Development of label-free biosensor for the detection of adenosine diphosphate as a universal kinase/atpase assay using nanoimprinted flexible two-dimensional photonic crystal
The use of photonic crystals (PC) as biosensors for label-free detection of kinase activity is presented. Here, we exploited the unique optical properties of PC to detect enzymatic reactions using Protein Kinase A (PKA) as model system. Detection of one of the reaction products, adenosine diphosphat...
| Main Authors: | , , |
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| Format: | Conference Paper |
| Published: |
Chemical and Biological Microsystems Society
2012
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| Online Access: | http://hdl.handle.net/20.500.11937/41465 |
| Summary: | The use of photonic crystals (PC) as biosensors for label-free detection of kinase activity is presented. Here, we exploited the unique optical properties of PC to detect enzymatic reactions using Protein Kinase A (PKA) as model system. Detection of one of the reaction products, adenosine diphosphate (ADP) was achieved with sufficient sensitivity using nanoimprinted flexible two-dimensional PC (2D-PC) without the use of tags or dyes. This assay offers a rapid, simple and cost-effective method which can be used as a universal assay procedure to detect kinase reactions. |
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