Somatic mutations altering EZH2 (Tyr641) in follicular and diffuse large B-cell lymphomas of germinal-center origin
Follicular lymphoma (FL) and the GCB subtype of diffuse large B-cell lymphoma (DLBCL) derive from germinal center B cells. Targeted resequencing studies have revealed mutations in various genes encoding proteins in the NF-B pathway that contribute to the activated B-cell (ABC) DLBCL subtype, but thu...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
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Nature Publishing Group
2010
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| Online Access: | http://hdl.handle.net/20.500.11937/42144 |
| _version_ | 1848756339194462208 |
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| author | Morin, R. Johnson, N. Severson, T. Mungall, A. An, J. Goya, R. Paul, J. Boyle, M. Woolcock, B. Kuchenbauer, F. Yap, D. Humphries, R. Griffith, O. Shah, S. Zhu, H. Kimbara, M. Shashkin, P. Charlot, J. Tcherpakov, M. Corbett, R. Tam, A. Varhol, Richard Smailus, D. Moksa, M. Zhao, Y. Delaney, A. Qian, H. Birol, I. Schein, J. Moore, R. Holt, R. Horsman, D. Connors, J. Jones, S. Aparicio, S. Hirst, M. Gascoyne, R. Marra, M. |
| author_facet | Morin, R. Johnson, N. Severson, T. Mungall, A. An, J. Goya, R. Paul, J. Boyle, M. Woolcock, B. Kuchenbauer, F. Yap, D. Humphries, R. Griffith, O. Shah, S. Zhu, H. Kimbara, M. Shashkin, P. Charlot, J. Tcherpakov, M. Corbett, R. Tam, A. Varhol, Richard Smailus, D. Moksa, M. Zhao, Y. Delaney, A. Qian, H. Birol, I. Schein, J. Moore, R. Holt, R. Horsman, D. Connors, J. Jones, S. Aparicio, S. Hirst, M. Gascoyne, R. Marra, M. |
| author_sort | Morin, R. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Follicular lymphoma (FL) and the GCB subtype of diffuse large B-cell lymphoma (DLBCL) derive from germinal center B cells. Targeted resequencing studies have revealed mutations in various genes encoding proteins in the NF-B pathway that contribute to the activated B-cell (ABC) DLBCL subtype, but thus far few GCB-specific mutations have been identified. Here we report recurrent somatic mutations affecting the polycomb-group oncogene EZH2, which encodes a histone methyltransferase responsible for trimethylating Lys27 of histone H3 (H3K27). After the recent discovery of mutations in KDM6A (UTX), which encodes the histone H3K27me3 demethylase UTX, in several cancer types, EZH2 is the second histone methyltransferase gene found to be mutated in cancer. These mutations, which result in the replacement of a single tyrosine in the SET domain of the EZH2 protein (Tyr641), occur in 21.7% of GCB DLBCLs and 7.2% of FLs and are absent from ABC DLBCLs. Our data are consistent with the notion that EZH2 proteins with mutant Tyr641 have reduced enzymatic activity in vitro. |
| first_indexed | 2025-11-14T09:10:37Z |
| format | Journal Article |
| id | curtin-20.500.11937-42144 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:10:37Z |
| publishDate | 2010 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-421442018-03-29T09:07:34Z Somatic mutations altering EZH2 (Tyr641) in follicular and diffuse large B-cell lymphomas of germinal-center origin Morin, R. Johnson, N. Severson, T. Mungall, A. An, J. Goya, R. Paul, J. Boyle, M. Woolcock, B. Kuchenbauer, F. Yap, D. Humphries, R. Griffith, O. Shah, S. Zhu, H. Kimbara, M. Shashkin, P. Charlot, J. Tcherpakov, M. Corbett, R. Tam, A. Varhol, Richard Smailus, D. Moksa, M. Zhao, Y. Delaney, A. Qian, H. Birol, I. Schein, J. Moore, R. Holt, R. Horsman, D. Connors, J. Jones, S. Aparicio, S. Hirst, M. Gascoyne, R. Marra, M. Follicular lymphoma (FL) and the GCB subtype of diffuse large B-cell lymphoma (DLBCL) derive from germinal center B cells. Targeted resequencing studies have revealed mutations in various genes encoding proteins in the NF-B pathway that contribute to the activated B-cell (ABC) DLBCL subtype, but thus far few GCB-specific mutations have been identified. Here we report recurrent somatic mutations affecting the polycomb-group oncogene EZH2, which encodes a histone methyltransferase responsible for trimethylating Lys27 of histone H3 (H3K27). After the recent discovery of mutations in KDM6A (UTX), which encodes the histone H3K27me3 demethylase UTX, in several cancer types, EZH2 is the second histone methyltransferase gene found to be mutated in cancer. These mutations, which result in the replacement of a single tyrosine in the SET domain of the EZH2 protein (Tyr641), occur in 21.7% of GCB DLBCLs and 7.2% of FLs and are absent from ABC DLBCLs. Our data are consistent with the notion that EZH2 proteins with mutant Tyr641 have reduced enzymatic activity in vitro. 2010 Journal Article http://hdl.handle.net/20.500.11937/42144 10.1038/ng.518 Nature Publishing Group restricted |
| spellingShingle | Morin, R. Johnson, N. Severson, T. Mungall, A. An, J. Goya, R. Paul, J. Boyle, M. Woolcock, B. Kuchenbauer, F. Yap, D. Humphries, R. Griffith, O. Shah, S. Zhu, H. Kimbara, M. Shashkin, P. Charlot, J. Tcherpakov, M. Corbett, R. Tam, A. Varhol, Richard Smailus, D. Moksa, M. Zhao, Y. Delaney, A. Qian, H. Birol, I. Schein, J. Moore, R. Holt, R. Horsman, D. Connors, J. Jones, S. Aparicio, S. Hirst, M. Gascoyne, R. Marra, M. Somatic mutations altering EZH2 (Tyr641) in follicular and diffuse large B-cell lymphomas of germinal-center origin |
| title | Somatic mutations altering EZH2 (Tyr641) in follicular and diffuse large B-cell lymphomas of germinal-center origin |
| title_full | Somatic mutations altering EZH2 (Tyr641) in follicular and diffuse large B-cell lymphomas of germinal-center origin |
| title_fullStr | Somatic mutations altering EZH2 (Tyr641) in follicular and diffuse large B-cell lymphomas of germinal-center origin |
| title_full_unstemmed | Somatic mutations altering EZH2 (Tyr641) in follicular and diffuse large B-cell lymphomas of germinal-center origin |
| title_short | Somatic mutations altering EZH2 (Tyr641) in follicular and diffuse large B-cell lymphomas of germinal-center origin |
| title_sort | somatic mutations altering ezh2 (tyr641) in follicular and diffuse large b-cell lymphomas of germinal-center origin |
| url | http://hdl.handle.net/20.500.11937/42144 |