The Molecular Epidemiology of the Highly Virulent ST93 Australian Community Staphylococcus aureus Strain
In Australia the PVL - positive ST93-IV [2B], colloquially known as ‘‘Queensland CA-MRSA’’ has become the dominant CA-MRSA clone. First described in the early 2000s, ST93-IV [2B] is associated with skin and severe invasive infections including necrotizing pneumonia. A singleton by multilocus sequenc...
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| Format: | Journal Article |
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Public Library of Science
2012
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| Online Access: | http://hdl.handle.net/20.500.11937/41002 |
| _version_ | 1848756023550017536 |
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| author | Coombs, Geoffrey Goering, Richard Chua, Kyra Monecke, Stefan Howden, Benjamin Stinear, Timothy Ehricht, Ralf O'Brien, Frances Christiansen, Keryn |
| author_facet | Coombs, Geoffrey Goering, Richard Chua, Kyra Monecke, Stefan Howden, Benjamin Stinear, Timothy Ehricht, Ralf O'Brien, Frances Christiansen, Keryn |
| author_sort | Coombs, Geoffrey |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | In Australia the PVL - positive ST93-IV [2B], colloquially known as ‘‘Queensland CA-MRSA’’ has become the dominant CA-MRSA clone. First described in the early 2000s, ST93-IV [2B] is associated with skin and severe invasive infections including necrotizing pneumonia. A singleton by multilocus sequence typing (MLST) eBURST analysis ST93 is distinct from other S aureus clones. To determine if the increased prevalence of ST93-IV [2B] is due to the widespread transmission of a single strain of ST93-IV [2B] the genetic relatedness of 58 S. aureus ST93 isolated throughout Australia over an extended period were studied in detail using a variety of molecular methods including pulsed-field gel electrophoresis, spa typing, MLST, microarray DNA, SCCmec typing and dru typing. Identification of the phage harbouring the lukS-PV/lukF-PV Panton Valentine leucocidin genes, detection of allelic variations in lukS-PV/lukF-PV, and quantification of LukF-PV expression was also performed. Although ST93-IV [2B] is known to have an apparent enhanced clinical virulence, the isolates harboured few known virulence determinants. All PVL-positive isolates carried the PVL-encoding phage WSa2USA and the lukS-PV/lukF-PV genes had the same R variant SNP profile. The isolates produced similar expression levels of LukF-PV. Although multiple rearrangements of the spa sequence have occurred, the core genome in ST93 is very stable.The emergence of ST93-MRSA is due to independent acquisitions of different dru-defined type IV and type V SCCmec elements in several spa-defined ST93-MSSA backgrounds. Rearrangement of the spa sequence in ST93-MRSA has subsequently occurred in some of these strains. Although multiple ST93-MRSA strains were characterised, little genetic diversity was identified for most isolates, with PVLpositive ST93-IVa [2B]-t202-dt10 predominant across Australia. Whether ST93-IVa [2B] t202-dt10 arose from one PVL-positive ST93-MSSA-t202, or by independent acquisitions of SCCmec-IVa [2B]-dt10 into multiple PVL-positive ST93-MSSA-t202 strains is not known. |
| first_indexed | 2025-11-14T09:05:36Z |
| format | Journal Article |
| id | curtin-20.500.11937-41002 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:05:36Z |
| publishDate | 2012 |
| publisher | Public Library of Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-410022017-09-13T15:58:11Z The Molecular Epidemiology of the Highly Virulent ST93 Australian Community Staphylococcus aureus Strain Coombs, Geoffrey Goering, Richard Chua, Kyra Monecke, Stefan Howden, Benjamin Stinear, Timothy Ehricht, Ralf O'Brien, Frances Christiansen, Keryn ST93-IV [2B] Queensland CA-MRSA CAMRSA skin PVL - positive ST93-IV [2B] In Australia the PVL - positive ST93-IV [2B], colloquially known as ‘‘Queensland CA-MRSA’’ has become the dominant CA-MRSA clone. First described in the early 2000s, ST93-IV [2B] is associated with skin and severe invasive infections including necrotizing pneumonia. A singleton by multilocus sequence typing (MLST) eBURST analysis ST93 is distinct from other S aureus clones. To determine if the increased prevalence of ST93-IV [2B] is due to the widespread transmission of a single strain of ST93-IV [2B] the genetic relatedness of 58 S. aureus ST93 isolated throughout Australia over an extended period were studied in detail using a variety of molecular methods including pulsed-field gel electrophoresis, spa typing, MLST, microarray DNA, SCCmec typing and dru typing. Identification of the phage harbouring the lukS-PV/lukF-PV Panton Valentine leucocidin genes, detection of allelic variations in lukS-PV/lukF-PV, and quantification of LukF-PV expression was also performed. Although ST93-IV [2B] is known to have an apparent enhanced clinical virulence, the isolates harboured few known virulence determinants. All PVL-positive isolates carried the PVL-encoding phage WSa2USA and the lukS-PV/lukF-PV genes had the same R variant SNP profile. The isolates produced similar expression levels of LukF-PV. Although multiple rearrangements of the spa sequence have occurred, the core genome in ST93 is very stable.The emergence of ST93-MRSA is due to independent acquisitions of different dru-defined type IV and type V SCCmec elements in several spa-defined ST93-MSSA backgrounds. Rearrangement of the spa sequence in ST93-MRSA has subsequently occurred in some of these strains. Although multiple ST93-MRSA strains were characterised, little genetic diversity was identified for most isolates, with PVLpositive ST93-IVa [2B]-t202-dt10 predominant across Australia. Whether ST93-IVa [2B] t202-dt10 arose from one PVL-positive ST93-MSSA-t202, or by independent acquisitions of SCCmec-IVa [2B]-dt10 into multiple PVL-positive ST93-MSSA-t202 strains is not known. 2012 Journal Article http://hdl.handle.net/20.500.11937/41002 10.1371/journal.pone.0043037 Public Library of Science fulltext |
| spellingShingle | ST93-IV [2B] Queensland CA-MRSA CAMRSA skin PVL - positive ST93-IV [2B] Coombs, Geoffrey Goering, Richard Chua, Kyra Monecke, Stefan Howden, Benjamin Stinear, Timothy Ehricht, Ralf O'Brien, Frances Christiansen, Keryn The Molecular Epidemiology of the Highly Virulent ST93 Australian Community Staphylococcus aureus Strain |
| title | The Molecular Epidemiology of the Highly Virulent ST93 Australian Community Staphylococcus aureus Strain |
| title_full | The Molecular Epidemiology of the Highly Virulent ST93 Australian Community Staphylococcus aureus Strain |
| title_fullStr | The Molecular Epidemiology of the Highly Virulent ST93 Australian Community Staphylococcus aureus Strain |
| title_full_unstemmed | The Molecular Epidemiology of the Highly Virulent ST93 Australian Community Staphylococcus aureus Strain |
| title_short | The Molecular Epidemiology of the Highly Virulent ST93 Australian Community Staphylococcus aureus Strain |
| title_sort | molecular epidemiology of the highly virulent st93 australian community staphylococcus aureus strain |
| topic | ST93-IV [2B] Queensland CA-MRSA CAMRSA skin PVL - positive ST93-IV [2B] |
| url | http://hdl.handle.net/20.500.11937/41002 |