Breaking the DNA-vaccine bottleneck

Monash University in Australia has developed a new approach towards DNA vaccine development that has the potential to cut the time it takes to produce a vaccine from up to nine months to four weeks or less. The university has designed and filed a patent on a commercially viable, single-stage technol...

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Main Authors: Danquah, Michael, Forde, G.
Format: Journal Article
Published: IChemE 2011
Online Access:http://hdl.handle.net/20.500.11937/42952
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author Danquah, Michael
Forde, G.
author_facet Danquah, Michael
Forde, G.
author_sort Danquah, Michael
building Curtin Institutional Repository
collection Online Access
description Monash University in Australia has developed a new approach towards DNA vaccine development that has the potential to cut the time it takes to produce a vaccine from up to nine months to four weeks or less. The university has designed and filed a patent on a commercially viable, single-stage technology for manufacturing DNA molecules. The technology was used to produce malaria and measles DNA vaccines, which were tested to be homogeneous supercoiled DNA, free from RNA and protein contaminations and meeting FDA regulatory standards for DNA vaccines. The technique is based on customized, smart, polymeric, monolithic adsorbents that can purify DNA very rapidly. The design criteria of solid-phase adsorbent include rapid adsorption and desorption kinetics, physical composition, and adequate selectivity , capacity and recovery. The new show technology significantly improved binding capacities, higher recovery, drastically reduced use of buffers and processing time, less clogging, and higher yields of DNA.
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format Journal Article
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institution Curtin University Malaysia
institution_category Local University
last_indexed 2025-11-14T09:14:06Z
publishDate 2011
publisher IChemE
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spelling curtin-20.500.11937-429522017-01-30T15:03:26Z Breaking the DNA-vaccine bottleneck Danquah, Michael Forde, G. Monash University in Australia has developed a new approach towards DNA vaccine development that has the potential to cut the time it takes to produce a vaccine from up to nine months to four weeks or less. The university has designed and filed a patent on a commercially viable, single-stage technology for manufacturing DNA molecules. The technology was used to produce malaria and measles DNA vaccines, which were tested to be homogeneous supercoiled DNA, free from RNA and protein contaminations and meeting FDA regulatory standards for DNA vaccines. The technique is based on customized, smart, polymeric, monolithic adsorbents that can purify DNA very rapidly. The design criteria of solid-phase adsorbent include rapid adsorption and desorption kinetics, physical composition, and adequate selectivity , capacity and recovery. The new show technology significantly improved binding capacities, higher recovery, drastically reduced use of buffers and processing time, less clogging, and higher yields of DNA. 2011 Journal Article http://hdl.handle.net/20.500.11937/42952 IChemE restricted
spellingShingle Danquah, Michael
Forde, G.
Breaking the DNA-vaccine bottleneck
title Breaking the DNA-vaccine bottleneck
title_full Breaking the DNA-vaccine bottleneck
title_fullStr Breaking the DNA-vaccine bottleneck
title_full_unstemmed Breaking the DNA-vaccine bottleneck
title_short Breaking the DNA-vaccine bottleneck
title_sort breaking the dna-vaccine bottleneck
url http://hdl.handle.net/20.500.11937/42952