Functionalized self-assembling peptide improves INS-1 ß-cell function and proliferation via the integrin/FAK/ERK/cyclin pathway
© 2015 Liu et al. Islet transplantation is considered to be a curative treatment for type 1 diabetes mellitus. However, disruption of the extracellular matrix (ECM) leads to ß-cell destruction and graft dysfunction. In this study, we developed a functionalized self-assembling peptide, KLD-F, with EC...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
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2015
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| Online Access: | http://hdl.handle.net/20.500.11937/39904 |
| _version_ | 1848755721177399296 |
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| author | Liu, J. Liu, S. Chen, Younan Zhao, X. Lu, Y. Cheng, J. |
| author_facet | Liu, J. Liu, S. Chen, Younan Zhao, X. Lu, Y. Cheng, J. |
| author_sort | Liu, J. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2015 Liu et al. Islet transplantation is considered to be a curative treatment for type 1 diabetes mellitus. However, disruption of the extracellular matrix (ECM) leads to ß-cell destruction and graft dysfunction. In this study, we developed a functionalized self-assembling peptide, KLD-F, with ECM mimic motifs derived from fibronectin and collagen IV, and evaluated its effect on ß-cell function and proliferation. Atomic force microscopy and rheological results showed that KLD-F could self-assemble into a nanofibrous scaffold and change into a hydrogel in physiological saline condition. In a three-dimensional cell culture model, KLD-F improved ECM remodeling and cell-cell adhesion of INS-1 ß-cells by upregulation of E-cadherin, fibronectin, and collagen IV. KLD-F also enhanced glucose-stimulated insulin secretion and expression of ß-cell function genes, including Glut2, Ins1, MafA, and Pdx-1 in INS-1 cells. Moreover, KLD-F promoted proliferation of INS-1 ß-cells and upregulated Ki67 expression by mediating cell cycle progression. In addition, KLD-F improved ß-cell function and proliferation via an integrin/focal adhesion kinase/extracellular signal-regulated kinase/cyclin D pathway. This study highlights the fact that the ß-cell-ECM interaction reestablished with this functionalized self-assembling peptide is a promising method to improve the therapeutic efficacy of islet transplantation. |
| first_indexed | 2025-11-14T09:00:48Z |
| format | Journal Article |
| id | curtin-20.500.11937-39904 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T09:00:48Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-399042017-09-13T15:08:56Z Functionalized self-assembling peptide improves INS-1 ß-cell function and proliferation via the integrin/FAK/ERK/cyclin pathway Liu, J. Liu, S. Chen, Younan Zhao, X. Lu, Y. Cheng, J. © 2015 Liu et al. Islet transplantation is considered to be a curative treatment for type 1 diabetes mellitus. However, disruption of the extracellular matrix (ECM) leads to ß-cell destruction and graft dysfunction. In this study, we developed a functionalized self-assembling peptide, KLD-F, with ECM mimic motifs derived from fibronectin and collagen IV, and evaluated its effect on ß-cell function and proliferation. Atomic force microscopy and rheological results showed that KLD-F could self-assemble into a nanofibrous scaffold and change into a hydrogel in physiological saline condition. In a three-dimensional cell culture model, KLD-F improved ECM remodeling and cell-cell adhesion of INS-1 ß-cells by upregulation of E-cadherin, fibronectin, and collagen IV. KLD-F also enhanced glucose-stimulated insulin secretion and expression of ß-cell function genes, including Glut2, Ins1, MafA, and Pdx-1 in INS-1 cells. Moreover, KLD-F promoted proliferation of INS-1 ß-cells and upregulated Ki67 expression by mediating cell cycle progression. In addition, KLD-F improved ß-cell function and proliferation via an integrin/focal adhesion kinase/extracellular signal-regulated kinase/cyclin D pathway. This study highlights the fact that the ß-cell-ECM interaction reestablished with this functionalized self-assembling peptide is a promising method to improve the therapeutic efficacy of islet transplantation. 2015 Journal Article http://hdl.handle.net/20.500.11937/39904 10.2147/IJN.S80502 unknown |
| spellingShingle | Liu, J. Liu, S. Chen, Younan Zhao, X. Lu, Y. Cheng, J. Functionalized self-assembling peptide improves INS-1 ß-cell function and proliferation via the integrin/FAK/ERK/cyclin pathway |
| title | Functionalized self-assembling peptide improves INS-1 ß-cell function and proliferation via the integrin/FAK/ERK/cyclin pathway |
| title_full | Functionalized self-assembling peptide improves INS-1 ß-cell function and proliferation via the integrin/FAK/ERK/cyclin pathway |
| title_fullStr | Functionalized self-assembling peptide improves INS-1 ß-cell function and proliferation via the integrin/FAK/ERK/cyclin pathway |
| title_full_unstemmed | Functionalized self-assembling peptide improves INS-1 ß-cell function and proliferation via the integrin/FAK/ERK/cyclin pathway |
| title_short | Functionalized self-assembling peptide improves INS-1 ß-cell function and proliferation via the integrin/FAK/ERK/cyclin pathway |
| title_sort | functionalized self-assembling peptide improves ins-1 ß-cell function and proliferation via the integrin/fak/erk/cyclin pathway |
| url | http://hdl.handle.net/20.500.11937/39904 |