Molecular genetic and physical analysis of gas vesicles in buoyant enterobacteria
© 2016 Society for Applied Microbiology and John Wiley & Sons Ltd. Different modes of bacterial taxis play important roles in environmental adaptation, survival, colonization and dissemination of disease. One mode of taxis is flotation due to the production of gas vesicles. Gas vesicles are prot...
| Main Authors: | , , , |
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| Format: | Journal Article |
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Wiley-Blackwell Publishing
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/36437 |
| _version_ | 1848754770344411136 |
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| author | Tashiro, Y. Monson, R. Ramsay, Joshua Salmond, G. |
| author_facet | Tashiro, Y. Monson, R. Ramsay, Joshua Salmond, G. |
| author_sort | Tashiro, Y. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2016 Society for Applied Microbiology and John Wiley & Sons Ltd. Different modes of bacterial taxis play important roles in environmental adaptation, survival, colonization and dissemination of disease. One mode of taxis is flotation due to the production of gas vesicles. Gas vesicles are proteinaceous intracellular organelles, permeable only to gas, that enable flotation in aquatic niches. Gene clusters for gas vesicle biosynthesis are partially conserved in various archaea, cyanobacteria, and some proteobacteria, such as the enterobacterium, Serratia sp. ATCC 39006 (S39006). Here we present the first systematic analysis of the genes required to produce gas vesicles in S39006, identifying how this differs from the archaeon Halobacterium salinarum. We define 11 proteins essential for gas vesicle production. Mutation of gvpN or gvpV produced small bicone gas vesicles, suggesting that the cognate proteins are involved in the morphogenetic assembly pathway from bicones to mature cylindrical forms. Using volumetric compression, gas vesicles were shown to comprise 17% of S39006 cells, whereas in Escherichia coli heterologously expressing the gas vesicle cluster in a deregulated environment, gas vesicles can occupy around half of cellular volume. Gas vesicle production in S39006 and E.coli was exploited to calculate the instantaneous turgor pressure within cultured bacterial cells; the first time this has been performed in either strain. |
| first_indexed | 2025-11-14T08:45:41Z |
| format | Journal Article |
| id | curtin-20.500.11937-36437 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:45:41Z |
| publishDate | 2016 |
| publisher | Wiley-Blackwell Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-364372017-09-13T15:24:21Z Molecular genetic and physical analysis of gas vesicles in buoyant enterobacteria Tashiro, Y. Monson, R. Ramsay, Joshua Salmond, G. © 2016 Society for Applied Microbiology and John Wiley & Sons Ltd. Different modes of bacterial taxis play important roles in environmental adaptation, survival, colonization and dissemination of disease. One mode of taxis is flotation due to the production of gas vesicles. Gas vesicles are proteinaceous intracellular organelles, permeable only to gas, that enable flotation in aquatic niches. Gene clusters for gas vesicle biosynthesis are partially conserved in various archaea, cyanobacteria, and some proteobacteria, such as the enterobacterium, Serratia sp. ATCC 39006 (S39006). Here we present the first systematic analysis of the genes required to produce gas vesicles in S39006, identifying how this differs from the archaeon Halobacterium salinarum. We define 11 proteins essential for gas vesicle production. Mutation of gvpN or gvpV produced small bicone gas vesicles, suggesting that the cognate proteins are involved in the morphogenetic assembly pathway from bicones to mature cylindrical forms. Using volumetric compression, gas vesicles were shown to comprise 17% of S39006 cells, whereas in Escherichia coli heterologously expressing the gas vesicle cluster in a deregulated environment, gas vesicles can occupy around half of cellular volume. Gas vesicle production in S39006 and E.coli was exploited to calculate the instantaneous turgor pressure within cultured bacterial cells; the first time this has been performed in either strain. 2016 Journal Article http://hdl.handle.net/20.500.11937/36437 10.1111/1462-2920.13203 Wiley-Blackwell Publishing fulltext |
| spellingShingle | Tashiro, Y. Monson, R. Ramsay, Joshua Salmond, G. Molecular genetic and physical analysis of gas vesicles in buoyant enterobacteria |
| title | Molecular genetic and physical analysis of gas vesicles in buoyant enterobacteria |
| title_full | Molecular genetic and physical analysis of gas vesicles in buoyant enterobacteria |
| title_fullStr | Molecular genetic and physical analysis of gas vesicles in buoyant enterobacteria |
| title_full_unstemmed | Molecular genetic and physical analysis of gas vesicles in buoyant enterobacteria |
| title_short | Molecular genetic and physical analysis of gas vesicles in buoyant enterobacteria |
| title_sort | molecular genetic and physical analysis of gas vesicles in buoyant enterobacteria |
| url | http://hdl.handle.net/20.500.11937/36437 |