Cell-autonomous complementation of mlo resistance using a biolistic transient expression system
The barley geneMloencodes a prototype of a novel class of plant proteins. Inmlomutants, absence of the 60 kDa wild-type Mlo protein results in broad-spectrum resistance to the powdery mildew fungus,Erysiphe graminisf. sp.hordei. To directly assess its function,Mlowas transiently expressed with a mar...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Published: |
1999
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| Online Access: | http://hdl.handle.net/20.500.11937/35831 |
| _version_ | 1848754602972807168 |
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| author | Shirasu, K. Nielsen, K. Piffanelli, P. Oliver, Richard Schulze-Lefert, P. |
| author_facet | Shirasu, K. Nielsen, K. Piffanelli, P. Oliver, Richard Schulze-Lefert, P. |
| author_sort | Shirasu, K. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The barley geneMloencodes a prototype of a novel class of plant proteins. Inmlomutants, absence of the 60 kDa wild-type Mlo protein results in broad-spectrum resistance to the powdery mildew fungus,Erysiphe graminisf. sp.hordei. To directly assess its function,Mlowas transiently expressed with a marker gene encoding a modified green fluorescent protein (GFP) in leaf epidermal cells ofmloresistant barley lines. Fungal inoculation of epidermal cells transfected with wild-typeMloled to haustorium formation and abundant sporulation. Therefore, expression of the wild-typeMlogene, inmloresistant genotypes, is both necessary and sufficient to restore susceptibility to fungal attack. Complementation ofmloresistance alleles was restricted to single host cells, indicating a cell-autonomous function for the wild-type Mlo protein. We discuss our findings with respect to source–sink relationships of plants and biotrophic fungi and the potentially wide-ranging use of the transient complementation assay to analyse host compatibility and defence in response to powdery mildew attack. |
| first_indexed | 2025-11-14T08:43:02Z |
| format | Journal Article |
| id | curtin-20.500.11937-35831 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T08:43:02Z |
| publishDate | 1999 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-358312017-09-13T16:08:23Z Cell-autonomous complementation of mlo resistance using a biolistic transient expression system Shirasu, K. Nielsen, K. Piffanelli, P. Oliver, Richard Schulze-Lefert, P. The barley geneMloencodes a prototype of a novel class of plant proteins. Inmlomutants, absence of the 60 kDa wild-type Mlo protein results in broad-spectrum resistance to the powdery mildew fungus,Erysiphe graminisf. sp.hordei. To directly assess its function,Mlowas transiently expressed with a marker gene encoding a modified green fluorescent protein (GFP) in leaf epidermal cells ofmloresistant barley lines. Fungal inoculation of epidermal cells transfected with wild-typeMloled to haustorium formation and abundant sporulation. Therefore, expression of the wild-typeMlogene, inmloresistant genotypes, is both necessary and sufficient to restore susceptibility to fungal attack. Complementation ofmloresistance alleles was restricted to single host cells, indicating a cell-autonomous function for the wild-type Mlo protein. We discuss our findings with respect to source–sink relationships of plants and biotrophic fungi and the potentially wide-ranging use of the transient complementation assay to analyse host compatibility and defence in response to powdery mildew attack. 1999 Journal Article http://hdl.handle.net/20.500.11937/35831 10.1046/j.1365-313X.1999.00376.x restricted |
| spellingShingle | Shirasu, K. Nielsen, K. Piffanelli, P. Oliver, Richard Schulze-Lefert, P. Cell-autonomous complementation of mlo resistance using a biolistic transient expression system |
| title | Cell-autonomous complementation of mlo resistance using a biolistic transient expression system |
| title_full | Cell-autonomous complementation of mlo resistance using a biolistic transient expression system |
| title_fullStr | Cell-autonomous complementation of mlo resistance using a biolistic transient expression system |
| title_full_unstemmed | Cell-autonomous complementation of mlo resistance using a biolistic transient expression system |
| title_short | Cell-autonomous complementation of mlo resistance using a biolistic transient expression system |
| title_sort | cell-autonomous complementation of mlo resistance using a biolistic transient expression system |
| url | http://hdl.handle.net/20.500.11937/35831 |