Development of label-free biosensor for the detection of adenosine diphosphate as a universal kinase/atpase assay using nanoimprinted flexible two-dimensional photonic crystal
The use of photonic crystals (PC) as biosensors for label-free detection of kinase activity is presented. Here, we exploited the unique optical properties of PC to detect enzymatic reactions using Protein Kinase A (PKA) as model system. Detection of one of the reaction products, adenosine diphosphat...
| Main Authors: | , , |
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| Format: | Conference Paper |
| Published: |
Chemical and Biological Microsystems Society
2012
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| Online Access: | http://hdl.handle.net/20.500.11937/41465 |
| _version_ | 1848756153459146752 |
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| author | Endo, T. Henares, Bernadette Hisamoto, H. |
| author_facet | Endo, T. Henares, Bernadette Hisamoto, H. |
| author_sort | Endo, T. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The use of photonic crystals (PC) as biosensors for label-free detection of kinase activity is presented. Here, we exploited the unique optical properties of PC to detect enzymatic reactions using Protein Kinase A (PKA) as model system. Detection of one of the reaction products, adenosine diphosphate (ADP) was achieved with sufficient sensitivity using nanoimprinted flexible two-dimensional PC (2D-PC) without the use of tags or dyes. This assay offers a rapid, simple and cost-effective method which can be used as a universal assay procedure to detect kinase reactions. |
| first_indexed | 2025-11-14T09:07:40Z |
| format | Conference Paper |
| id | curtin-20.500.11937-41465 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:07:40Z |
| publishDate | 2012 |
| publisher | Chemical and Biological Microsystems Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-414652017-01-30T14:51:59Z Development of label-free biosensor for the detection of adenosine diphosphate as a universal kinase/atpase assay using nanoimprinted flexible two-dimensional photonic crystal Endo, T. Henares, Bernadette Hisamoto, H. The use of photonic crystals (PC) as biosensors for label-free detection of kinase activity is presented. Here, we exploited the unique optical properties of PC to detect enzymatic reactions using Protein Kinase A (PKA) as model system. Detection of one of the reaction products, adenosine diphosphate (ADP) was achieved with sufficient sensitivity using nanoimprinted flexible two-dimensional PC (2D-PC) without the use of tags or dyes. This assay offers a rapid, simple and cost-effective method which can be used as a universal assay procedure to detect kinase reactions. 2012 Conference Paper http://hdl.handle.net/20.500.11937/41465 Chemical and Biological Microsystems Society restricted |
| spellingShingle | Endo, T. Henares, Bernadette Hisamoto, H. Development of label-free biosensor for the detection of adenosine diphosphate as a universal kinase/atpase assay using nanoimprinted flexible two-dimensional photonic crystal |
| title | Development of label-free biosensor for the detection of adenosine diphosphate as a universal kinase/atpase assay using nanoimprinted flexible two-dimensional photonic crystal |
| title_full | Development of label-free biosensor for the detection of adenosine diphosphate as a universal kinase/atpase assay using nanoimprinted flexible two-dimensional photonic crystal |
| title_fullStr | Development of label-free biosensor for the detection of adenosine diphosphate as a universal kinase/atpase assay using nanoimprinted flexible two-dimensional photonic crystal |
| title_full_unstemmed | Development of label-free biosensor for the detection of adenosine diphosphate as a universal kinase/atpase assay using nanoimprinted flexible two-dimensional photonic crystal |
| title_short | Development of label-free biosensor for the detection of adenosine diphosphate as a universal kinase/atpase assay using nanoimprinted flexible two-dimensional photonic crystal |
| title_sort | development of label-free biosensor for the detection of adenosine diphosphate as a universal kinase/atpase assay using nanoimprinted flexible two-dimensional photonic crystal |
| url | http://hdl.handle.net/20.500.11937/41465 |