A fluorescence-based quantitative real-time PCR assay for accurate Pocillopora damicornis species identification
Pocillopora damicornis is one of the most extensively studied coral species globally, but high levels of phenotypic plasticity within the genus make species identification based on morphology alone unreliable. As a result, there is a compelling need to develop cheap and time-effective molecular tech...
| Main Authors: | , , , |
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| Format: | Journal Article |
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Springer Verlag
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/38378 |
| _version_ | 1848755304519434240 |
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| author | Thomas, L. Stat, Michael Evans, R. Kennington, W. |
| author_facet | Thomas, L. Stat, Michael Evans, R. Kennington, W. |
| author_sort | Thomas, L. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Pocillopora damicornis is one of the most extensively studied coral species globally, but high levels of phenotypic plasticity within the genus make species identification based on morphology alone unreliable. As a result, there is a compelling need to develop cheap and time-effective molecular techniques capable of accurately distinguishing P. damicornis from other congeneric species. Here, we develop a fluorescence-based quantitative real-time PCR (qPCR) assay to genotype a single nucleotide polymorphism that accurately distinguishes P. damicornis from other morphologically similar Pocillopora species. We trial the assay across colonies representing multiple Pocillopora species and then apply the assay to screen samples of Pocillopora spp. collected at regional scales along the coastline of Western Australia. This assay offers a cheap and time-effective alternative to Sanger sequencing and has broad applications including studies on gene flow, dispersal, recruitment and physiological thresholds of P. damicornis. |
| first_indexed | 2025-11-14T08:54:11Z |
| format | Journal Article |
| id | curtin-20.500.11937-38378 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:54:11Z |
| publishDate | 2016 |
| publisher | Springer Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-383782017-09-13T14:09:30Z A fluorescence-based quantitative real-time PCR assay for accurate Pocillopora damicornis species identification Thomas, L. Stat, Michael Evans, R. Kennington, W. Pocillopora damicornis is one of the most extensively studied coral species globally, but high levels of phenotypic plasticity within the genus make species identification based on morphology alone unreliable. As a result, there is a compelling need to develop cheap and time-effective molecular techniques capable of accurately distinguishing P. damicornis from other congeneric species. Here, we develop a fluorescence-based quantitative real-time PCR (qPCR) assay to genotype a single nucleotide polymorphism that accurately distinguishes P. damicornis from other morphologically similar Pocillopora species. We trial the assay across colonies representing multiple Pocillopora species and then apply the assay to screen samples of Pocillopora spp. collected at regional scales along the coastline of Western Australia. This assay offers a cheap and time-effective alternative to Sanger sequencing and has broad applications including studies on gene flow, dispersal, recruitment and physiological thresholds of P. damicornis. 2016 Journal Article http://hdl.handle.net/20.500.11937/38378 10.1007/s00338-016-1430-3 Springer Verlag restricted |
| spellingShingle | Thomas, L. Stat, Michael Evans, R. Kennington, W. A fluorescence-based quantitative real-time PCR assay for accurate Pocillopora damicornis species identification |
| title | A fluorescence-based quantitative real-time PCR assay for accurate Pocillopora damicornis species identification |
| title_full | A fluorescence-based quantitative real-time PCR assay for accurate Pocillopora damicornis species identification |
| title_fullStr | A fluorescence-based quantitative real-time PCR assay for accurate Pocillopora damicornis species identification |
| title_full_unstemmed | A fluorescence-based quantitative real-time PCR assay for accurate Pocillopora damicornis species identification |
| title_short | A fluorescence-based quantitative real-time PCR assay for accurate Pocillopora damicornis species identification |
| title_sort | fluorescence-based quantitative real-time pcr assay for accurate pocillopora damicornis species identification |
| url | http://hdl.handle.net/20.500.11937/38378 |