External quality assessment of HLA-B*5701 reporting: An international multicentre survey
Objectives: HLA-B*5701 strongly predicts abacavir hypersensitivity (HSR), but implementation of effective routine screening into clinical practice requires testing be practical and accurate. We tested the proficiency of HLA-B*5701 typing among laboratories using sequence specific primer PCR. Design...
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| Format: | Journal Article |
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International Medical Press
2007
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| Online Access: | http://www.intmedpress.com/serveFile.cfm?sUID=952ab0e5-e84f-4b9a-82ff-999e916f27c5 http://hdl.handle.net/20.500.11937/40639 |
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| author | Hammond, Emma Almeida, Coral-Ann Mamotte, Cyril Nolan, David Phillips, Elizabeth Schollaardt, Tineke Asma Gill, M. John Angel, Jonathan B. Neurath, Doris Li, Jianping Giulivi, Tony McIntyre, Cathy Koultchitski, Galina Wong, Betty Reis, Marciano Rachlis, Anita Cole, David E. Chew, Choo Beng Neifer, Stefan Lalonde, Richard Rogers, Michel Jeanneau, Annie Mallal, Simon |
| author_facet | Hammond, Emma Almeida, Coral-Ann Mamotte, Cyril Nolan, David Phillips, Elizabeth Schollaardt, Tineke Asma Gill, M. John Angel, Jonathan B. Neurath, Doris Li, Jianping Giulivi, Tony McIntyre, Cathy Koultchitski, Galina Wong, Betty Reis, Marciano Rachlis, Anita Cole, David E. Chew, Choo Beng Neifer, Stefan Lalonde, Richard Rogers, Michel Jeanneau, Annie Mallal, Simon |
| author_sort | Hammond, Emma |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Objectives: HLA-B*5701 strongly predicts abacavir hypersensitivity (HSR), but implementation of effective routine screening into clinical practice requires testing be practical and accurate. We tested the proficiency of HLA-B*5701 typing among laboratories using sequence specific primer PCR. Design and methods: DNA panels (1 and 2) were distributed to seven laboratories (A to G) for blinded typing of the HLA-B*5701 allele. Panel 1 (n=10 samples; n=7 laboratories) included 3 positives and other closely related B17 subtypes (B*5702, B*5703, B*5704 and B*5801). Panel 2 (n=96 samples; n=4 laboratories) included 36 positives among a broad spectrum of other B alleles. Two laboratories (A and B) also submitted 96 routine samples, typed by the same methodology, to the reference centre for additional analysis by sequence-based typing. Results: All laboratories correctly typed panel 1 for HLA-B*5701 carriage. Laboratories A, B and C identified HLA-B*5701 alleles in panel 2 with 100% sensitivity and 100% specificity. Laboratory D reported one false negative, reportedly due to a sampling error. The results obtained for routine samples typed by laboratories A and B and those generated by the reference laboratory using sequencing were fully concordant. Conclusions: Detection of HLA-B*5701 alleles among laboratories was 100% specific and 99.4% sensitive, indicating that participating HIV testing laboratories were currently offering effective primary screening to identify individuals at high risk of abacavir HSR. Accurate reporting of HLA-B*5701 status is critical for the safe administration of this drug and participation in quality assurance programmes by all sites who report HLA-B*5701 status should be promoted. |
| first_indexed | 2025-11-14T09:04:03Z |
| format | Journal Article |
| id | curtin-20.500.11937-40639 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:04:03Z |
| publishDate | 2007 |
| publisher | International Medical Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-406392017-01-30T14:44:27Z External quality assessment of HLA-B*5701 reporting: An international multicentre survey Hammond, Emma Almeida, Coral-Ann Mamotte, Cyril Nolan, David Phillips, Elizabeth Schollaardt, Tineke Asma Gill, M. John Angel, Jonathan B. Neurath, Doris Li, Jianping Giulivi, Tony McIntyre, Cathy Koultchitski, Galina Wong, Betty Reis, Marciano Rachlis, Anita Cole, David E. Chew, Choo Beng Neifer, Stefan Lalonde, Richard Rogers, Michel Jeanneau, Annie Mallal, Simon Objectives: HLA-B*5701 strongly predicts abacavir hypersensitivity (HSR), but implementation of effective routine screening into clinical practice requires testing be practical and accurate. We tested the proficiency of HLA-B*5701 typing among laboratories using sequence specific primer PCR. Design and methods: DNA panels (1 and 2) were distributed to seven laboratories (A to G) for blinded typing of the HLA-B*5701 allele. Panel 1 (n=10 samples; n=7 laboratories) included 3 positives and other closely related B17 subtypes (B*5702, B*5703, B*5704 and B*5801). Panel 2 (n=96 samples; n=4 laboratories) included 36 positives among a broad spectrum of other B alleles. Two laboratories (A and B) also submitted 96 routine samples, typed by the same methodology, to the reference centre for additional analysis by sequence-based typing. Results: All laboratories correctly typed panel 1 for HLA-B*5701 carriage. Laboratories A, B and C identified HLA-B*5701 alleles in panel 2 with 100% sensitivity and 100% specificity. Laboratory D reported one false negative, reportedly due to a sampling error. The results obtained for routine samples typed by laboratories A and B and those generated by the reference laboratory using sequencing were fully concordant. Conclusions: Detection of HLA-B*5701 alleles among laboratories was 100% specific and 99.4% sensitive, indicating that participating HIV testing laboratories were currently offering effective primary screening to identify individuals at high risk of abacavir HSR. Accurate reporting of HLA-B*5701 status is critical for the safe administration of this drug and participation in quality assurance programmes by all sites who report HLA-B*5701 status should be promoted. 2007 Journal Article http://hdl.handle.net/20.500.11937/40639 http://www.intmedpress.com/serveFile.cfm?sUID=952ab0e5-e84f-4b9a-82ff-999e916f27c5 International Medical Press restricted |
| spellingShingle | Hammond, Emma Almeida, Coral-Ann Mamotte, Cyril Nolan, David Phillips, Elizabeth Schollaardt, Tineke Asma Gill, M. John Angel, Jonathan B. Neurath, Doris Li, Jianping Giulivi, Tony McIntyre, Cathy Koultchitski, Galina Wong, Betty Reis, Marciano Rachlis, Anita Cole, David E. Chew, Choo Beng Neifer, Stefan Lalonde, Richard Rogers, Michel Jeanneau, Annie Mallal, Simon External quality assessment of HLA-B*5701 reporting: An international multicentre survey |
| title | External quality assessment of HLA-B*5701 reporting: An international multicentre survey |
| title_full | External quality assessment of HLA-B*5701 reporting: An international multicentre survey |
| title_fullStr | External quality assessment of HLA-B*5701 reporting: An international multicentre survey |
| title_full_unstemmed | External quality assessment of HLA-B*5701 reporting: An international multicentre survey |
| title_short | External quality assessment of HLA-B*5701 reporting: An international multicentre survey |
| title_sort | external quality assessment of hla-b*5701 reporting: an international multicentre survey |
| url | http://www.intmedpress.com/serveFile.cfm?sUID=952ab0e5-e84f-4b9a-82ff-999e916f27c5 http://hdl.handle.net/20.500.11937/40639 |