Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes
Amplified potentiometric transduction of DNA hybridization based on using liposome ‘nanocarriers’ loaded with the signaling ions is reported. The liposome-amplified potentiometric bioassay involved the duplex formation, followed by the capture of calcium-loaded liposomes, a surfactant-induced releas...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Published: |
Royal Society of Chemistry
2010
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| Online Access: | http://hdl.handle.net/20.500.11937/37024 |
| _version_ | 1848754932902002688 |
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| author | Chumbimuni-Torres, K. Wu, J. Clawson, C. Galik, M. Walter, A. Flechsig, G. Bakker, Eric Zhang, L. Wang, J. |
| author_facet | Chumbimuni-Torres, K. Wu, J. Clawson, C. Galik, M. Walter, A. Flechsig, G. Bakker, Eric Zhang, L. Wang, J. |
| author_sort | Chumbimuni-Torres, K. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Amplified potentiometric transduction of DNA hybridization based on using liposome ‘nanocarriers’ loaded with the signaling ions is reported. The liposome-amplified potentiometric bioassay involved the duplex formation, followed by the capture of calcium-loaded liposomes, a surfactant-induced release and highly-sensitive measurements of the calcium signaling ions using a Ca2+ ion-selective electrode (ISE). The high loading yield of nearly one million signaling ions per liposome leads to sub-fmol DNA detection limits. Factors affecting the ion encapsulation efficiency and signal amplification are evaluated and discussed. The influence of the surfactant lysing agent is also examined. Such use of ‘green’ calcium signaling ions addresses the inherent toxicity of Ag and CdS nanoparticle tags used in previous potentiometric bioassays. The new strategy was applied for the detection of low levels of E. coli bacteria. It could be readily extended to trace measurements of other important biomolecules in connection to different biorecognition events. The attractive analytical performance makes liposomes a useful addition to the armory of potentiometric bioassays. |
| first_indexed | 2025-11-14T08:48:16Z |
| format | Journal Article |
| id | curtin-20.500.11937-37024 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:48:16Z |
| publishDate | 2010 |
| publisher | Royal Society of Chemistry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-370242017-09-13T15:52:51Z Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes Chumbimuni-Torres, K. Wu, J. Clawson, C. Galik, M. Walter, A. Flechsig, G. Bakker, Eric Zhang, L. Wang, J. Amplified potentiometric transduction of DNA hybridization based on using liposome ‘nanocarriers’ loaded with the signaling ions is reported. The liposome-amplified potentiometric bioassay involved the duplex formation, followed by the capture of calcium-loaded liposomes, a surfactant-induced release and highly-sensitive measurements of the calcium signaling ions using a Ca2+ ion-selective electrode (ISE). The high loading yield of nearly one million signaling ions per liposome leads to sub-fmol DNA detection limits. Factors affecting the ion encapsulation efficiency and signal amplification are evaluated and discussed. The influence of the surfactant lysing agent is also examined. Such use of ‘green’ calcium signaling ions addresses the inherent toxicity of Ag and CdS nanoparticle tags used in previous potentiometric bioassays. The new strategy was applied for the detection of low levels of E. coli bacteria. It could be readily extended to trace measurements of other important biomolecules in connection to different biorecognition events. The attractive analytical performance makes liposomes a useful addition to the armory of potentiometric bioassays. 2010 Journal Article http://hdl.handle.net/20.500.11937/37024 10.1039/c0an00198h Royal Society of Chemistry restricted |
| spellingShingle | Chumbimuni-Torres, K. Wu, J. Clawson, C. Galik, M. Walter, A. Flechsig, G. Bakker, Eric Zhang, L. Wang, J. Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes |
| title | Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes |
| title_full | Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes |
| title_fullStr | Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes |
| title_full_unstemmed | Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes |
| title_short | Amplified potentiometric transduction of DNA hybridization using ion-loaded liposomes |
| title_sort | amplified potentiometric transduction of dna hybridization using ion-loaded liposomes |
| url | http://hdl.handle.net/20.500.11937/37024 |