Microneedle Enhanced Delivery of Cosmeceutically Relevant Peptides in Human Skin
Peptides and proteins play an important role in skin health and well-being. They are also found to contribute to skin aging and melanogenesis. Microneedles have been shown to substantially enhance skin penetration and may offer an effective means of peptide delivery enhancement. The aim of this inve...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
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Public Library of Science
2014
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/36212 |
| _version_ | 1848754705868521472 |
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| author | Mohammed, Yousuf Yamada, M. Lin, L. Grice, J. Roberts, M. Raphael, A. Benson, Heather Prow, T. |
| author_facet | Mohammed, Yousuf Yamada, M. Lin, L. Grice, J. Roberts, M. Raphael, A. Benson, Heather Prow, T. |
| author_sort | Mohammed, Yousuf |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Peptides and proteins play an important role in skin health and well-being. They are also found to contribute to skin aging and melanogenesis. Microneedles have been shown to substantially enhance skin penetration and may offer an effective means of peptide delivery enhancement. The aim of this investigation was to assess the influence of microneedles on the skin penetration of peptides using fluorescence imaging to determine skin distribution. In particular the effect of peptide chain length (3, 4, 5 amino acid chain length) on passive and MN facilitated skin penetration was investigated. Confocal laser scanning microscopy was used to image fluorescence intensity and the area of penetration of fluorescently tagged peptides. Penetration studies were conducted on excised full thickness human skin in Franz type diffusion cells for 1 and 24 hours. A 2 to 22 fold signal improvement in microneedle enhanced delivery of melanostatin, rigin and pal-KTTKS was observed. To our knowledge this is the first description of microneedle enhanced skin permeation studies on these peptides. |
| first_indexed | 2025-11-14T08:44:40Z |
| format | Journal Article |
| id | curtin-20.500.11937-36212 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:44:40Z |
| publishDate | 2014 |
| publisher | Public Library of Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-362122017-09-13T15:17:38Z Microneedle Enhanced Delivery of Cosmeceutically Relevant Peptides in Human Skin Mohammed, Yousuf Yamada, M. Lin, L. Grice, J. Roberts, M. Raphael, A. Benson, Heather Prow, T. peptide synthesis iontophoresis epidermis dermis signal peptides fluorescence imgaing Confocal laser microscope image analysis Peptides and proteins play an important role in skin health and well-being. They are also found to contribute to skin aging and melanogenesis. Microneedles have been shown to substantially enhance skin penetration and may offer an effective means of peptide delivery enhancement. The aim of this investigation was to assess the influence of microneedles on the skin penetration of peptides using fluorescence imaging to determine skin distribution. In particular the effect of peptide chain length (3, 4, 5 amino acid chain length) on passive and MN facilitated skin penetration was investigated. Confocal laser scanning microscopy was used to image fluorescence intensity and the area of penetration of fluorescently tagged peptides. Penetration studies were conducted on excised full thickness human skin in Franz type diffusion cells for 1 and 24 hours. A 2 to 22 fold signal improvement in microneedle enhanced delivery of melanostatin, rigin and pal-KTTKS was observed. To our knowledge this is the first description of microneedle enhanced skin permeation studies on these peptides. 2014 Journal Article http://hdl.handle.net/20.500.11937/36212 10.1371/journal.pone.0101956 Public Library of Science fulltext |
| spellingShingle | peptide synthesis iontophoresis epidermis dermis signal peptides fluorescence imgaing Confocal laser microscope image analysis Mohammed, Yousuf Yamada, M. Lin, L. Grice, J. Roberts, M. Raphael, A. Benson, Heather Prow, T. Microneedle Enhanced Delivery of Cosmeceutically Relevant Peptides in Human Skin |
| title | Microneedle Enhanced Delivery of Cosmeceutically Relevant Peptides in Human Skin |
| title_full | Microneedle Enhanced Delivery of Cosmeceutically Relevant Peptides in Human Skin |
| title_fullStr | Microneedle Enhanced Delivery of Cosmeceutically Relevant Peptides in Human Skin |
| title_full_unstemmed | Microneedle Enhanced Delivery of Cosmeceutically Relevant Peptides in Human Skin |
| title_short | Microneedle Enhanced Delivery of Cosmeceutically Relevant Peptides in Human Skin |
| title_sort | microneedle enhanced delivery of cosmeceutically relevant peptides in human skin |
| topic | peptide synthesis iontophoresis epidermis dermis signal peptides fluorescence imgaing Confocal laser microscope image analysis |
| url | http://hdl.handle.net/20.500.11937/36212 |