Capturing genetic information using non-target species markers in a species that has undergone a population crash

Species conservation has relied on the enormous potential of information that arises from field, laboratory and other tools. When using molecular-based tools, the technology involves a considerable effort to develop, both in resources and time. A long-held practice has been to utilise pre-existing p...

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Main Authors: Pacioni, Carlo, Spencer, P.
Format: Journal Article
Published: 2010
Online Access:http://hdl.handle.net/20.500.11937/37720
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author Pacioni, Carlo
Spencer, P.
author_facet Pacioni, Carlo
Spencer, P.
author_sort Pacioni, Carlo
building Curtin Institutional Repository
collection Online Access
description Species conservation has relied on the enormous potential of information that arises from field, laboratory and other tools. When using molecular-based tools, the technology involves a considerable effort to develop, both in resources and time. A long-held practice has been to utilise pre-existing primers developed for other closely related species to evaluate conservation questions. In this study, we present a practical approach on how to utilise pre-existing microsatellite markers in bettong and potoroo species. This information is relevant before, during and after a species crash and the approach we describe could be particularly appropriate when there is an immediate need to retrieve a knowledge-base in order to support management decisions. We determined that cross-species amplification success of microsatellite markers is inversely related to evolutionary distance of the source species although their polymorphism is not. A 'priority-list' of potential markers for potoroids is given for future conservation genetic studies. © Australian Mammal Society 2010.
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spelling curtin-20.500.11937-377202018-03-29T09:06:49Z Capturing genetic information using non-target species markers in a species that has undergone a population crash Pacioni, Carlo Spencer, P. Species conservation has relied on the enormous potential of information that arises from field, laboratory and other tools. When using molecular-based tools, the technology involves a considerable effort to develop, both in resources and time. A long-held practice has been to utilise pre-existing primers developed for other closely related species to evaluate conservation questions. In this study, we present a practical approach on how to utilise pre-existing microsatellite markers in bettong and potoroo species. This information is relevant before, during and after a species crash and the approach we describe could be particularly appropriate when there is an immediate need to retrieve a knowledge-base in order to support management decisions. We determined that cross-species amplification success of microsatellite markers is inversely related to evolutionary distance of the source species although their polymorphism is not. A 'priority-list' of potential markers for potoroids is given for future conservation genetic studies. © Australian Mammal Society 2010. 2010 Journal Article http://hdl.handle.net/20.500.11937/37720 10.1071/AM09018 restricted
spellingShingle Pacioni, Carlo
Spencer, P.
Capturing genetic information using non-target species markers in a species that has undergone a population crash
title Capturing genetic information using non-target species markers in a species that has undergone a population crash
title_full Capturing genetic information using non-target species markers in a species that has undergone a population crash
title_fullStr Capturing genetic information using non-target species markers in a species that has undergone a population crash
title_full_unstemmed Capturing genetic information using non-target species markers in a species that has undergone a population crash
title_short Capturing genetic information using non-target species markers in a species that has undergone a population crash
title_sort capturing genetic information using non-target species markers in a species that has undergone a population crash
url http://hdl.handle.net/20.500.11937/37720