Genome-wide delineation of natural variation for pod shatter resistance in Brassica napus
Resistance to pod shattering (shatter resistance) is a target trait for global rapeseed (canola, Brassica napus L.), improvement programs to minimise grain loss in the mature standing crop, and during windrowing and mechanical harvest. We describe the genetic basis of natural variation for shatter r...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Journal Article |
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Public Library of Science
2014
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| Online Access: | http://hdl.handle.net/20.500.11937/41295 |
| _version_ | 1848756105052684288 |
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| author | Raman, H. Raman, R. Kilian, A. Detering, F. Carling, J. Coombes, N. Diffey, Simon Kadkol, G. Edwards, D. McCully, M. Ruperao, P. Parkin, I. Batley, J. Luckett, D. Wratten, N. |
| author_facet | Raman, H. Raman, R. Kilian, A. Detering, F. Carling, J. Coombes, N. Diffey, Simon Kadkol, G. Edwards, D. McCully, M. Ruperao, P. Parkin, I. Batley, J. Luckett, D. Wratten, N. |
| author_sort | Raman, H. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Resistance to pod shattering (shatter resistance) is a target trait for global rapeseed (canola, Brassica napus L.), improvement programs to minimise grain loss in the mature standing crop, and during windrowing and mechanical harvest. We describe the genetic basis of natural variation for shatter resistance in B. napus and show that several quantitative trait loci (QTL) control this trait. To identify loci underlying shatter resistance, we used a novel genotyping-by-sequencing approach DArT-Seq. QTL analysis detected a total of 12 significant QTL on chromosomes A03, A07, A09, C03, C04, C06, and C08; which jointly account for approximately 57% of the genotypic variation in shatter resistance. Through Genome-Wide Association Studies, we show that a large number of loci, including those that are involved in shattering in Arabidopsis, account for variation in shatter resistance in diverse B. napus germplasm. Our results indicate that genetic diversity for shatter resistance genes in B. napus is limited; many of the genes that might control this trait were not included during the natural creation of this species, or were not retained during the domestication and selection process. We speculate that valuable diversity for this trait was lost during the natural creation of B. napus. To improve shatter resistance, breeders will need to target the introduction of useful alleles especially from genotypes of other related species of Brassica, such as those that we have identified. © 2014 Raman et al. |
| first_indexed | 2025-11-14T09:06:54Z |
| format | Journal Article |
| id | curtin-20.500.11937-41295 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:06:54Z |
| publishDate | 2014 |
| publisher | Public Library of Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-412952017-09-13T14:09:29Z Genome-wide delineation of natural variation for pod shatter resistance in Brassica napus Raman, H. Raman, R. Kilian, A. Detering, F. Carling, J. Coombes, N. Diffey, Simon Kadkol, G. Edwards, D. McCully, M. Ruperao, P. Parkin, I. Batley, J. Luckett, D. Wratten, N. Resistance to pod shattering (shatter resistance) is a target trait for global rapeseed (canola, Brassica napus L.), improvement programs to minimise grain loss in the mature standing crop, and during windrowing and mechanical harvest. We describe the genetic basis of natural variation for shatter resistance in B. napus and show that several quantitative trait loci (QTL) control this trait. To identify loci underlying shatter resistance, we used a novel genotyping-by-sequencing approach DArT-Seq. QTL analysis detected a total of 12 significant QTL on chromosomes A03, A07, A09, C03, C04, C06, and C08; which jointly account for approximately 57% of the genotypic variation in shatter resistance. Through Genome-Wide Association Studies, we show that a large number of loci, including those that are involved in shattering in Arabidopsis, account for variation in shatter resistance in diverse B. napus germplasm. Our results indicate that genetic diversity for shatter resistance genes in B. napus is limited; many of the genes that might control this trait were not included during the natural creation of this species, or were not retained during the domestication and selection process. We speculate that valuable diversity for this trait was lost during the natural creation of B. napus. To improve shatter resistance, breeders will need to target the introduction of useful alleles especially from genotypes of other related species of Brassica, such as those that we have identified. © 2014 Raman et al. 2014 Journal Article http://hdl.handle.net/20.500.11937/41295 10.1371/journal.pone.0101673 Public Library of Science unknown |
| spellingShingle | Raman, H. Raman, R. Kilian, A. Detering, F. Carling, J. Coombes, N. Diffey, Simon Kadkol, G. Edwards, D. McCully, M. Ruperao, P. Parkin, I. Batley, J. Luckett, D. Wratten, N. Genome-wide delineation of natural variation for pod shatter resistance in Brassica napus |
| title | Genome-wide delineation of natural variation for pod shatter resistance in Brassica napus |
| title_full | Genome-wide delineation of natural variation for pod shatter resistance in Brassica napus |
| title_fullStr | Genome-wide delineation of natural variation for pod shatter resistance in Brassica napus |
| title_full_unstemmed | Genome-wide delineation of natural variation for pod shatter resistance in Brassica napus |
| title_short | Genome-wide delineation of natural variation for pod shatter resistance in Brassica napus |
| title_sort | genome-wide delineation of natural variation for pod shatter resistance in brassica napus |
| url | http://hdl.handle.net/20.500.11937/41295 |