Aquimarina amphilecti sp. nov., isolated from the sponge Amphilectus fucorum

A Gram staining negative, rod-shaped orange-coloured, catalase and oxidase positive, non-motile bacterium designated as 92VT was isolated from the marine sponge, Amphilectus fucorum, collected from Lough Hyne, Co. Cork, Ireland. 16S rRNA gene sequence analysis revealed that strain 92VT clustered wit...

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Main Authors: Kennedy, J., Margassery, L., O'Leary, N., O'Gara, Fergal, Morrissey, J., Dobson, A.
Format: Journal Article
Published: Society for General Microbiology 2014
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/42955
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author Kennedy, J.
Margassery, L.
O'Leary, N.
O'Gara, Fergal
Morrissey, J.
Dobson, A.
author_facet Kennedy, J.
Margassery, L.
O'Leary, N.
O'Gara, Fergal
Morrissey, J.
Dobson, A.
author_sort Kennedy, J.
building Curtin Institutional Repository
collection Online Access
description A Gram staining negative, rod-shaped orange-coloured, catalase and oxidase positive, non-motile bacterium designated as 92VT was isolated from the marine sponge, Amphilectus fucorum, collected from Lough Hyne, Co. Cork, Ireland. 16S rRNA gene sequence analysis revealed that strain 92VT clustered with members of the family Flavobacteriaceae, the closest member being Aquimarina latercula NCIMB 1399T, with a gene sequence similarity of 97.5%. Strain 92VT requires seawater for growth with optimal growth occurring at 25°C, pH 6-7 and 3 % (w/v) NaCl. MK-6 is the sole respiratory quinone present and the major fatty acids are iso-C17:0 3-OH, iso-C15:0, iso-C17:1 ?9c and iso-C15:0 3-OH. The DNA G+C content is 36.1 Mol%. Combined phenotypic differences and phylogenetic analysis indicate that strain 92VT represents a novel species of the genus Aquimarina, for which the name Aquimarina amphilecti sp. nov is proposed with strain 92VT as the type strain (=NCIMB 14723T = DSM 25232 T).
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spelling curtin-20.500.11937-429552017-09-13T14:30:53Z Aquimarina amphilecti sp. nov., isolated from the sponge Amphilectus fucorum Kennedy, J. Margassery, L. O'Leary, N. O'Gara, Fergal Morrissey, J. Dobson, A. Marine Aquimarina Sponge A Gram staining negative, rod-shaped orange-coloured, catalase and oxidase positive, non-motile bacterium designated as 92VT was isolated from the marine sponge, Amphilectus fucorum, collected from Lough Hyne, Co. Cork, Ireland. 16S rRNA gene sequence analysis revealed that strain 92VT clustered with members of the family Flavobacteriaceae, the closest member being Aquimarina latercula NCIMB 1399T, with a gene sequence similarity of 97.5%. Strain 92VT requires seawater for growth with optimal growth occurring at 25°C, pH 6-7 and 3 % (w/v) NaCl. MK-6 is the sole respiratory quinone present and the major fatty acids are iso-C17:0 3-OH, iso-C15:0, iso-C17:1 ?9c and iso-C15:0 3-OH. The DNA G+C content is 36.1 Mol%. Combined phenotypic differences and phylogenetic analysis indicate that strain 92VT represents a novel species of the genus Aquimarina, for which the name Aquimarina amphilecti sp. nov is proposed with strain 92VT as the type strain (=NCIMB 14723T = DSM 25232 T). 2014 Journal Article http://hdl.handle.net/20.500.11937/42955 10.1099/ijs.0.049650-0 Society for General Microbiology unknown
spellingShingle Marine
Aquimarina
Sponge
Kennedy, J.
Margassery, L.
O'Leary, N.
O'Gara, Fergal
Morrissey, J.
Dobson, A.
Aquimarina amphilecti sp. nov., isolated from the sponge Amphilectus fucorum
title Aquimarina amphilecti sp. nov., isolated from the sponge Amphilectus fucorum
title_full Aquimarina amphilecti sp. nov., isolated from the sponge Amphilectus fucorum
title_fullStr Aquimarina amphilecti sp. nov., isolated from the sponge Amphilectus fucorum
title_full_unstemmed Aquimarina amphilecti sp. nov., isolated from the sponge Amphilectus fucorum
title_short Aquimarina amphilecti sp. nov., isolated from the sponge Amphilectus fucorum
title_sort aquimarina amphilecti sp. nov., isolated from the sponge amphilectus fucorum
topic Marine
Aquimarina
Sponge
url http://hdl.handle.net/20.500.11937/42955