An immunological assay for identification of potential biofilm-associated antigens of Staphylococcus aureus
Attachment of bacterial pathogens to the niche tissue in the host is the first step in biofilm formation leading to colonization and establishment of infection in the host. While the most common method used for determining the potential role of a bacterial antigen in biofilm formation has been demon...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Published: |
Springer
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/38024 |
| _version_ | 1848755207395082240 |
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| author | Waryah, Charlene Babra Gogoi-Tiwari, Jully Wells, Kelsi Mukkur, Trilochan |
| author_facet | Waryah, Charlene Babra Gogoi-Tiwari, Jully Wells, Kelsi Mukkur, Trilochan |
| author_sort | Waryah, Charlene Babra |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Attachment of bacterial pathogens to the niche tissue in the host is the first step in biofilm formation leading to colonization and establishment of infection in the host. While the most common method used for determining the potential role of a bacterial antigen in biofilm formation has been demonstration of loss of this property using specific knockout mutants, it is an expensive and a laborious procedure. This study describes an alternative immunological assay for identification of attachment antigens of Staphylococcus aureus, potentially important in the development of an effective vaccine against infections caused by this pathogen. The method is based upon the concept of inhibition of attachment of S. aureus to PEGs coated with virulence antigen-specific antibodies. Antibodies used for validation of this assay were specific for ClfA, FnBPA, SdrD, PNAG and a-toxin, accredited biofilm-associated antigens of S. aureus. |
| first_indexed | 2025-11-14T08:52:38Z |
| format | Journal Article |
| id | curtin-20.500.11937-38024 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:52:38Z |
| publishDate | 2016 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-380242017-09-13T14:09:28Z An immunological assay for identification of potential biofilm-associated antigens of Staphylococcus aureus Waryah, Charlene Babra Gogoi-Tiwari, Jully Wells, Kelsi Mukkur, Trilochan Attachment of bacterial pathogens to the niche tissue in the host is the first step in biofilm formation leading to colonization and establishment of infection in the host. While the most common method used for determining the potential role of a bacterial antigen in biofilm formation has been demonstration of loss of this property using specific knockout mutants, it is an expensive and a laborious procedure. This study describes an alternative immunological assay for identification of attachment antigens of Staphylococcus aureus, potentially important in the development of an effective vaccine against infections caused by this pathogen. The method is based upon the concept of inhibition of attachment of S. aureus to PEGs coated with virulence antigen-specific antibodies. Antibodies used for validation of this assay were specific for ClfA, FnBPA, SdrD, PNAG and a-toxin, accredited biofilm-associated antigens of S. aureus. 2016 Journal Article http://hdl.handle.net/20.500.11937/38024 10.1007/s12223-016-0459-6 Springer restricted |
| spellingShingle | Waryah, Charlene Babra Gogoi-Tiwari, Jully Wells, Kelsi Mukkur, Trilochan An immunological assay for identification of potential biofilm-associated antigens of Staphylococcus aureus |
| title | An immunological assay for identification of potential biofilm-associated antigens of Staphylococcus aureus |
| title_full | An immunological assay for identification of potential biofilm-associated antigens of Staphylococcus aureus |
| title_fullStr | An immunological assay for identification of potential biofilm-associated antigens of Staphylococcus aureus |
| title_full_unstemmed | An immunological assay for identification of potential biofilm-associated antigens of Staphylococcus aureus |
| title_short | An immunological assay for identification of potential biofilm-associated antigens of Staphylococcus aureus |
| title_sort | immunological assay for identification of potential biofilm-associated antigens of staphylococcus aureus |
| url | http://hdl.handle.net/20.500.11937/38024 |