Inhibition of destructive autoimmune arthritis in Fc?RIIa transgenic mice by small chemical entities
The interaction of immune complexes with the human Fc receptor, Fc?RIIa, initiates the release of inflammatory mediators and is implicated in the pathogenesis of human autoimmune diseases, including rheumatoid arthritis and systemic lupus erythematosus, so this FcR is a potential target for therapy....
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
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Nature Publishing Group
2009
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| Online Access: | http://hdl.handle.net/20.500.11937/42104 |
| _version_ | 1848756328016642048 |
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| author | Pietersz, G. Mottram, P. Van De Velde, N. Sardjono, C. Esparon, S. Ramsland, Paul Moloney, G. Baell, J. McCarthy, T. Matthews, B. Powell, M. Hogarth, P. |
| author_facet | Pietersz, G. Mottram, P. Van De Velde, N. Sardjono, C. Esparon, S. Ramsland, Paul Moloney, G. Baell, J. McCarthy, T. Matthews, B. Powell, M. Hogarth, P. |
| author_sort | Pietersz, G. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The interaction of immune complexes with the human Fc receptor, Fc?RIIa, initiates the release of inflammatory mediators and is implicated in the pathogenesis of human autoimmune diseases, including rheumatoid arthritis and systemic lupus erythematosus, so this FcR is a potential target for therapy. We have used the three-dimensional structure of an Fc?RIIa dimer to design small molecule inhibitors, modeled on a distinct groove and pocket created by receptor dimerization, adjacent to the ligand-binding sites. These small chemical entities (SCEs) blocked immune complex-induced platelet activation and aggregation and tumor necrosis factor secretion from macrophages in a human cell line and transgenic mouse macrophages. The SCE appeared specific for Fc?RIIa, as they inhibited only immune complex-induced responses and had no effect on responses to stimuli unrelated to FcR, for example platelet stimulation with arachidonic acid. In vivo testing of the SCE in Fc?RIIa transgenic mice showed that they inhibited the development and stopped the progression of collagen-induced arthritis (CIA). The SCEs were more potent than methotrexate and anti-CD3 in sustained suppression of CIA. Thus, in vitro and in vivo activity of these SCE Fc?RIIa receptor antagonists demonstrated their potential as anti-inflammatory agents for autoimmune diseases involving immune complexes. © 2009 Australasian Society for Immunology Inc. All rights reserved. |
| first_indexed | 2025-11-14T09:10:27Z |
| format | Journal Article |
| id | curtin-20.500.11937-42104 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:10:27Z |
| publishDate | 2009 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-421042017-09-13T14:22:51Z Inhibition of destructive autoimmune arthritis in Fc?RIIa transgenic mice by small chemical entities Pietersz, G. Mottram, P. Van De Velde, N. Sardjono, C. Esparon, S. Ramsland, Paul Moloney, G. Baell, J. McCarthy, T. Matthews, B. Powell, M. Hogarth, P. The interaction of immune complexes with the human Fc receptor, Fc?RIIa, initiates the release of inflammatory mediators and is implicated in the pathogenesis of human autoimmune diseases, including rheumatoid arthritis and systemic lupus erythematosus, so this FcR is a potential target for therapy. We have used the three-dimensional structure of an Fc?RIIa dimer to design small molecule inhibitors, modeled on a distinct groove and pocket created by receptor dimerization, adjacent to the ligand-binding sites. These small chemical entities (SCEs) blocked immune complex-induced platelet activation and aggregation and tumor necrosis factor secretion from macrophages in a human cell line and transgenic mouse macrophages. The SCE appeared specific for Fc?RIIa, as they inhibited only immune complex-induced responses and had no effect on responses to stimuli unrelated to FcR, for example platelet stimulation with arachidonic acid. In vivo testing of the SCE in Fc?RIIa transgenic mice showed that they inhibited the development and stopped the progression of collagen-induced arthritis (CIA). The SCEs were more potent than methotrexate and anti-CD3 in sustained suppression of CIA. Thus, in vitro and in vivo activity of these SCE Fc?RIIa receptor antagonists demonstrated their potential as anti-inflammatory agents for autoimmune diseases involving immune complexes. © 2009 Australasian Society for Immunology Inc. All rights reserved. 2009 Journal Article http://hdl.handle.net/20.500.11937/42104 10.1038/icb.2008.82 Nature Publishing Group restricted |
| spellingShingle | Pietersz, G. Mottram, P. Van De Velde, N. Sardjono, C. Esparon, S. Ramsland, Paul Moloney, G. Baell, J. McCarthy, T. Matthews, B. Powell, M. Hogarth, P. Inhibition of destructive autoimmune arthritis in Fc?RIIa transgenic mice by small chemical entities |
| title | Inhibition of destructive autoimmune arthritis in Fc?RIIa transgenic mice by small chemical entities |
| title_full | Inhibition of destructive autoimmune arthritis in Fc?RIIa transgenic mice by small chemical entities |
| title_fullStr | Inhibition of destructive autoimmune arthritis in Fc?RIIa transgenic mice by small chemical entities |
| title_full_unstemmed | Inhibition of destructive autoimmune arthritis in Fc?RIIa transgenic mice by small chemical entities |
| title_short | Inhibition of destructive autoimmune arthritis in Fc?RIIa transgenic mice by small chemical entities |
| title_sort | inhibition of destructive autoimmune arthritis in fc?riia transgenic mice by small chemical entities |
| url | http://hdl.handle.net/20.500.11937/42104 |