Characterization of Leucetta prolifera, a calcarean cyanosponge from south-western Australia, and its symbionts

The biology and ecology of calcarean sponges are not as well understood as they are for demosponges. Here, in order to gain new insights, particularly about symbiotic relationships, the calcarean sponge Leucetta prolifera was sampled from south-western Australia and examined for its assumed photosym...

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Main Authors: Fromont, J., Huggett, M., Lengger, S., Grice, Kliti, Schönberg, C.
Format: Journal Article
Published: Cambridge University Press 2015
Online Access:http://hdl.handle.net/20.500.11937/42780
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author Fromont, J.
Huggett, M.
Lengger, S.
Grice, Kliti
Schönberg, C.
author_facet Fromont, J.
Huggett, M.
Lengger, S.
Grice, Kliti
Schönberg, C.
author_sort Fromont, J.
building Curtin Institutional Repository
collection Online Access
description The biology and ecology of calcarean sponges are not as well understood as they are for demosponges. Here, in order to gain new insights, particularly about symbiotic relationships, the calcarean sponge Leucetta prolifera was sampled from south-western Australia and examined for its assumed photosymbionts. Pulse amplitude modulated fluorometry and extraction of photopigments established that the sponge was photosynthetic. Molecular analysis of the bacterial symbionts via sequencing of the V1–V3 region of the 16S rDNA gene confirmed that between 5 and 22% of all sequences belonged to the phylum Cyanobacteria, depending on the individual sample, with the most dominant strain aligning with Hormoscilla spongeliae, a widely distributed sponge symbiont. Analysis of fatty acids suggested that the sponge obtains nutrition through photosynthates from its symbionts. The relationship is assumed to be mutualistic, with the sponge receiving dietary support and the cyanobacteria sheltering in the sponge tissues. We list all Calcarea presently known to harbour photosymbionts.
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spelling curtin-20.500.11937-427802017-09-13T15:05:24Z Characterization of Leucetta prolifera, a calcarean cyanosponge from south-western Australia, and its symbionts Fromont, J. Huggett, M. Lengger, S. Grice, Kliti Schönberg, C. The biology and ecology of calcarean sponges are not as well understood as they are for demosponges. Here, in order to gain new insights, particularly about symbiotic relationships, the calcarean sponge Leucetta prolifera was sampled from south-western Australia and examined for its assumed photosymbionts. Pulse amplitude modulated fluorometry and extraction of photopigments established that the sponge was photosynthetic. Molecular analysis of the bacterial symbionts via sequencing of the V1–V3 region of the 16S rDNA gene confirmed that between 5 and 22% of all sequences belonged to the phylum Cyanobacteria, depending on the individual sample, with the most dominant strain aligning with Hormoscilla spongeliae, a widely distributed sponge symbiont. Analysis of fatty acids suggested that the sponge obtains nutrition through photosynthates from its symbionts. The relationship is assumed to be mutualistic, with the sponge receiving dietary support and the cyanobacteria sheltering in the sponge tissues. We list all Calcarea presently known to harbour photosymbionts. 2015 Journal Article http://hdl.handle.net/20.500.11937/42780 10.1017/S0025315415000491 Cambridge University Press restricted
spellingShingle Fromont, J.
Huggett, M.
Lengger, S.
Grice, Kliti
Schönberg, C.
Characterization of Leucetta prolifera, a calcarean cyanosponge from south-western Australia, and its symbionts
title Characterization of Leucetta prolifera, a calcarean cyanosponge from south-western Australia, and its symbionts
title_full Characterization of Leucetta prolifera, a calcarean cyanosponge from south-western Australia, and its symbionts
title_fullStr Characterization of Leucetta prolifera, a calcarean cyanosponge from south-western Australia, and its symbionts
title_full_unstemmed Characterization of Leucetta prolifera, a calcarean cyanosponge from south-western Australia, and its symbionts
title_short Characterization of Leucetta prolifera, a calcarean cyanosponge from south-western Australia, and its symbionts
title_sort characterization of leucetta prolifera, a calcarean cyanosponge from south-western australia, and its symbionts
url http://hdl.handle.net/20.500.11937/42780