AlGaN/GaN-based biosensor for label-free detection of biological activity
In this work, detection of live cell biological activity has been confirmed for label-free AlGaN/GaN based biosensors. Live cells were seeded in solution onto the exposed gate region of packaged AlGaN/GaN field effect transistor (FET) devices and subsequently subjected to various chemical changes in...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Published: |
Elsevier SA
2013
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| Online Access: | http://hdl.handle.net/20.500.11937/43163 |
| _version_ | 1848756614249578496 |
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| author | Podolska, Anna Hool, L Pfleger, K Mishra, U Parish, G Nener, B |
| author_facet | Podolska, Anna Hool, L Pfleger, K Mishra, U Parish, G Nener, B |
| author_sort | Podolska, Anna |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | In this work, detection of live cell biological activity has been confirmed for label-free AlGaN/GaN based biosensors. Live cells were seeded in solution onto the exposed gate region of packaged AlGaN/GaN field effect transistor (FET) devices and subsequently subjected to various chemical changes in the solution. Cell depolarisation with KCl was used to induce biological signals. Significant optimisation of experimental parameters was performed, including environmental conditions and cell seeding concentration. Selectivity towards biological activity was improved by buffering with 30 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES). Reproducible calcium concentration-dependent responses were observed with saturation at the normal physiological level of around 2.5 mM. Cells in a calcium-free solution were pre-treated in separate experiments with calcium ion channel inhibitors Mibefradil, Nisoldipine and HC-030031, and the calcium channel activator S-BayK(-)8644, before calcium dosing. The resulting responses were compared to depolarisation-induced calcium intake and demonstrated physiologically relevant behaviour of calcium ion channels. |
| first_indexed | 2025-11-14T09:15:00Z |
| format | Journal Article |
| id | curtin-20.500.11937-43163 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:15:00Z |
| publishDate | 2013 |
| publisher | Elsevier SA |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-431632017-09-13T15:09:37Z AlGaN/GaN-based biosensor for label-free detection of biological activity Podolska, Anna Hool, L Pfleger, K Mishra, U Parish, G Nener, B In this work, detection of live cell biological activity has been confirmed for label-free AlGaN/GaN based biosensors. Live cells were seeded in solution onto the exposed gate region of packaged AlGaN/GaN field effect transistor (FET) devices and subsequently subjected to various chemical changes in the solution. Cell depolarisation with KCl was used to induce biological signals. Significant optimisation of experimental parameters was performed, including environmental conditions and cell seeding concentration. Selectivity towards biological activity was improved by buffering with 30 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES). Reproducible calcium concentration-dependent responses were observed with saturation at the normal physiological level of around 2.5 mM. Cells in a calcium-free solution were pre-treated in separate experiments with calcium ion channel inhibitors Mibefradil, Nisoldipine and HC-030031, and the calcium channel activator S-BayK(-)8644, before calcium dosing. The resulting responses were compared to depolarisation-induced calcium intake and demonstrated physiologically relevant behaviour of calcium ion channels. 2013 Journal Article http://hdl.handle.net/20.500.11937/43163 10.1016/j.snb.2012.11.086 Elsevier SA restricted |
| spellingShingle | Podolska, Anna Hool, L Pfleger, K Mishra, U Parish, G Nener, B AlGaN/GaN-based biosensor for label-free detection of biological activity |
| title | AlGaN/GaN-based biosensor for label-free detection of biological activity |
| title_full | AlGaN/GaN-based biosensor for label-free detection of biological activity |
| title_fullStr | AlGaN/GaN-based biosensor for label-free detection of biological activity |
| title_full_unstemmed | AlGaN/GaN-based biosensor for label-free detection of biological activity |
| title_short | AlGaN/GaN-based biosensor for label-free detection of biological activity |
| title_sort | algan/gan-based biosensor for label-free detection of biological activity |
| url | http://hdl.handle.net/20.500.11937/43163 |