Genetic Regulation of Symbiosis Island Transfer in Mesorhizobium loti
The Mesorhizobium loti symbiosis island (ICEMlSymR7A) is a 500-kb mobile integrative and conjugative element (ICE) that converts non-symbiotic mesorhizobia into strains capable of forming a symbiosis with Lotus corniculatus. ICEMlSymR7A chromosomal excision and conjugative transfer is stimulated by...
| Main Authors: | , |
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| Format: | Book Chapter |
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2015
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| Online Access: | http://hdl.handle.net/20.500.11937/35990 |
| _version_ | 1848754645615247360 |
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| author | Ramsay, Joshua Ronson, C. |
| author_facet | Ramsay, Joshua Ronson, C. |
| author_sort | Ramsay, Joshua |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The Mesorhizobium loti symbiosis island (ICEMlSymR7A) is a 500-kb mobile integrative and conjugative element (ICE) that converts non-symbiotic mesorhizobia into strains capable of forming a symbiosis with Lotus corniculatus. ICEMlSymR7A chromosomal excision and conjugative transfer is stimulated by the quorum-sensing regulator TraR. Quorum sensing and ICEMlSymR7A excision/transfer is inhibited in the majority of the M. loti population by QseM, a recently identified protein antiactivator of TraR. QseM expression is in turn regulated by the DNA-binding protein QseC, which binds and represses the qseM promoter in a concentration-dependent manner. We propose that this complex regulation forms a molecular switch allowing activation of quorum sensing, excision and transfer in a small proportion of cells in the population, rather than the population-wide activation typical of many quorum-sensing systems. |
| first_indexed | 2025-11-14T08:43:42Z |
| format | Book Chapter |
| id | curtin-20.500.11937-35990 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:43:42Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-359902017-09-13T15:35:34Z Genetic Regulation of Symbiosis Island Transfer in Mesorhizobium loti Ramsay, Joshua Ronson, C. The Mesorhizobium loti symbiosis island (ICEMlSymR7A) is a 500-kb mobile integrative and conjugative element (ICE) that converts non-symbiotic mesorhizobia into strains capable of forming a symbiosis with Lotus corniculatus. ICEMlSymR7A chromosomal excision and conjugative transfer is stimulated by the quorum-sensing regulator TraR. Quorum sensing and ICEMlSymR7A excision/transfer is inhibited in the majority of the M. loti population by QseM, a recently identified protein antiactivator of TraR. QseM expression is in turn regulated by the DNA-binding protein QseC, which binds and represses the qseM promoter in a concentration-dependent manner. We propose that this complex regulation forms a molecular switch allowing activation of quorum sensing, excision and transfer in a small proportion of cells in the population, rather than the population-wide activation typical of many quorum-sensing systems. 2015 Book Chapter http://hdl.handle.net/20.500.11937/35990 10.1002/9781119053095.ch21 restricted |
| spellingShingle | Ramsay, Joshua Ronson, C. Genetic Regulation of Symbiosis Island Transfer in Mesorhizobium loti |
| title | Genetic Regulation of Symbiosis Island Transfer in Mesorhizobium loti |
| title_full | Genetic Regulation of Symbiosis Island Transfer in Mesorhizobium loti |
| title_fullStr | Genetic Regulation of Symbiosis Island Transfer in Mesorhizobium loti |
| title_full_unstemmed | Genetic Regulation of Symbiosis Island Transfer in Mesorhizobium loti |
| title_short | Genetic Regulation of Symbiosis Island Transfer in Mesorhizobium loti |
| title_sort | genetic regulation of symbiosis island transfer in mesorhizobium loti |
| url | http://hdl.handle.net/20.500.11937/35990 |