Versatile "click chemistry" Approach to functionalizing silicon quantum dots: Applications toward fluorescent cellular imaging
In this study, we describe a solution procedure for the preparation and surface modification of photostable colloidal silicon quantum dots (SiQDs) for imaging of cancer cells. Photoluminescent SiQDs were synthesized by reduction of halogenated silane precursors using a microemulsion process. It was...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
American Chemical Society
2014
|
| Online Access: | http://hdl.handle.net/20.500.11937/37486 |
| _version_ | 1848755061554937856 |
|---|---|
| author | Cheng, X. Lowe, S. Ciampi, Simone Magenau, A. Gaus, K. Reece, P. Gooding, J. |
| author_facet | Cheng, X. Lowe, S. Ciampi, Simone Magenau, A. Gaus, K. Reece, P. Gooding, J. |
| author_sort | Cheng, X. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | In this study, we describe a solution procedure for the preparation and surface modification of photostable colloidal silicon quantum dots (SiQDs) for imaging of cancer cells. Photoluminescent SiQDs were synthesized by reduction of halogenated silane precursors using a microemulsion process. It was shown that 1,8-nonadiyne molecules could be grafted onto the surface of hydrogen-terminated SiQDs via ultraviolet (UV)-promoted hydrosilylation, demonstrated by Fourier transform infrared spectroscopy (FTIR) measurements. In addition, various azide molecules were coupled onto nonadiyne-functionalized particles, rendering particles dispersible in selected polar and nonpolar solvents. The photoluminescence of functionalized SiQDs was stable against photobleaching and did not vary appreciably within biologically applicable pH and temperature ranges. To demonstrate compatibility with biological systems, water-soluble SiQDs were used for fluorescent imaging of HeLa cells. In addition, the SiQDs were shown to be non-cytotoxic at concentrations up to 240 µg/mL. The results presented herein provide good evidence for the versatility of functionalized SiQDs for fluorescent bioimaging application. |
| first_indexed | 2025-11-14T08:50:19Z |
| format | Journal Article |
| id | curtin-20.500.11937-37486 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:50:19Z |
| publishDate | 2014 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-374862017-09-13T13:42:27Z Versatile "click chemistry" Approach to functionalizing silicon quantum dots: Applications toward fluorescent cellular imaging Cheng, X. Lowe, S. Ciampi, Simone Magenau, A. Gaus, K. Reece, P. Gooding, J. In this study, we describe a solution procedure for the preparation and surface modification of photostable colloidal silicon quantum dots (SiQDs) for imaging of cancer cells. Photoluminescent SiQDs were synthesized by reduction of halogenated silane precursors using a microemulsion process. It was shown that 1,8-nonadiyne molecules could be grafted onto the surface of hydrogen-terminated SiQDs via ultraviolet (UV)-promoted hydrosilylation, demonstrated by Fourier transform infrared spectroscopy (FTIR) measurements. In addition, various azide molecules were coupled onto nonadiyne-functionalized particles, rendering particles dispersible in selected polar and nonpolar solvents. The photoluminescence of functionalized SiQDs was stable against photobleaching and did not vary appreciably within biologically applicable pH and temperature ranges. To demonstrate compatibility with biological systems, water-soluble SiQDs were used for fluorescent imaging of HeLa cells. In addition, the SiQDs were shown to be non-cytotoxic at concentrations up to 240 µg/mL. The results presented herein provide good evidence for the versatility of functionalized SiQDs for fluorescent bioimaging application. 2014 Journal Article http://hdl.handle.net/20.500.11937/37486 10.1021/la500945f American Chemical Society restricted |
| spellingShingle | Cheng, X. Lowe, S. Ciampi, Simone Magenau, A. Gaus, K. Reece, P. Gooding, J. Versatile "click chemistry" Approach to functionalizing silicon quantum dots: Applications toward fluorescent cellular imaging |
| title | Versatile "click chemistry" Approach to functionalizing silicon quantum dots: Applications toward fluorescent cellular imaging |
| title_full | Versatile "click chemistry" Approach to functionalizing silicon quantum dots: Applications toward fluorescent cellular imaging |
| title_fullStr | Versatile "click chemistry" Approach to functionalizing silicon quantum dots: Applications toward fluorescent cellular imaging |
| title_full_unstemmed | Versatile "click chemistry" Approach to functionalizing silicon quantum dots: Applications toward fluorescent cellular imaging |
| title_short | Versatile "click chemistry" Approach to functionalizing silicon quantum dots: Applications toward fluorescent cellular imaging |
| title_sort | versatile "click chemistry" approach to functionalizing silicon quantum dots: applications toward fluorescent cellular imaging |
| url | http://hdl.handle.net/20.500.11937/37486 |