Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.)

We report the development of a Diversity Arrays Technology (DArT) marker panel and its utilisation in the development of an integrated genetic linkage map of white lupin (Lupinus albus L.) using an F8 recombinant inbred line population derived from Kiev Mutant/P27174. One hundred and thirty-six DArT...

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Main Authors: Vipin, C., Luckett, D., Harper, J., Ash, G., Kilian, A., Ellwood, Simon, Phan, H., Raman, H.
Format: Journal Article
Published: Nihon Ikushu Gakkai 2013
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/37616
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author Vipin, C.
Luckett, D.
Harper, J.
Ash, G.
Kilian, A.
Ellwood, Simon
Phan, H.
Raman, H.
author_facet Vipin, C.
Luckett, D.
Harper, J.
Ash, G.
Kilian, A.
Ellwood, Simon
Phan, H.
Raman, H.
author_sort Vipin, C.
building Curtin Institutional Repository
collection Online Access
description We report the development of a Diversity Arrays Technology (DArT) marker panel and its utilisation in the development of an integrated genetic linkage map of white lupin (Lupinus albus L.) using an F8 recombinant inbred line population derived from Kiev Mutant/P27174. One hundred and thirty-six DArT markers were merged into the first genetic linkage map composed of 220 amplified fragment length polymorphisms (AFLPs) and 105 genic markers. The integrated map consists of 38 linkage groups of 441 markers and spans a total length of 2,169 cM, with an average interval size of 4.6 cM. The DArT markers exhibited good genome coverage and were associated with previously identified genic and AFLP markers linked with quantitative trait loci for anthracnose resistance, flowering time and alkaloid content. The improved genetic linkage map of white lupin will aid in the identification of markers for traits of interest and future syntenic studies.
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institution Curtin University Malaysia
institution_category Local University
last_indexed 2025-11-14T08:50:54Z
publishDate 2013
publisher Nihon Ikushu Gakkai
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spelling curtin-20.500.11937-376162017-09-13T14:02:53Z Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.) Vipin, C. Luckett, D. Harper, J. Ash, G. Kilian, A. Ellwood, Simon Phan, H. Raman, H. molecular markers Diversity Array Technology Lupinus albus genetic linkage mapping We report the development of a Diversity Arrays Technology (DArT) marker panel and its utilisation in the development of an integrated genetic linkage map of white lupin (Lupinus albus L.) using an F8 recombinant inbred line population derived from Kiev Mutant/P27174. One hundred and thirty-six DArT markers were merged into the first genetic linkage map composed of 220 amplified fragment length polymorphisms (AFLPs) and 105 genic markers. The integrated map consists of 38 linkage groups of 441 markers and spans a total length of 2,169 cM, with an average interval size of 4.6 cM. The DArT markers exhibited good genome coverage and were associated with previously identified genic and AFLP markers linked with quantitative trait loci for anthracnose resistance, flowering time and alkaloid content. The improved genetic linkage map of white lupin will aid in the identification of markers for traits of interest and future syntenic studies. 2013 Journal Article http://hdl.handle.net/20.500.11937/37616 10.1270/jsbbs.63.292 Nihon Ikushu Gakkai fulltext
spellingShingle molecular markers
Diversity Array Technology
Lupinus albus
genetic linkage mapping
Vipin, C.
Luckett, D.
Harper, J.
Ash, G.
Kilian, A.
Ellwood, Simon
Phan, H.
Raman, H.
Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.)
title Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.)
title_full Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.)
title_fullStr Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.)
title_full_unstemmed Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.)
title_short Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.)
title_sort construction of integrated linkage map of a recombinant inbred line population of white lupin (lupinus albus l.)
topic molecular markers
Diversity Array Technology
Lupinus albus
genetic linkage mapping
url http://hdl.handle.net/20.500.11937/37616