A rapid non-destructive DNA extraction method for insects and other arthropods
Preparation of arthropods for morphological identification often damages or destroys DNA within the specimen. Conversely, DNA extraction methods often destroy the external physical characteristics essential for morphological identification. We have developed a rapid, simple and non-destructive DNA e...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
Elsevier
2010
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/40898 |
| _version_ | 1848755994358710272 |
|---|---|
| author | Castalanelli, M. Severtson, D. Brumley, C. Szito, A. Foottit, R. Grimm, M. Munyard, Kylie Groth, David |
| author_facet | Castalanelli, M. Severtson, D. Brumley, C. Szito, A. Foottit, R. Grimm, M. Munyard, Kylie Groth, David |
| author_sort | Castalanelli, M. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Preparation of arthropods for morphological identification often damages or destroys DNA within the specimen. Conversely, DNA extraction methods often destroy the external physical characteristics essential for morphological identification. We have developed a rapid, simple and non-destructive DNA extraction technique for arthropod specimens. This technique was tested on four arthropod orders, using specimens that were fresh, preserved by air drying, stored in ethanol, or collected with sticky or propylene glycol traps. The technique could be completed in twenty minutes for Coleoptera, Diptera and Hemiptera, and two minutes for the subclass Acarina, without significant distortion, discolouration, or other damage to the specimens. |
| first_indexed | 2025-11-14T09:05:09Z |
| format | Journal Article |
| id | curtin-20.500.11937-40898 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:05:09Z |
| publishDate | 2010 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-408982017-09-13T16:05:30Z A rapid non-destructive DNA extraction method for insects and other arthropods Castalanelli, M. Severtson, D. Brumley, C. Szito, A. Foottit, R. Grimm, M. Munyard, Kylie Groth, David arthropod Non-destructive DNA taxonomy extraction insect rapid Preparation of arthropods for morphological identification often damages or destroys DNA within the specimen. Conversely, DNA extraction methods often destroy the external physical characteristics essential for morphological identification. We have developed a rapid, simple and non-destructive DNA extraction technique for arthropod specimens. This technique was tested on four arthropod orders, using specimens that were fresh, preserved by air drying, stored in ethanol, or collected with sticky or propylene glycol traps. The technique could be completed in twenty minutes for Coleoptera, Diptera and Hemiptera, and two minutes for the subclass Acarina, without significant distortion, discolouration, or other damage to the specimens. 2010 Journal Article http://hdl.handle.net/20.500.11937/40898 10.1016/j.aspen.2010.04.003 Elsevier fulltext |
| spellingShingle | arthropod Non-destructive DNA taxonomy extraction insect rapid Castalanelli, M. Severtson, D. Brumley, C. Szito, A. Foottit, R. Grimm, M. Munyard, Kylie Groth, David A rapid non-destructive DNA extraction method for insects and other arthropods |
| title | A rapid non-destructive DNA extraction method for insects and other arthropods |
| title_full | A rapid non-destructive DNA extraction method for insects and other arthropods |
| title_fullStr | A rapid non-destructive DNA extraction method for insects and other arthropods |
| title_full_unstemmed | A rapid non-destructive DNA extraction method for insects and other arthropods |
| title_short | A rapid non-destructive DNA extraction method for insects and other arthropods |
| title_sort | rapid non-destructive dna extraction method for insects and other arthropods |
| topic | arthropod Non-destructive DNA taxonomy extraction insect rapid |
| url | http://hdl.handle.net/20.500.11937/40898 |