Identification of Triazine-Resistant Vulpia bromoides

In Australia, triazine herbicides have routinely controlled the Vulpia species (Vulpia bromoides, Vulpia myuros, and Vulpia fasciculata; collectively referred to as silvergrass). However, a simazine-resistant silvergrass biotype, collected from Pingelly in the Western Australian grain belt in 2014,...

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Main Authors: Ashworth, Michael, Han, H., Knell, G., Powles, S.
Format: Journal Article
Published: Weeds Science Society of America 2016
Online Access:http://hdl.handle.net/20.500.11937/42159
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author Ashworth, Michael
Han, H.
Knell, G.
Powles, S.
author_facet Ashworth, Michael
Han, H.
Knell, G.
Powles, S.
author_sort Ashworth, Michael
building Curtin Institutional Repository
collection Online Access
description In Australia, triazine herbicides have routinely controlled the Vulpia species (Vulpia bromoides, Vulpia myuros, and Vulpia fasciculata; collectively referred to as silvergrass). However, a simazine-resistant silvergrass biotype, collected from Pingelly in the Western Australian grain belt in 2014, has been confirmed. Compared to the pooled mortality of three simazine-susceptible silvergrass populations (S1, S2, and S3), the simazine-resistant Pingelly population was > 594-fold resistant at the LD50 level. Dose-response screening of the simazine-selected progeny (> 800 g ai simazine ha−1) demonstrated that the simazine resistance mechanism was heritable. Sequencing of the chloroplast psbA gene revealed the resistant population is homozygous for a serine 264 to glycine mutation, which confers a high-level triazine resistance. As expected this Ser-264-Gly mutation conferred resistance to atrazine and metribuzin, but not the phenyl-urea diuron. This is the first published report confirming field-evolved triazine resistance in a Vulpia population.
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spelling curtin-20.500.11937-421592017-09-13T14:23:34Z Identification of Triazine-Resistant Vulpia bromoides Ashworth, Michael Han, H. Knell, G. Powles, S. In Australia, triazine herbicides have routinely controlled the Vulpia species (Vulpia bromoides, Vulpia myuros, and Vulpia fasciculata; collectively referred to as silvergrass). However, a simazine-resistant silvergrass biotype, collected from Pingelly in the Western Australian grain belt in 2014, has been confirmed. Compared to the pooled mortality of three simazine-susceptible silvergrass populations (S1, S2, and S3), the simazine-resistant Pingelly population was > 594-fold resistant at the LD50 level. Dose-response screening of the simazine-selected progeny (> 800 g ai simazine ha−1) demonstrated that the simazine resistance mechanism was heritable. Sequencing of the chloroplast psbA gene revealed the resistant population is homozygous for a serine 264 to glycine mutation, which confers a high-level triazine resistance. As expected this Ser-264-Gly mutation conferred resistance to atrazine and metribuzin, but not the phenyl-urea diuron. This is the first published report confirming field-evolved triazine resistance in a Vulpia population. 2016 Journal Article http://hdl.handle.net/20.500.11937/42159 10.1614/WT-D-15-00127.1 Weeds Science Society of America restricted
spellingShingle Ashworth, Michael
Han, H.
Knell, G.
Powles, S.
Identification of Triazine-Resistant Vulpia bromoides
title Identification of Triazine-Resistant Vulpia bromoides
title_full Identification of Triazine-Resistant Vulpia bromoides
title_fullStr Identification of Triazine-Resistant Vulpia bromoides
title_full_unstemmed Identification of Triazine-Resistant Vulpia bromoides
title_short Identification of Triazine-Resistant Vulpia bromoides
title_sort identification of triazine-resistant vulpia bromoides
url http://hdl.handle.net/20.500.11937/42159