Structural basis for Fc?RIIa recognition of human IgG and formation of inflammatory signaling complexes
The interaction of Abs with their specific FcRs is of primary importance in host immune effector systems involved in infection and inflammation, and are the target for immune evasion by pathogens. Fc?RIIa is a unique and the most widespread activating FcR in humans that through avid binding of immun...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Published: |
2011
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| Online Access: | http://hdl.handle.net/20.500.11937/3683 |
| _version_ | 1848744298654203904 |
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| author | Ramsland, Paul Farrugia, W. Bradford, T. Sardjono, C. Esparon, S. Trist, H. Powell, M. Tan, P. Cendron, A. Wines, B. Scott, A. Hogarth, P. |
| author_facet | Ramsland, Paul Farrugia, W. Bradford, T. Sardjono, C. Esparon, S. Trist, H. Powell, M. Tan, P. Cendron, A. Wines, B. Scott, A. Hogarth, P. |
| author_sort | Ramsland, Paul |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The interaction of Abs with their specific FcRs is of primary importance in host immune effector systems involved in infection and inflammation, and are the target for immune evasion by pathogens. Fc?RIIa is a unique and the most widespread activating FcR in humans that through avid binding of immune complexes potently triggers inflammation. Polymorphisms of Fc?RIIa (high responder/low responder [HR/LR]) are linked to susceptibility to infections, autoimmune diseases, and the efficacy of therapeutic Abs. In this article, we define the three-dimensional structure of the complex between the HR (arginine, R134) allele of Fc?RIIa (Fc?RIIa-HR) and the Fc region of a humanized IgG1 Ab, hu3S193. The structure suggests how the HR/LR polymorphism may influence Fc?RIIa interactions with different IgG subclasses and glycoforms. In addition, mutagenesis defined the basis of the epitopes detected by FcR blocking mAbs specific for Fc?RIIa (IV.3), Fc?RIIb (X63-21), and a pan Fc?RII Ab (8.7). The epitopes detected by these Abs are distinct, but all overlap with residues defined by crystallography to contact IgG. Finally, crystal structures of LR (histidine, H134) allele of Fc?RIIa and Fc?RIIa-HR reveal two distinct receptor dimers that may represent quaternary states on the cell surface. A model is presented whereby a dimer of Fc?RIIa-HR binds Ag-Ab complexes in an arrangement that possibly occurs on the cell membrane as part of a larger signaling assembly. Copyright © 2011 by The American Association of Immunologists, Inc. |
| first_indexed | 2025-11-14T05:59:15Z |
| format | Journal Article |
| id | curtin-20.500.11937-3683 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T05:59:15Z |
| publishDate | 2011 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-36832017-09-13T14:44:01Z Structural basis for Fc?RIIa recognition of human IgG and formation of inflammatory signaling complexes Ramsland, Paul Farrugia, W. Bradford, T. Sardjono, C. Esparon, S. Trist, H. Powell, M. Tan, P. Cendron, A. Wines, B. Scott, A. Hogarth, P. The interaction of Abs with their specific FcRs is of primary importance in host immune effector systems involved in infection and inflammation, and are the target for immune evasion by pathogens. Fc?RIIa is a unique and the most widespread activating FcR in humans that through avid binding of immune complexes potently triggers inflammation. Polymorphisms of Fc?RIIa (high responder/low responder [HR/LR]) are linked to susceptibility to infections, autoimmune diseases, and the efficacy of therapeutic Abs. In this article, we define the three-dimensional structure of the complex between the HR (arginine, R134) allele of Fc?RIIa (Fc?RIIa-HR) and the Fc region of a humanized IgG1 Ab, hu3S193. The structure suggests how the HR/LR polymorphism may influence Fc?RIIa interactions with different IgG subclasses and glycoforms. In addition, mutagenesis defined the basis of the epitopes detected by FcR blocking mAbs specific for Fc?RIIa (IV.3), Fc?RIIb (X63-21), and a pan Fc?RII Ab (8.7). The epitopes detected by these Abs are distinct, but all overlap with residues defined by crystallography to contact IgG. Finally, crystal structures of LR (histidine, H134) allele of Fc?RIIa and Fc?RIIa-HR reveal two distinct receptor dimers that may represent quaternary states on the cell surface. A model is presented whereby a dimer of Fc?RIIa-HR binds Ag-Ab complexes in an arrangement that possibly occurs on the cell membrane as part of a larger signaling assembly. Copyright © 2011 by The American Association of Immunologists, Inc. 2011 Journal Article http://hdl.handle.net/20.500.11937/3683 10.4049/jimmunol.1101467 unknown |
| spellingShingle | Ramsland, Paul Farrugia, W. Bradford, T. Sardjono, C. Esparon, S. Trist, H. Powell, M. Tan, P. Cendron, A. Wines, B. Scott, A. Hogarth, P. Structural basis for Fc?RIIa recognition of human IgG and formation of inflammatory signaling complexes |
| title | Structural basis for Fc?RIIa recognition of human IgG and formation of inflammatory signaling complexes |
| title_full | Structural basis for Fc?RIIa recognition of human IgG and formation of inflammatory signaling complexes |
| title_fullStr | Structural basis for Fc?RIIa recognition of human IgG and formation of inflammatory signaling complexes |
| title_full_unstemmed | Structural basis for Fc?RIIa recognition of human IgG and formation of inflammatory signaling complexes |
| title_short | Structural basis for Fc?RIIa recognition of human IgG and formation of inflammatory signaling complexes |
| title_sort | structural basis for fc?riia recognition of human igg and formation of inflammatory signaling complexes |
| url | http://hdl.handle.net/20.500.11937/3683 |