Tumor-infiltrating FOXP3+ T regulatory cells show strong prognostic significance in colorectal cancer

Purpose: To determine the prognostic significance of FOXP3+ lymphocyte (Treg) density in colorectal cancer compared with conventional histopathologic features and with CD8+ and CD45RO+ lymphocyte densities. Patients and Methods: Tissue microarrays and immunohistochemistry were used to assess the den...

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Main Authors: Salama, P., Phillips, M., Grieu, F., Morris, M., Zeps, Nikolajs, Joseph, D., Platell, C., Iacopetta, B.
Format: Journal Article
Published: American Society of Clinical Oncology 2009
Online Access:http://hdl.handle.net/20.500.11937/40045
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author Salama, P.
Phillips, M.
Grieu, F.
Morris, M.
Zeps, Nikolajs
Joseph, D.
Platell, C.
Iacopetta, B.
author_facet Salama, P.
Phillips, M.
Grieu, F.
Morris, M.
Zeps, Nikolajs
Joseph, D.
Platell, C.
Iacopetta, B.
author_sort Salama, P.
building Curtin Institutional Repository
collection Online Access
description Purpose: To determine the prognostic significance of FOXP3+ lymphocyte (Treg) density in colorectal cancer compared with conventional histopathologic features and with CD8+ and CD45RO+ lymphocyte densities. Patients and Methods: Tissue microarrays and immunohistochemistry were used to assess the densities of CD8+, CD45RO+, and FOXP3+ lymphocytes in tumor tissue and normal colonic mucosa from 967 stage II and stage III colorectal cancers. These were evaluated for associations with histopathologic features and patient survival. Results: FOXP3+ Treg density was higher in tumor tissue compared with normal colonic mucosa, whereas CD8+ and CD45RO+ cell densities were lower. FOXP3+ Tregs were not associated with any histopathologic features, with the exception of tumor stage. Multivariate analysis showed that stage, vascular invasion, and FOXP3+ Treg density in normal and tumor tissue were independent prognostic indicators, but not CD8+ and CD45RO+. High FOXP3+ Treg density in normal mucosa was associated with worse prognosis (hazard ratio [HR] = 1.51; 95% CI, 1.07 to 2.13; P = .019). In contrast, a high density of FOXP3 + Tregs in tumor tissue was associated with improved survival (HR = 0.54; 95% CI, 0.38 to 0.77; P = .001). Conclusion: FOXP3+ Treg density in normal and tumor tissue had stronger prognostic significance in colorectal cancer compared with CD8+ and CD45RO+ lymphocytes. The finding of improved survival associated with a high density of tumor-infiltrating FOXP3+ Tregs in colorectal cancer contrasts with several other solid cancer types. The inclusion of FOXP3+ Treg density may help to improve the prognostication of early-stage colorectal cancer. © 2008 by American Society of Clinical Oncology.
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spelling curtin-20.500.11937-400452023-08-02T06:39:08Z Tumor-infiltrating FOXP3+ T regulatory cells show strong prognostic significance in colorectal cancer Salama, P. Phillips, M. Grieu, F. Morris, M. Zeps, Nikolajs Joseph, D. Platell, C. Iacopetta, B. Purpose: To determine the prognostic significance of FOXP3+ lymphocyte (Treg) density in colorectal cancer compared with conventional histopathologic features and with CD8+ and CD45RO+ lymphocyte densities. Patients and Methods: Tissue microarrays and immunohistochemistry were used to assess the densities of CD8+, CD45RO+, and FOXP3+ lymphocytes in tumor tissue and normal colonic mucosa from 967 stage II and stage III colorectal cancers. These were evaluated for associations with histopathologic features and patient survival. Results: FOXP3+ Treg density was higher in tumor tissue compared with normal colonic mucosa, whereas CD8+ and CD45RO+ cell densities were lower. FOXP3+ Tregs were not associated with any histopathologic features, with the exception of tumor stage. Multivariate analysis showed that stage, vascular invasion, and FOXP3+ Treg density in normal and tumor tissue were independent prognostic indicators, but not CD8+ and CD45RO+. High FOXP3+ Treg density in normal mucosa was associated with worse prognosis (hazard ratio [HR] = 1.51; 95% CI, 1.07 to 2.13; P = .019). In contrast, a high density of FOXP3 + Tregs in tumor tissue was associated with improved survival (HR = 0.54; 95% CI, 0.38 to 0.77; P = .001). Conclusion: FOXP3+ Treg density in normal and tumor tissue had stronger prognostic significance in colorectal cancer compared with CD8+ and CD45RO+ lymphocytes. The finding of improved survival associated with a high density of tumor-infiltrating FOXP3+ Tregs in colorectal cancer contrasts with several other solid cancer types. The inclusion of FOXP3+ Treg density may help to improve the prognostication of early-stage colorectal cancer. © 2008 by American Society of Clinical Oncology. 2009 Journal Article http://hdl.handle.net/20.500.11937/40045 10.1200/JCO.2008.18.7229 American Society of Clinical Oncology restricted
spellingShingle Salama, P.
Phillips, M.
Grieu, F.
Morris, M.
Zeps, Nikolajs
Joseph, D.
Platell, C.
Iacopetta, B.
Tumor-infiltrating FOXP3+ T regulatory cells show strong prognostic significance in colorectal cancer
title Tumor-infiltrating FOXP3+ T regulatory cells show strong prognostic significance in colorectal cancer
title_full Tumor-infiltrating FOXP3+ T regulatory cells show strong prognostic significance in colorectal cancer
title_fullStr Tumor-infiltrating FOXP3+ T regulatory cells show strong prognostic significance in colorectal cancer
title_full_unstemmed Tumor-infiltrating FOXP3+ T regulatory cells show strong prognostic significance in colorectal cancer
title_short Tumor-infiltrating FOXP3+ T regulatory cells show strong prognostic significance in colorectal cancer
title_sort tumor-infiltrating foxp3+ t regulatory cells show strong prognostic significance in colorectal cancer
url http://hdl.handle.net/20.500.11937/40045