In vitro propagation of temperate Australian terrestrial orchids: revisiting asymbiotic compared with symbiotic germination
Using a common temperate herbaceous terrestrial orchid from Australia (Caladenia latifolia) this study investigated 19 asymbiotic media variations comprising four commonly used basal media [half-strength Murashige and Skoog (½MS), Knudson C (KC), Pa5, and Vacin and Went (VW), with combinations of th...
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| Format: | Journal Article |
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Wiley-Blackwell
2014
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| Online Access: | http://hdl.handle.net/20.500.11937/40753 |
| _version_ | 1848755955721830400 |
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| author | Bustam, B. Dixon, Kingsley Bunn, E. |
| author_facet | Bustam, B. Dixon, Kingsley Bunn, E. |
| author_sort | Bustam, B. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Using a common temperate herbaceous terrestrial orchid from Australia (Caladenia latifolia) this study investigated 19 asymbiotic media variations comprising four commonly used basal media [half-strength Murashige and Skoog (½MS), Knudson C (KC), Pa5, and Vacin and Went (VW), with combinations of the plant growth regulators 6-benzylaminopurine (BA) and α-naphthalene acetic acid (NAA) or coconut water (CW) and compared their performance with germination on a standard symbiotic germination medium, oatmeal agar (OMA). Percentage germination of seeds every 2 weeks for a total of 8 weeks (five replicates per treatment), time to germination, and growth and development phases in seedlings were recorded. ½MS with 5% (v/v) fresh CW delivered 93% germination, with seedling vigour and development indistinguishable from OMA (95% germination). The same protocol was applied to a further ten species (including the endangered Caladenia huegelii), demonstrating high asymbiotic germination performance (60–93%) across a wide phylogenetic range of terrestrial orchid species. |
| first_indexed | 2025-11-14T09:04:32Z |
| format | Journal Article |
| id | curtin-20.500.11937-40753 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:04:32Z |
| publishDate | 2014 |
| publisher | Wiley-Blackwell |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-407532017-09-13T14:04:51Z In vitro propagation of temperate Australian terrestrial orchids: revisiting asymbiotic compared with symbiotic germination Bustam, B. Dixon, Kingsley Bunn, E. Using a common temperate herbaceous terrestrial orchid from Australia (Caladenia latifolia) this study investigated 19 asymbiotic media variations comprising four commonly used basal media [half-strength Murashige and Skoog (½MS), Knudson C (KC), Pa5, and Vacin and Went (VW), with combinations of the plant growth regulators 6-benzylaminopurine (BA) and α-naphthalene acetic acid (NAA) or coconut water (CW) and compared their performance with germination on a standard symbiotic germination medium, oatmeal agar (OMA). Percentage germination of seeds every 2 weeks for a total of 8 weeks (five replicates per treatment), time to germination, and growth and development phases in seedlings were recorded. ½MS with 5% (v/v) fresh CW delivered 93% germination, with seedling vigour and development indistinguishable from OMA (95% germination). The same protocol was applied to a further ten species (including the endangered Caladenia huegelii), demonstrating high asymbiotic germination performance (60–93%) across a wide phylogenetic range of terrestrial orchid species. 2014 Journal Article http://hdl.handle.net/20.500.11937/40753 10.1111/boj.12216 Wiley-Blackwell unknown |
| spellingShingle | Bustam, B. Dixon, Kingsley Bunn, E. In vitro propagation of temperate Australian terrestrial orchids: revisiting asymbiotic compared with symbiotic germination |
| title | In vitro propagation of temperate Australian terrestrial orchids: revisiting asymbiotic compared with symbiotic germination |
| title_full | In vitro propagation of temperate Australian terrestrial orchids: revisiting asymbiotic compared with symbiotic germination |
| title_fullStr | In vitro propagation of temperate Australian terrestrial orchids: revisiting asymbiotic compared with symbiotic germination |
| title_full_unstemmed | In vitro propagation of temperate Australian terrestrial orchids: revisiting asymbiotic compared with symbiotic germination |
| title_short | In vitro propagation of temperate Australian terrestrial orchids: revisiting asymbiotic compared with symbiotic germination |
| title_sort | in vitro propagation of temperate australian terrestrial orchids: revisiting asymbiotic compared with symbiotic germination |
| url | http://hdl.handle.net/20.500.11937/40753 |