The effects of long-term saturated fat enriched diets on the brain lipidome
The brain is highly enriched in lipids, where they influence neurotransmission, synaptic plasticity and inflammation. Non-pathological modulation of the brain lipidome has not been previously reported and few studies have investigated the interplay between plasma lipid homeostasis relative to cerebr...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
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Public Library of Science
2016
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| Online Access: | http://purl.org/au-research/grants/nhmrc/1064567 http://hdl.handle.net/20.500.11937/43135 |
| _version_ | 1848756606982946816 |
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| author | Giles, C. Takechi, Ryu Mellett, N. Meikle, P. Dhaliwal, S. Mamo, John |
| author_facet | Giles, C. Takechi, Ryu Mellett, N. Meikle, P. Dhaliwal, S. Mamo, John |
| author_sort | Giles, C. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The brain is highly enriched in lipids, where they influence neurotransmission, synaptic plasticity and inflammation. Non-pathological modulation of the brain lipidome has not been previously reported and few studies have investigated the interplay between plasma lipid homeostasis relative to cerebral lipids. This study explored whether changes in plasma lipids induced by chronic consumption of a well-tolerated diet enriched in saturated fatty acids (SFA) was associated with parallel changes in cerebral lipid homeostasis. Male C57Bl/6 mice were fed regular chow or the SFA diet for six months. Plasma, hippocampus (HPF) and cerebral cortex (CTX) lipids were analysed by LC-ESI-MS/MS. A total of 348 lipid species were determined, comprising 25 lipid classes. The general abundance of HPF and CTX lipids was comparable in SFA fed mice versus controls, despite substantial differences in plasma lipid-class abundance. However, significant differences in 50 specific lipid species were identified as a consequence of SFA treatment, restricted to phosphatidylcholine (PC), phosphatidylethanolamine (PE), alkyl-PC, alkenyl-PC, alkyl-PE, alkenyl-PE, cholesterol ester (CE), diacylglycerol (DG), phosphatidylinositol (PI) and phosphatidylserine (PS) classes. Partial least squares regression of the HPF/CTX lipidome versus plasma lipidome revealed the plasma lipidome could account for a substantial proportion of variation. The findings demonstrate that cerebral abundance of specific lipid species is strongly associated with plasma lipid homeostasis. |
| first_indexed | 2025-11-14T09:14:53Z |
| format | Journal Article |
| id | curtin-20.500.11937-43135 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:14:53Z |
| publishDate | 2016 |
| publisher | Public Library of Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-431352023-04-19T02:26:34Z The effects of long-term saturated fat enriched diets on the brain lipidome Giles, C. Takechi, Ryu Mellett, N. Meikle, P. Dhaliwal, S. Mamo, John The brain is highly enriched in lipids, where they influence neurotransmission, synaptic plasticity and inflammation. Non-pathological modulation of the brain lipidome has not been previously reported and few studies have investigated the interplay between plasma lipid homeostasis relative to cerebral lipids. This study explored whether changes in plasma lipids induced by chronic consumption of a well-tolerated diet enriched in saturated fatty acids (SFA) was associated with parallel changes in cerebral lipid homeostasis. Male C57Bl/6 mice were fed regular chow or the SFA diet for six months. Plasma, hippocampus (HPF) and cerebral cortex (CTX) lipids were analysed by LC-ESI-MS/MS. A total of 348 lipid species were determined, comprising 25 lipid classes. The general abundance of HPF and CTX lipids was comparable in SFA fed mice versus controls, despite substantial differences in plasma lipid-class abundance. However, significant differences in 50 specific lipid species were identified as a consequence of SFA treatment, restricted to phosphatidylcholine (PC), phosphatidylethanolamine (PE), alkyl-PC, alkenyl-PC, alkyl-PE, alkenyl-PE, cholesterol ester (CE), diacylglycerol (DG), phosphatidylinositol (PI) and phosphatidylserine (PS) classes. Partial least squares regression of the HPF/CTX lipidome versus plasma lipidome revealed the plasma lipidome could account for a substantial proportion of variation. The findings demonstrate that cerebral abundance of specific lipid species is strongly associated with plasma lipid homeostasis. 2016 Journal Article http://hdl.handle.net/20.500.11937/43135 10.1371/journal.pone.0166964 http://purl.org/au-research/grants/nhmrc/1064567 Public Library of Science fulltext |
| spellingShingle | Giles, C. Takechi, Ryu Mellett, N. Meikle, P. Dhaliwal, S. Mamo, John The effects of long-term saturated fat enriched diets on the brain lipidome |
| title | The effects of long-term saturated fat enriched diets on the brain lipidome |
| title_full | The effects of long-term saturated fat enriched diets on the brain lipidome |
| title_fullStr | The effects of long-term saturated fat enriched diets on the brain lipidome |
| title_full_unstemmed | The effects of long-term saturated fat enriched diets on the brain lipidome |
| title_short | The effects of long-term saturated fat enriched diets on the brain lipidome |
| title_sort | effects of long-term saturated fat enriched diets on the brain lipidome |
| url | http://purl.org/au-research/grants/nhmrc/1064567 http://hdl.handle.net/20.500.11937/43135 |