Phosphate-selective fluorescent sensing microspheres based on uranyl salophene ionophores

Optical dihydrogen phosphate-selective sensors that function on the basis of bulk optode principles and are based on two different uranyl salophene ionophores are reported here for the first time. The influence of the optode composition and measuring conditions such as sample pH on the optode respon...

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Main Authors: Wygladacz, K., Qin, Y., Wroblewski, W., Bakker, Eric
Format: Journal Article
Published: Elsevier BV 2008
Subjects:
Online Access:http://hdl.handle.net/20.500.11937/37301
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author Wygladacz, K.
Qin, Y.
Wroblewski, W.
Bakker, Eric
author_facet Wygladacz, K.
Qin, Y.
Wroblewski, W.
Bakker, Eric
author_sort Wygladacz, K.
building Curtin Institutional Repository
collection Online Access
description Optical dihydrogen phosphate-selective sensors that function on the basis of bulk optode principles and are based on two different uranyl salophene ionophores are reported here for the first time. The influence of the optode composition and measuring conditions such as sample pH on the optode response are characterized, along with sensor selectivity and long-term stability. Three plasticizers of different polarity are considered for optode fabrication: bis(2-ethylhexyl)sebacate (DOS), dodecyl 2-nitrophenyl ether (o-NPDDE), o-nitrophenyloctylether (o-NPOE). The compounds 9-(diethylamino)-5-(octadecanoylimino)-5H-benzo[a]phenoxazine (ETH 5294, chromoionophore I) and 9-(diethylamino)-5-[(2-octyldecyl)imino]benzo[a]phenoxazine (ETH 5350, chromoionophore III) are used as H+-selective fluoroionophores that also act as reference ionophores. The resulting optode-based sensors are compared with their ion-selective electrode (ISE) counterparts, and it is revealed that optodes are better suited for operation at physiological pH. The best optode performance was found for the two component optode sensors doped with ETH 5350 and phosphate ionophore(I). The linear range of these sensor was log a = −6.0 to −2.6. Dihydrogen phosphate-selective optode sensors of optimized composition are fabricated in microsphere format and preliminary measurements in diluted sheep blood samples are presented.
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spelling curtin-20.500.11937-373012019-02-19T05:35:06Z Phosphate-selective fluorescent sensing microspheres based on uranyl salophene ionophores Wygladacz, K. Qin, Y. Wroblewski, W. Bakker, Eric phosphate sensor ionophore uranyl salophene microsphere sensors optical sensors Optical dihydrogen phosphate-selective sensors that function on the basis of bulk optode principles and are based on two different uranyl salophene ionophores are reported here for the first time. The influence of the optode composition and measuring conditions such as sample pH on the optode response are characterized, along with sensor selectivity and long-term stability. Three plasticizers of different polarity are considered for optode fabrication: bis(2-ethylhexyl)sebacate (DOS), dodecyl 2-nitrophenyl ether (o-NPDDE), o-nitrophenyloctylether (o-NPOE). The compounds 9-(diethylamino)-5-(octadecanoylimino)-5H-benzo[a]phenoxazine (ETH 5294, chromoionophore I) and 9-(diethylamino)-5-[(2-octyldecyl)imino]benzo[a]phenoxazine (ETH 5350, chromoionophore III) are used as H+-selective fluoroionophores that also act as reference ionophores. The resulting optode-based sensors are compared with their ion-selective electrode (ISE) counterparts, and it is revealed that optodes are better suited for operation at physiological pH. The best optode performance was found for the two component optode sensors doped with ETH 5350 and phosphate ionophore(I). The linear range of these sensor was log a = −6.0 to −2.6. Dihydrogen phosphate-selective optode sensors of optimized composition are fabricated in microsphere format and preliminary measurements in diluted sheep blood samples are presented. 2008 Journal Article http://hdl.handle.net/20.500.11937/37301 10.1016/j.aca.2008.02.069 Elsevier BV fulltext
spellingShingle phosphate sensor
ionophore
uranyl salophene
microsphere sensors
optical sensors
Wygladacz, K.
Qin, Y.
Wroblewski, W.
Bakker, Eric
Phosphate-selective fluorescent sensing microspheres based on uranyl salophene ionophores
title Phosphate-selective fluorescent sensing microspheres based on uranyl salophene ionophores
title_full Phosphate-selective fluorescent sensing microspheres based on uranyl salophene ionophores
title_fullStr Phosphate-selective fluorescent sensing microspheres based on uranyl salophene ionophores
title_full_unstemmed Phosphate-selective fluorescent sensing microspheres based on uranyl salophene ionophores
title_short Phosphate-selective fluorescent sensing microspheres based on uranyl salophene ionophores
title_sort phosphate-selective fluorescent sensing microspheres based on uranyl salophene ionophores
topic phosphate sensor
ionophore
uranyl salophene
microsphere sensors
optical sensors
url http://hdl.handle.net/20.500.11937/37301