockatoo.We also tested the markers in 12 high profile and smuggledspecies from five genera, Cacatua, Callocephalon, Calyptorhynchus,Nymphicus and Probosciger. These speciesdetected between 2 and 19 alleles per locus with 50–100%amplification success. |
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