A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite
In this paper, an amperometric biosensor of hydrogen peroxide (H2O2) was fabricated by immobilization of Hemoglobin (Hb) on a Pluronic P123-nanographene platelet (NGP) composite. Direct electron transfer in the Hb-immobilized P123-NGP composite film was greatly facilitated. The surface concentration...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Published: |
Elsevier BV
2011
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| Online Access: | http://hdl.handle.net/20.500.11937/37982 |
| _version_ | 1848755196636692480 |
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| author | Xu, X. Zhang, J. Guo, F. Zheng, W. Zhou, H. Wang, B. Zheng, Y. Wang, Y. Cheng, Y. Lou, Xia Jang, B. |
| author_facet | Xu, X. Zhang, J. Guo, F. Zheng, W. Zhou, H. Wang, B. Zheng, Y. Wang, Y. Cheng, Y. Lou, Xia Jang, B. |
| author_sort | Xu, X. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | In this paper, an amperometric biosensor of hydrogen peroxide (H2O2) was fabricated by immobilization of Hemoglobin (Hb) on a Pluronic P123-nanographene platelet (NGP) composite. Direct electron transfer in the Hb-immobilized P123-NGP composite film was greatly facilitated. The surface concentration (Γ*) and apparent heterogeneous electron transfer rate constant (ks) were calculated to be (1.60 ± 0.17) × 10−10 mol cm−2 and 48.51 s−1, respectively. In addition, the Hb/Pluronic P123-NGP composite showed excellent bioelectrocatalytic activity toward the reduction of H2O2. The biosensor of H2O2 exhibited a linear response to H2O2 in the range of 10–150 μM and a detection limit of 8.24 μM (S/N = 3) was obtained. The apparent Michaelis–Menten constant (K app/m) was 45.35 μM. The resulting biosensor showed fast amperometric response, with very high sensitivity, reliability and effectiveness. |
| first_indexed | 2025-11-14T08:52:28Z |
| format | Journal Article |
| id | curtin-20.500.11937-37982 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:52:28Z |
| publishDate | 2011 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-379822017-09-13T16:08:10Z A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite Xu, X. Zhang, J. Guo, F. Zheng, W. Zhou, H. Wang, B. Zheng, Y. Wang, Y. Cheng, Y. Lou, Xia Jang, B. In this paper, an amperometric biosensor of hydrogen peroxide (H2O2) was fabricated by immobilization of Hemoglobin (Hb) on a Pluronic P123-nanographene platelet (NGP) composite. Direct electron transfer in the Hb-immobilized P123-NGP composite film was greatly facilitated. The surface concentration (Γ*) and apparent heterogeneous electron transfer rate constant (ks) were calculated to be (1.60 ± 0.17) × 10−10 mol cm−2 and 48.51 s−1, respectively. In addition, the Hb/Pluronic P123-NGP composite showed excellent bioelectrocatalytic activity toward the reduction of H2O2. The biosensor of H2O2 exhibited a linear response to H2O2 in the range of 10–150 μM and a detection limit of 8.24 μM (S/N = 3) was obtained. The apparent Michaelis–Menten constant (K app/m) was 45.35 μM. The resulting biosensor showed fast amperometric response, with very high sensitivity, reliability and effectiveness. 2011 Journal Article http://hdl.handle.net/20.500.11937/37982 10.1016/j.colsurfb.2011.01.037 Elsevier BV restricted |
| spellingShingle | Xu, X. Zhang, J. Guo, F. Zheng, W. Zhou, H. Wang, B. Zheng, Y. Wang, Y. Cheng, Y. Lou, Xia Jang, B. A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite |
| title | A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite |
| title_full | A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite |
| title_fullStr | A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite |
| title_full_unstemmed | A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite |
| title_short | A novel amperometric hydrogen peroxide biosensor based on immobilized Hb in Pluronic P123-nanographene platelets composite |
| title_sort | novel amperometric hydrogen peroxide biosensor based on immobilized hb in pluronic p123-nanographene platelets composite |
| url | http://hdl.handle.net/20.500.11937/37982 |