The impact of allylamine-bile acid combinations on cell delivery microcapsules in diabetes
© 2016 Informa UK Limited, trading as Taylor & Francis Group. Objective: In a recent study, we developed a new microencapsulating method for ß-cell microencapsulation, but cell viability declined rapidly, post microencapsulation, due to potential polymer-polyelectrolyte chelation and non-porous...
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| Format: | Journal Article |
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Informa Healthcare
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/43230 |
| _version_ | 1848756633141772288 |
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| author | Mooranian, Armin Negrulj, Rebecca Al-Salami, Hani |
| author_facet | Mooranian, Armin Negrulj, Rebecca Al-Salami, Hani |
| author_sort | Mooranian, Armin |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2016 Informa UK Limited, trading as Taylor & Francis Group. Objective: In a recent study, we developed a new microencapsulating method for ß-cell microencapsulation, but cell viability declined rapidly, post microencapsulation, due to potential polymer-polyelectrolyte chelation and non-porous microcapsules’ membranes resulting in cell apoptosis. Thus, this study tested the effects of incorporating cationic polyamine at 1% w/v, on microcapsule strength and cell viability, in the absence or presence of an anionic tertiary bile acid (ATBA) with potential cell-protective effects. Methods: 1% w/v polyamine was used without or with ATBA, to form ß-cell microcapsules and physical and biological analyses was carried out 50?h post microencapsulation. Results: Microcapsules containing 1% w/v polyamine showed weak physical properties and low cell viability and ATBA incorporation resulted in >30% reduction in cell viability and increased levels of pro-inflammatory cytokines. Conclusion: Neither 1% w/v polyamine nor the presence of ATBA resulted in optimised cell viability, but rather reduced cell viability, enhanced inflammation and lowered insulin secretion. |
| first_indexed | 2025-11-14T09:15:18Z |
| format | Journal Article |
| id | curtin-20.500.11937-43230 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:15:18Z |
| publishDate | 2016 |
| publisher | Informa Healthcare |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-432302017-09-13T15:08:23Z The impact of allylamine-bile acid combinations on cell delivery microcapsules in diabetes Mooranian, Armin Negrulj, Rebecca Al-Salami, Hani © 2016 Informa UK Limited, trading as Taylor & Francis Group. Objective: In a recent study, we developed a new microencapsulating method for ß-cell microencapsulation, but cell viability declined rapidly, post microencapsulation, due to potential polymer-polyelectrolyte chelation and non-porous microcapsules’ membranes resulting in cell apoptosis. Thus, this study tested the effects of incorporating cationic polyamine at 1% w/v, on microcapsule strength and cell viability, in the absence or presence of an anionic tertiary bile acid (ATBA) with potential cell-protective effects. Methods: 1% w/v polyamine was used without or with ATBA, to form ß-cell microcapsules and physical and biological analyses was carried out 50?h post microencapsulation. Results: Microcapsules containing 1% w/v polyamine showed weak physical properties and low cell viability and ATBA incorporation resulted in >30% reduction in cell viability and increased levels of pro-inflammatory cytokines. Conclusion: Neither 1% w/v polyamine nor the presence of ATBA resulted in optimised cell viability, but rather reduced cell viability, enhanced inflammation and lowered insulin secretion. 2016 Journal Article http://hdl.handle.net/20.500.11937/43230 10.1080/02652048.2016.1228703 Informa Healthcare restricted |
| spellingShingle | Mooranian, Armin Negrulj, Rebecca Al-Salami, Hani The impact of allylamine-bile acid combinations on cell delivery microcapsules in diabetes |
| title | The impact of allylamine-bile acid combinations on cell delivery microcapsules in diabetes |
| title_full | The impact of allylamine-bile acid combinations on cell delivery microcapsules in diabetes |
| title_fullStr | The impact of allylamine-bile acid combinations on cell delivery microcapsules in diabetes |
| title_full_unstemmed | The impact of allylamine-bile acid combinations on cell delivery microcapsules in diabetes |
| title_short | The impact of allylamine-bile acid combinations on cell delivery microcapsules in diabetes |
| title_sort | impact of allylamine-bile acid combinations on cell delivery microcapsules in diabetes |
| url | http://hdl.handle.net/20.500.11937/43230 |