Effect of Germination on the Nutritional and Protein Profile of Australian Sweet Lupin (Lupinus angustifolius L.)
Australian Sweet Lupin (ASL) has a nutritional profile ideally suited for human consumption with high protein and fibre, but low starch and fat content. The nutritional and protein profile of germinated ASL may be better than ungerminated ASL and these improvements would provide further benefits in...
| Main Authors: | , , |
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| Format: | Journal Article |
| Published: |
Scientific Research Publishing Inc
2012
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| Online Access: | http://www.scirp.org/journal/PaperInformation.aspx?PaperID=19073 http://hdl.handle.net/20.500.11937/42078 |
| _version_ | 1848756320381960192 |
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| author | Rumiyati, R. James, Anthony Jayasena, Vijay |
| author_facet | Rumiyati, R. James, Anthony Jayasena, Vijay |
| author_sort | Rumiyati, R. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Australian Sweet Lupin (ASL) has a nutritional profile ideally suited for human consumption with high protein and fibre, but low starch and fat content. The nutritional and protein profile of germinated ASL may be better than ungerminated ASL and these improvements would provide further benefits in its use as an ingredient in food applications. In this study the nutritional components such as protein, crude fibre, fat and protein profile of germinated ASL flour following germination at 25°C and 90% - 95% relative humidity for 9 days were determined. The changes in the pattern of ASL protein during germination were analysed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Germination significantly increased crude fibre contents by 450% (db), total protein contents by 38% (db) and decreased the fat content by 70% (db) at day 9 of germination. Electrophoretic analysis of the protein fractions revealed that during germination up to 9 days, some of the high molecular weight proteins disappeared. Germination represents a means to further improve the nutritional profile of the germinated ASL flour with an increased fibre and protein, but lower fat content. |
| first_indexed | 2025-11-14T09:10:19Z |
| format | Journal Article |
| id | curtin-20.500.11937-42078 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:10:19Z |
| publishDate | 2012 |
| publisher | Scientific Research Publishing Inc |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-420782017-03-08T13:11:37Z Effect of Germination on the Nutritional and Protein Profile of Australian Sweet Lupin (Lupinus angustifolius L.) Rumiyati, R. James, Anthony Jayasena, Vijay Australian Sweet Lupin (ASL) has a nutritional profile ideally suited for human consumption with high protein and fibre, but low starch and fat content. The nutritional and protein profile of germinated ASL may be better than ungerminated ASL and these improvements would provide further benefits in its use as an ingredient in food applications. In this study the nutritional components such as protein, crude fibre, fat and protein profile of germinated ASL flour following germination at 25°C and 90% - 95% relative humidity for 9 days were determined. The changes in the pattern of ASL protein during germination were analysed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Germination significantly increased crude fibre contents by 450% (db), total protein contents by 38% (db) and decreased the fat content by 70% (db) at day 9 of germination. Electrophoretic analysis of the protein fractions revealed that during germination up to 9 days, some of the high molecular weight proteins disappeared. Germination represents a means to further improve the nutritional profile of the germinated ASL flour with an increased fibre and protein, but lower fat content. 2012 Journal Article http://hdl.handle.net/20.500.11937/42078 http://www.scirp.org/journal/PaperInformation.aspx?PaperID=19073 Scientific Research Publishing Inc restricted |
| spellingShingle | Rumiyati, R. James, Anthony Jayasena, Vijay Effect of Germination on the Nutritional and Protein Profile of Australian Sweet Lupin (Lupinus angustifolius L.) |
| title | Effect of Germination on the Nutritional and Protein Profile of Australian Sweet Lupin (Lupinus angustifolius L.) |
| title_full | Effect of Germination on the Nutritional and Protein Profile of Australian Sweet Lupin (Lupinus angustifolius L.) |
| title_fullStr | Effect of Germination on the Nutritional and Protein Profile of Australian Sweet Lupin (Lupinus angustifolius L.) |
| title_full_unstemmed | Effect of Germination on the Nutritional and Protein Profile of Australian Sweet Lupin (Lupinus angustifolius L.) |
| title_short | Effect of Germination on the Nutritional and Protein Profile of Australian Sweet Lupin (Lupinus angustifolius L.) |
| title_sort | effect of germination on the nutritional and protein profile of australian sweet lupin (lupinus angustifolius l.) |
| url | http://www.scirp.org/journal/PaperInformation.aspx?PaperID=19073 http://hdl.handle.net/20.500.11937/42078 |