Optimization of surface plasmon resonance experiments: Case of high mobility group box 1 (HMGB1) interactions
© 2016 Elsevier Inc. All rights reserved. Surface plasmon resonance (SPR) is a powerful technique for evaluating protein-protein interactions in real time. However, inappropriately optimized experiments can often lead to problems in the interpretation of data, leading to unreliable kinetic constants...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Published: |
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/42158 |
| _version_ | 1848756342979821568 |
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| author | Anggayasti, W. Mancera, Ricardo Bottomley, Steven Helmerhorst, Erik |
| author_facet | Anggayasti, W. Mancera, Ricardo Bottomley, Steven Helmerhorst, Erik |
| author_sort | Anggayasti, W. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2016 Elsevier Inc. All rights reserved. Surface plasmon resonance (SPR) is a powerful technique for evaluating protein-protein interactions in real time. However, inappropriately optimized experiments can often lead to problems in the interpretation of data, leading to unreliable kinetic constants and binding models. Optimization of SPR experiments involving "sticky" proteins, or proteins that tend to aggregate, represents a typical scenario where it is important to minimize errors in the data and the kinetic analysis of those data. This is the case of High Mobility Group Box 1 and the receptor of advanced glycation end products. A number of improvements in protein purification, buffer composition, immobilization conditions, and the choice of flow rate are shown to result in substantial improvements in the accurate characterization of the interactions of these proteins and the derivation of the corresponding kinetic constants. |
| first_indexed | 2025-11-14T09:10:41Z |
| format | Journal Article |
| id | curtin-20.500.11937-42158 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:10:41Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-421582017-09-13T14:23:34Z Optimization of surface plasmon resonance experiments: Case of high mobility group box 1 (HMGB1) interactions Anggayasti, W. Mancera, Ricardo Bottomley, Steven Helmerhorst, Erik © 2016 Elsevier Inc. All rights reserved. Surface plasmon resonance (SPR) is a powerful technique for evaluating protein-protein interactions in real time. However, inappropriately optimized experiments can often lead to problems in the interpretation of data, leading to unreliable kinetic constants and binding models. Optimization of SPR experiments involving "sticky" proteins, or proteins that tend to aggregate, represents a typical scenario where it is important to minimize errors in the data and the kinetic analysis of those data. This is the case of High Mobility Group Box 1 and the receptor of advanced glycation end products. A number of improvements in protein purification, buffer composition, immobilization conditions, and the choice of flow rate are shown to result in substantial improvements in the accurate characterization of the interactions of these proteins and the derivation of the corresponding kinetic constants. 2016 Journal Article http://hdl.handle.net/20.500.11937/42158 10.1016/j.ab.2015.12.024 restricted |
| spellingShingle | Anggayasti, W. Mancera, Ricardo Bottomley, Steven Helmerhorst, Erik Optimization of surface plasmon resonance experiments: Case of high mobility group box 1 (HMGB1) interactions |
| title | Optimization of surface plasmon resonance experiments: Case of high mobility group box 1 (HMGB1) interactions |
| title_full | Optimization of surface plasmon resonance experiments: Case of high mobility group box 1 (HMGB1) interactions |
| title_fullStr | Optimization of surface plasmon resonance experiments: Case of high mobility group box 1 (HMGB1) interactions |
| title_full_unstemmed | Optimization of surface plasmon resonance experiments: Case of high mobility group box 1 (HMGB1) interactions |
| title_short | Optimization of surface plasmon resonance experiments: Case of high mobility group box 1 (HMGB1) interactions |
| title_sort | optimization of surface plasmon resonance experiments: case of high mobility group box 1 (hmgb1) interactions |
| url | http://hdl.handle.net/20.500.11937/42158 |